Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)

Rabbit Recombinant Monoclonal RCN1/RCN antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (AB251515), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Tested	Tested
Rat	Expected	Expected	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information RCN1

Function

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

Alternative names

RCN, RCN1, Reticulocalbin-1

Recommended products

Rabbit Recombinant Monoclonal RCN1/RCN antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR17163-117
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab251515 is the carrier-free version of Anti-RCN1/RCN antibody [EPR17163-117] ab210404.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RCN1 also known as reticulocalbin-1 or RCN is a calcium-binding protein located in the lumen of the endoplasmic reticulum (ER). It belongs to the CREC family of proteins which are characterized by their EF-hand motifs that mediate calcium binding. RCN1 has a molecular mass of around 37 kDa. This protein is expressed in various tissues including the pancreas heart and brain suggesting its involvement in diverse cellular functions. Within the ER RCN1 interacts with other proteins aiding in their proper folding and assembly.

Biological function summary

RCN1 plays a role in cellular calcium homeostasis and may influence processes such as protein folding and trafficking. It is not known to be a part of a larger complex but it interacts with other ER-resident proteins. Maintaining calcium levels is important within the ER where RCN1 helps buffer calcium concentrations. This function supports processes that are sensitive to calcium fluctuations such as signal transduction and stress responses.

Pathways

The role of RCN1 in cellular mechanisms connects it to important pathways like the calcium signaling pathway and the unfolded protein response pathway. In the calcium signaling pathway RCN1 aids in regulating calcium levels within the ER affecting downstream signaling events. Meanwhile in the unfolded protein response pathway RCN1 participates by ensuring the proper folding of proteins particularly during times of stress when misfolded proteins accumulate. RCN1's interaction with calumenin another calcium-binding protein highlights its involvement in these pathways.

Associated diseases and disorders

RCN1 has associations with cardiovascular disease and some forms of cancer. In cardiovascular disease altered expression of RCN1 may impact calcium signaling and lead to dysfunction in heart cells. Similarly in certain cancers changes in RCN1 expression levels could affect cellular proliferation via calcium-regulated pathways. The interaction between RCN1 and calreticulin a multifunctional protein involved in calcium homeostasis and immune response further connects RCN1's influence on disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Western blot - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using the same antibody clone in a different buffer formulation (Anti-RCN1/RCN antibody [EPR17163-117] ab210404).
Lanes 1- 4: Merged signal (red and green). Green - Anti-RCN1/RCN antibody [EPR17163-117] ab210404 observed at 45 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.
Anti-RCN1/RCN antibody [EPR17163-117] ab210404 was shown to react with RCN1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human RCN1 (RCN) knockout HEK-293T cell line ab266098 (knockout cell lysate Human RCN1 (RCN) knockout HEK-293T cell lysate ab258172) was used. Wild-type HEK-293T and RCN1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-RCN1/RCN antibody [EPR17163-117] ab210404 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-RCN1/RCN antibody [EPR17163-117] (Anti-RCN1/RCN antibody [EPR17163-117] ab210404) at 1/2000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: RCN1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human RCN1 (RCN) knockout HEK-293T cell line (Human RCN1 (RCN) knockout HEK-293T cell line ab266098)
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on neurons of Human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Western blot - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8416973).
All lanes: Western blot - Anti-RCN1/RCN antibody [EPR17163-117] (Anti-RCN1/RCN antibody [EPR17163-117] ab210404) at 1/5000 dilution
Lane 1: Human placenta lysate at 20 µg
Lane 2: Human fetal kidney lysate at 20 µg
Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 5: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 6: E14 mouse embryo lysate at 20 µg
Lane 7: E14 rat embryo lysate at 20 µg
Lane 8: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa, 46 kDa
Exposure time: 30s
Western blot - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-8: 1 minute; Lanes 9-11: 10 seconds.
All lanes: Western blot - Anti-RCN1/RCN antibody [EPR17163-117] (Anti-RCN1/RCN antibody [EPR17163-117] ab210404) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
Lane 5: Rat brain lysate at 10 µg
Lane 6: Rat heart lysate at 10 µg
Lane 7: Rat kidney lysate at 10 µg
Lane 8: Rat spleen lysate at 10 µg
Lane 9: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 10: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 11: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa, 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on tumor cells of Human lung adenocarcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat hippocampus is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.RCN1/RCN was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) whole cell lysate with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/50 dilution. Western blot was performed from the immunoprecipitate using Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution. Lane 1: 293T whole cell lysate, 10µg (Input). Lane 2: Anti-RCN1/RCN antibody [EPR17163-117] ab210404 IP in 293T whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RCN1/RCN antibody [EPR17163-117] ab210404 in 293T whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163-117] (Anti-RCN1/RCN antibody [EPR17163-117] ab210404)
Predicted band size: 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/700 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilution was used as the secondary antibody.
Cells were permeabilised with 90% methanol (diluted with 1X PBS).
Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163-117] - BSA and Azide free (ab251515)
This data was developed using Anti-RCN1/RCN antibody [EPR17163-117] ab210404, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling RCN1/RCN with Anti-RCN1/RCN antibody [EPR17163-117] ab210404 at 1/700 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilution was used as the secondary antibody.
Cells were permeabilised with 90% methanol (diluted with 1X PBS).