Rabbit Recombinant Monoclonal RCN1/RCN antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.
Images
![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (AB250748), expandable thumbnail](https://content.abcam.com/products/images/rcn1-rcn-antibody-epr19193-bsa-and-azide-free-ab250748--western-blot-img105794.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (AB250748)")
![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (AB250748), expandable thumbnail](https://content.abcam.com/products/images/rcn1-rcn-antibody-epr19193-bsa-and-azide-free-ab250748--western-blot-img10807.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (AB250748)")
![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (AB250748), expandable thumbnail](https://content.abcam.com/products/images/rcn1-rcn-antibody-epr19193-bsa-and-azide-free-ab250748--western-blot-img34853.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (AB250748)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20). |
Species Mouse | Dilution info - | Notes Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20). |
Species Rat | Dilution info - | Notes Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20). |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Alternative names
RCN, RCN1, Reticulocalbin-1
Recommended products
Rabbit Recombinant Monoclonal RCN1/RCN antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19193
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250748 is the carrier-free version of Anti-RCN1/RCN antibody [EPR19193] ab184441.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RCN1 also known as reticulocalbin-1 or RCN is a calcium-binding protein located in the lumen of the endoplasmic reticulum (ER). It belongs to the CREC family of proteins which are characterized by their EF-hand motifs that mediate calcium binding. RCN1 has a molecular mass of around 37 kDa. This protein is expressed in various tissues including the pancreas heart and brain suggesting its involvement in diverse cellular functions. Within the ER RCN1 interacts with other proteins aiding in their proper folding and assembly.
- Biological function summary
RCN1 plays a role in cellular calcium homeostasis and may influence processes such as protein folding and trafficking. It is not known to be a part of a larger complex but it interacts with other ER-resident proteins. Maintaining calcium levels is important within the ER where RCN1 helps buffer calcium concentrations. This function supports processes that are sensitive to calcium fluctuations such as signal transduction and stress responses.
- Pathways
The role of RCN1 in cellular mechanisms connects it to important pathways like the calcium signaling pathway and the unfolded protein response pathway. In the calcium signaling pathway RCN1 aids in regulating calcium levels within the ER affecting downstream signaling events. Meanwhile in the unfolded protein response pathway RCN1 participates by ensuring the proper folding of proteins particularly during times of stress when misfolded proteins accumulate. RCN1's interaction with calumenin another calcium-binding protein highlights its involvement in these pathways.
- Associated diseases and disorders
RCN1 has associations with cardiovascular disease and some forms of cancer. In cardiovascular disease altered expression of RCN1 may impact calcium signaling and lead to dysfunction in heart cells. Similarly in certain cancers changes in RCN1 expression levels could affect cellular proliferation via calcium-regulated pathways. The interaction between RCN1 and calreticulin a multifunctional protein involved in calcium homeostasis and immune response further connects RCN1's influence on disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748), expandable thumbnail](https://content.abcam.com/products/images/rcn1-rcn-antibody-epr19193-bsa-and-azide-free-ab250748--western-blot-img105794.jpg "Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)")
Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)
This data was developed using Anti-RCN1/RCN antibody [EPR19193] ab184441, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-3: 30 seconds; Lane 4: 3 minutes.
All lanes: Western blot - Anti-RCN1/RCN antibody [EPR19193] (Anti-RCN1/RCN antibody [EPR19193] ab184441) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
Lane 4: Human fetal spleen lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa, 46 kDa
![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748), expandable thumbnail](https://content.abcam.com/products/images/rcn1-rcn-antibody-epr19193-bsa-and-azide-free-ab250748--western-blot-img10807.jpg "Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)")
Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)
This data was developed using Anti-RCN1/RCN antibody [EPR19193] ab184441, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-6: 8 seconds; Lanes 7 and 8: 30 seconds; Lane 9: 3 minutes.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8416973).
All lanes: Western blot - Anti-RCN1/RCN antibody [EPR19193] (Anti-RCN1/RCN antibody [EPR19193] ab184441) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: E14 mouse embryo lysate at 20 µg
Lane 5: E14 rat embryo lysate at 20 µg
Lane 6: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 7: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 8: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 9: Rat cerebral cortex lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa, 46 kDa
![Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748), expandable thumbnail](https://content.abcam.com/products/images/rcn1-rcn-antibody-epr19193-bsa-and-azide-free-ab250748--western-blot-img34853.jpg "Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)")
Western blot - Anti-RCN1/RCN antibody [EPR19193] - BSA and Azide free (ab250748)
This data was developed using Anti-RCN1/RCN antibody [EPR19193] ab184441, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-6: 3 minutes Lanes 7 and 8: 30 seconds; Lanes 9 and 10: 1 second.
All lanes: Western blot - Anti-RCN1/RCN antibody [EPR19193] (Anti-RCN1/RCN antibody [EPR19193] ab184441) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
Lane 5: Rat heart lysate at 10 µg
Lane 6: Rat spleen lysate at 10 µg
Lane 7: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 8: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 9: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 10: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa, 46 kDa
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