AB70787

Anti-RCC2 antibody

Name: Anti-RCC2 antibody (ab70787) | Abcam
Brand: Abcam Limited
SKU: AB70787
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RCC2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RCC2.

View Alternative Names

KIAA1470, TD60, RCC2, Protein RCC2, RCC1-like protein TD-60, Telophase disk protein of 60 kDa

2 Images

Unknown

Immunoprecipitation - Anti-RCC2 antibody (AB70787)

HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab70787 at 1 µg/ml for WB, and for IP at 3 µg/mg lysate.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RCC2 antibody (ab70787)

Predicted band size: 56 kDa

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Unknown

Western blot - Anti-RCC2 antibody (AB70787)

All lanes:

Western blot - Anti-RCC2 antibody (ab70787) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 56 kDa

Observed band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RCC2. The exact immunogen used to generate this antibody is proprietary information.

Q9P258

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RCC2 also known as TD-60 or Regulator of Chromosome Condensation 2 is a protein involved in cell cycle regulation. It has a mass of approximately 104 kDa. RCC2 is expressed in various tissues with higher levels often observed in proliferating cells. Mechanically RCC2 acts as a regulator of mitosis and contributes to centrosome cohesion and proper chromosomal alignment during cell division by acting as a guanine nucleotide exchange factor (GEF).

Biological function summary

RCC2 plays a role in the coordination of mitotic events. It is part of the chromosomal passenger complex (CPC) which includes other proteins such as Aurora B kinase INCENP and Survivin. These proteins collectively regulate the alignment and segregation of chromosomes during cell division ensuring accurate distribution of genetic material to daughter cells. RCC2's function in this complex helps stabilize the spindle apparatus and ensures timely cytokinesis.

Pathways

RCC2 is significantly involved in cell cycle and mitosis pathways. It interacts closely with proteins such as Aurora B kinase in the regulation of mitotic processes. RCC2 controls the activation of small GTPases which are critical for cellular dynamics during division. Through these interactions RCC2 affects centrosome function and maintenance integrating itself into the broader network of cell cycle regulation.

RCC2 is associated with cancer where its dysregulation may contribute to abnormal cell proliferation. Overexpression or modifications in RCC2 can result in mitotic errors leading to aneuploidy a feature often observed in tumorigenic cells. In particular changes in RCC2 expression levels have been linked to colorectal cancer. It interacts with other proteins like Aurora B kinase in this context exacerbating chromosomal instability and enhancing cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional protein that may affect its functions by regulating the activity of small GTPases, such as RAC1 and RALA (PubMed : 12919680, PubMed : 25074804, PubMed : 26158537, PubMed : 28869598). Required for normal progress through the cell cycle, both during interphase and during mitosis (PubMed : 12919680, PubMed : 23388455, PubMed : 26158537). Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles (PubMed : 12919680, PubMed : 26158537). Required for normal organization of the microtubule cytoskeleton in interphase cells (PubMed : 23388455). Functions as guanine nucleotide exchange factor (GEF) for RALA (PubMed : 26158537). Interferes with the activation of RAC1 by guanine nucleotide exchange factors (PubMed : 25074804). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed : 25074804, PubMed : 28869598). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (PubMed : 25074804, PubMed : 28869598).

See full target information RCC2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Physiological genomics 53:385-394 PubMed34297635

2021

Lysine demethylase KDM6B regulates HIF-1α-mediated systemic and cellular responses to intermittent hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jayasri Nanduri,Ning Wang,Benjamin L Wang,Nanduri R Prabhakar

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:13573-13585 PubMed32803782

2020

MicroRNA-191 modulates cisplatin-induced DNA damage response by targeting RCC2.

Applications

Unspecified application

Species

Unspecified reactive species

Xianrong Xu,Xiaofeng Zhou,Jianyun Zhang,Hongjuan Li,Yifei Cao,Xiaohua Tan,Xinqiang Zhu,Jun Yang

View all publications

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Product promise

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Anti-RCC2 antibody

Immunoprecipitation - Anti-RCC2 antibody (AB70787)

Western blot - Anti-RCC2 antibody (AB70787)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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