AB233533

Anti-RCBTB1 antibody

Name: Anti-RCBTB1 antibody (ab233533) | Abcam
Brand: Abcam Limited
SKU: AB233533
Price: 605 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RCBTB1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RCBTB1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CLLD7, E4.5, RCBTB1, RCC1 and BTB domain-containing protein 1, Chronic lymphocytic leukemia deletion region gene 7 protein, Regulator of chromosome condensation and BTB domain-containing protein 1, CLL deletion region gene 7 protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCBTB1 antibody (AB233533)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCBTB1 antibody (AB233533)

Formalin-fixed, paraffin-embedded human brain cortex tissue stained for RCBTB1 using ab233533 at 1/100 dilution in immunohistochemical analysis. AEC staining.

Supplier Data

Western blot - Anti-RCBTB1 antibody (AB233533)

All lanes:

Western blot - Anti-RCBTB1 antibody (ab233533) at 1/500 dilution

Lane 1:

KNRK whole cell lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 5:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lane 6:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RCBTB1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8NDN9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RCBTB1 also known as RCC1 and BTB domain-containing protein 1 is a protein with a mass of approximately 74 kDa. Mechanically RCBTB1 functions as a suppressor in ubiquitin-proteasome systems part of the BTB-Kelch protein family implicated in protein degradation processes. Its expression occurs broadly with elevated levels noted in kidney liver and brain tissues indicating a role in various physiological contexts.

Biological function summary

RCBTB1 acts as a regulator in cell cycle and apoptosis pathways. It is an integral part of a complex that modulates transcriptional repression and ubiquitination. Researchers have highlighted its involvement in controlling cellular proliferation making it significant in maintaining cellular homeostasis. RCBTB1 also interacts with other proteins to influence gene expression patterns necessary for cell differentiation.

Pathways

RCBTB1 is notably engaged in the p53 signaling and WNT pathways. These pathways play essential roles in tumor suppression and cell fate decisions respectively. It associates with proteins such as MDM2 in the p53 pathway impacting cellular responses to DNA damage. Through the WNT signaling cascade RCBTB1 influences beta-catenin aiding in the transition between cellular proliferation and differentiation states.

Researchers have linked RCBTB1 to conditions such as cancer and retinitis pigmentosa. Alterations in its function can disrupt tumor suppression mechanisms given its connection to proteins like p53 and MDM2. Furthermore mutations in RCBTB1 have been associated with retinitis pigmentosa where its interaction with other proteins involved in photoreceptor cell maintenance becomes disrupted leading to progressive vision loss.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in cell cycle regulation by chromatin remodeling.

See full target information RCBTB1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406026 PubMed39494721

2024

Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Ouyang,Yaling Li,Haoming Wang,Xiangyang Liu,Xiaoli Tan,Genyuan Xie,Junfa Zeng,Gaofeng Zeng,Qiong Luo,Hong Zhou,Siming Chen,Kai Hou,Jinren Fang,Xia Zhang,Linlin Zhou,Yukun Li,Anbo Gao

Asian Pacific journal of cancer prevention : APJCP 25:1257-1264 PubMed38679985

2024

Expression of CLLD7 and CHC1L Proteins in Oral Epithelial Dysplasia in a Group of Thai Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Patcharanun Wiphakphongpakorn,Rachai Juengsomjit,Sopee Poomsawat,Ounruean Meesakul,Bishwa Prakash Bhattarai,Boworn Klongnoi,Waranun Buajeeb,Siribang-On Piboonniyom Khovidhunkit

European journal of dentistry 18:297-303 PubMed37311552

2023

Preliminary Study on the Expression of CLLD7 and CHC1L Proteins in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Boworn Klongnoi,Bishwa Prakash Bhattarai,Rachai Juengsomjit,Ounruean Meesakul,Sopee Poomsawat,Kajohnkiart Janebodin,Siribang-On Piboonniyom Khovidhunkit

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RCBTB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCBTB1 antibody (AB233533)

Western blot - Anti-RCBTB1 antibody (AB233533)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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