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AB70788

Anti-RCC2 antibody

5

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal RCC2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RCC2 aa 450 to C-terminus.

View Alternative Names

KIAA1470, TD60, RCC2, Protein RCC2, RCC1-like protein TD-60, Telophase disk protein of 60 kDa

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC2 antibody (AB70788)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC2 antibody (AB70788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection : DAB.

Western blot - Anti-RCC2 antibody (AB70788)
  • WB

Unknown

Western blot - Anti-RCC2 antibody (AB70788)

All lanes:

Western blot - Anti-RCC2 antibody (ab70788) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RCC2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P258

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RCC2 also known as TD-60 or Regulator of Chromosome Condensation 2 is a protein involved in cell cycle regulation. It has a mass of approximately 104 kDa. RCC2 is expressed in various tissues with higher levels often observed in proliferating cells. Mechanically RCC2 acts as a regulator of mitosis and contributes to centrosome cohesion and proper chromosomal alignment during cell division by acting as a guanine nucleotide exchange factor (GEF).
Biological function summary

RCC2 plays a role in the coordination of mitotic events. It is part of the chromosomal passenger complex (CPC) which includes other proteins such as Aurora B kinase INCENP and Survivin. These proteins collectively regulate the alignment and segregation of chromosomes during cell division ensuring accurate distribution of genetic material to daughter cells. RCC2's function in this complex helps stabilize the spindle apparatus and ensures timely cytokinesis.

Pathways

RCC2 is significantly involved in cell cycle and mitosis pathways. It interacts closely with proteins such as Aurora B kinase in the regulation of mitotic processes. RCC2 controls the activation of small GTPases which are critical for cellular dynamics during division. Through these interactions RCC2 affects centrosome function and maintenance integrating itself into the broader network of cell cycle regulation.

RCC2 is associated with cancer where its dysregulation may contribute to abnormal cell proliferation. Overexpression or modifications in RCC2 can result in mitotic errors leading to aneuploidy a feature often observed in tumorigenic cells. In particular changes in RCC2 expression levels have been linked to colorectal cancer. It interacts with other proteins like Aurora B kinase in this context exacerbating chromosomal instability and enhancing cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that may affect its functions by regulating the activity of small GTPases, such as RAC1 and RALA (PubMed : 12919680, PubMed : 25074804, PubMed : 26158537, PubMed : 28869598). Required for normal progress through the cell cycle, both during interphase and during mitosis (PubMed : 12919680, PubMed : 23388455, PubMed : 26158537). Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles (PubMed : 12919680, PubMed : 26158537). Required for normal organization of the microtubule cytoskeleton in interphase cells (PubMed : 23388455). Functions as guanine nucleotide exchange factor (GEF) for RALA (PubMed : 26158537). Interferes with the activation of RAC1 by guanine nucleotide exchange factors (PubMed : 25074804). Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions (PubMed : 25074804, PubMed : 28869598). Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro) (PubMed : 25074804, PubMed : 28869598).
See full target information RCC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 24:725 PubMed36340602

2022

A pan‑cancer analysis of RCC2 and its interaction with HMGA2 protein in an in vitro model of colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guiyuan Gu,Yuhong Wang,Yucheng Shen,Yan Ma,Hongli Yang,Shan Huang,Lin Hu

EMBO reports 19: PubMed29794156

2018

The β3-integrin endothelial adhesome regulates microtubule-dependent cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel J Atkinson,Aleksander M Gontarczyk,Abdullah Aa Alghamdi,Tim S Ellison,Robert T Johnson,Wesley J Fowler,Benjamin M Kirkup,Bernardo C Silva,Bronwen E Harry,Jochen G Schneider,Katherine N Weilbaecher,Mette M Mogensen,Mark D Bass,Maddy Parsons,Dylan R Edwards,Stephen D Robinson
View all publications
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