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Rabbit Recombinant Monoclonal RCN1/RCN antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996), expandable thumbnail
  • Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996), expandable thumbnail
  • Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/250
Notes

-

Species
Rat
Dilution info
1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/70
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/70
Notes

-

Species
Rat
Dilution info
1/70
Notes

-

Tested
Tested

Species
Human
Dilution info
1/800
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/800
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/800
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

Alternative names

RCN, RCN1, Reticulocalbin-1

Recommended products

Rabbit Recombinant Monoclonal RCN1/RCN antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17163
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RCN1 also known as reticulocalbin-1 or RCN is a calcium-binding protein located in the lumen of the endoplasmic reticulum (ER). It belongs to the CREC family of proteins which are characterized by their EF-hand motifs that mediate calcium binding. RCN1 has a molecular mass of around 37 kDa. This protein is expressed in various tissues including the pancreas heart and brain suggesting its involvement in diverse cellular functions. Within the ER RCN1 interacts with other proteins aiding in their proper folding and assembly.

Biological function summary

RCN1 plays a role in cellular calcium homeostasis and may influence processes such as protein folding and trafficking. It is not known to be a part of a larger complex but it interacts with other ER-resident proteins. Maintaining calcium levels is important within the ER where RCN1 helps buffer calcium concentrations. This function supports processes that are sensitive to calcium fluctuations such as signal transduction and stress responses.

Pathways

The role of RCN1 in cellular mechanisms connects it to important pathways like the calcium signaling pathway and the unfolded protein response pathway. In the calcium signaling pathway RCN1 aids in regulating calcium levels within the ER affecting downstream signaling events. Meanwhile in the unfolded protein response pathway RCN1 participates by ensuring the proper folding of proteins particularly during times of stress when misfolded proteins accumulate. RCN1's interaction with calumenin another calcium-binding protein highlights its involvement in these pathways.

Associated diseases and disorders

RCN1 has associations with cardiovascular disease and some forms of cancer. In cardiovascular disease altered expression of RCN1 may impact calcium signaling and lead to dysfunction in heart cells. Similarly in certain cancers changes in RCN1 expression levels could affect cellular proliferation via calcium-regulated pathways. The interaction between RCN1 and calreticulin a multifunctional protein involved in calcium homeostasis and immune response further connects RCN1's influence on disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996), expandable thumbnail

    Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Blocking and diluting buffer was 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

    All lanes: Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/10000 dilution

    Lane 1: 293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 44 kDa, 46 kDa

    Exposure time: 1min

  • Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996), expandable thumbnail

    Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    RCN1/RCN was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.

    Lane 2: HeLa whole cell extract.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab198996 in HeLa whole cell extract.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Predicted band size: 39 kDa

    Observed band size: 44 kDa, 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996), expandable thumbnail

    Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Blocking and diluting buffer was 5% NFDM/TBST.
    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

    All lanes: Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/1000 dilution

    Lane 1: Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

    Lane 2: Rat heart tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 44 kDa, 46 kDa

    Exposure time: 1min

  • Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

    Intracellular Flow Cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

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Product protocols

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