AB152156

Anti-RCC1 antibody

Name: Anti-RCC1 antibody (ab152156) | Abcam
Brand: Abcam Limited
SKU: AB152156

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(1 Publication)

Rabbit Polyclonal RCC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RCC1 aa 1 to C-terminus.

View Alternative Names

CHC1, RCC1, Regulator of chromosome condensation, Cell cycle regulatory protein, Chromosome condensation protein 1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-RCC1 antibody (AB152156)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RCC1 antibody (AB152156)

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, staining RCC1 (green) using ab152156 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody (AB152156)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody (AB152156)

Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue, staining RCC1 using ab152156 at a 1/500 dilution.

Supplier Data

Western blot - Anti-RCC1 antibody (AB152156)

10% SDS PAGE

All lanes:

Western blot - Anti-RCC1 antibody (ab152156) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Lane 4:

HeLa S3 whole cell lysate at 30 µg

Lane 5:

HepG2 whole cell lysate at 30 µg

Lane 6:

MOLT4 whole cell lysate at 30 µg

Lane 7:

Raji whole cell lysate at 30 µg

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-RCC1 antibody (AB152156)

12% SDS PAGE

All lanes:

Western blot - Anti-RCC1 antibody (ab152156) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate/extract at 30 µg

Lane 2:

GL261 whole cell lysate/extract at 30 µg

Predicted band size: 45 kDa,83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RCC1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18754

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Regulator of Chromosome Condensation 1 (RCC1) also known as Ran guanine nucleotide exchange factor is a highly conserved protein. With a molecular mass of approximately 45 kDa RCC1 localizes mainly in the nucleus. This protein functions as a guanine nucleotide exchange factor (GEF) for the small GTPase Ran facilitating the conversion of RanGDP to RanGTP. RCC1 is abundant in cells showing expression in various tissue types reflecting its mechanical role in nuclear transport.

Biological function summary

The protein RCC1 acts as an important player in the regulation of nucleocytoplasmic transport mitosis and other cellular processes. It forms a complex with chromatin and its substrate Ran particularly binding to DNA in a sequence-specific manner. RCC1's activity modifies the Ran gradient across the nuclear envelope which is essential for directing the import and export of proteins and RNA molecules. This gradient is also vital during mitosis for proper chromosomal segregation.

Pathways

RCC1 integrates into the Ran pathway a mechanism significant for cell cycle progression and nucleocytoplasmic transport. Within this pathway RCC1 influences the Ran cycle in partnership with proteins such as RanBP1 and RanGAP. It also plays a part in mitotic spindle assembly through its interactions with spindle assembly factors interlinking with microtubule dynamics. RCC1's function in these pathways highlights its critical involvement in maintaining cellular division and transport activities.

Defects in RCC1 expression or function have connections to tumorigenesis and certain neurological disorders. Aberrations in the Ran signaling pathway mediated by RCC1 can disturb genomic stability linking it to cancer progression. Additionally improper regulation of RCC1 might implicate its association with neurodegenerative conditions where proteins like RanGAP1 and importin beta are also involved. Understanding these relationships may lead to potential therapeutic targets for treating such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed : 11336674, PubMed : 17435751, PubMed : 1944575, PubMed : 20668449, PubMed : 22215983, PubMed : 29042532). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed : 12194828, PubMed : 17435751, PubMed : 22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed : 22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed : 3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed : 17435751, PubMed : 18762580).

See full target information RCC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 13:1064-1068 PubMed28450943

2017

Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanyi Wang,Yuxiang Huang,Renhua Guo,Yongbiao Liu,Yayun Qian,Dan Liu,Xiaojun Dai,Zheng Wei,Feng Jin,Yanqing Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RCC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RCC1 antibody (AB152156)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody (AB152156)

Western blot - Anti-RCC1 antibody (AB152156)

Western blot - Anti-RCC1 antibody (AB152156)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise