AB198996

Anti-RCN1/RCN antibody [EPR17163] - C-terminal

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal RCN1/RCN antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RCN, RCN1, Reticulocalbin-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Intracellular Flow Cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluorr^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Supplier Data

Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

RCN1/RCN was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.

Lane 2 : HeLa whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198996 in HeLa whole cell extract.

The expression profile observed is consistent with what has been described in the literature PMID : 8416973.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Predicted band size: 39 kDa

Observed band size: 44 kDa,46 kDa

false

Supplier Data

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Blocking and diluting buffer was 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature PMID : 8416973.

All lanes:

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/10000 dilution

Lane 1:

293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 44 kDa,46 kDa

true

Exposure time: 1min

Supplier Data

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Blocking and diluting buffer was 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID : 8416973.

All lanes:

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/1000 dilution

Lane 1:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 44 kDa,46 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17163

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RCN1 also known as reticulocalbin-1 or RCN is a calcium-binding protein located in the lumen of the endoplasmic reticulum (ER). It belongs to the CREC family of proteins which are characterized by their EF-hand motifs that mediate calcium binding. RCN1 has a molecular mass of around 37 kDa. This protein is expressed in various tissues including the pancreas heart and brain suggesting its involvement in diverse cellular functions. Within the ER RCN1 interacts with other proteins aiding in their proper folding and assembly.

Biological function summary

RCN1 plays a role in cellular calcium homeostasis and may influence processes such as protein folding and trafficking. It is not known to be a part of a larger complex but it interacts with other ER-resident proteins. Maintaining calcium levels is important within the ER where RCN1 helps buffer calcium concentrations. This function supports processes that are sensitive to calcium fluctuations such as signal transduction and stress responses.

Pathways

The role of RCN1 in cellular mechanisms connects it to important pathways like the calcium signaling pathway and the unfolded protein response pathway. In the calcium signaling pathway RCN1 aids in regulating calcium levels within the ER affecting downstream signaling events. Meanwhile in the unfolded protein response pathway RCN1 participates by ensuring the proper folding of proteins particularly during times of stress when misfolded proteins accumulate. RCN1's interaction with calumenin another calcium-binding protein highlights its involvement in these pathways.

RCN1 has associations with cardiovascular disease and some forms of cancer. In cardiovascular disease altered expression of RCN1 may impact calcium signaling and lead to dysfunction in heart cells. Similarly in certain cancers changes in RCN1 expression levels could affect cellular proliferation via calcium-regulated pathways. The interaction between RCN1 and calreticulin a multifunctional protein involved in calcium homeostasis and immune response further connects RCN1's influence on disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

See full target information RCN1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell stress & chaperones 30:100084 PubMed40412548

2025

Heat shock induces alternative polyadenylation through dynamic DNA methylation and chromatin looping.

Applications

Unspecified application

Species

Unspecified reactive species

Emily E Fink,Yi Zhang,Briana Santo,Anwita Siddavatam,Rosie Ou,Vishal Nanavaty,Byron H Lee,Angela H Ting

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RCN1/RCN antibody [EPR17163] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Flow Cytometry (Intracellular) - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Western blot - Anti-RCN1/RCN antibody [EPR17163] - C-terminal (AB198996)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Multiplex Kits

Product promise