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AB171946

Anti-RD3 antibody [EPR12169(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal RD3 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

C1orf36, RD3, Protein RD3, Retinal degeneration protein 3

1 Images
Western blot - Anti-RD3 antibody [EPR12169(B)] (AB171946)
  • WB

Supplier Data

Western blot - Anti-RD3 antibody [EPR12169(B)] (AB171946)

All lanes:

Western blot - Anti-RD3 antibody [EPR12169(B)] (ab171946) at 1/1000 dilution

Lane 1:

Mouse eyeball lysate at 10 µg

Lane 2:

Rat eyeball lysate at 10 µg

Predicted band size: 23 kDa

true

  • Carrier free

    Anti-RD3 antibody [EPR12169(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12169(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RD3 also known as retinol dehydrogenase 3 is a critical protein in visual processes. It possesses a molecular mass of approximately 25 kDa. RD3 is expressed mainly in retinal tissues where it plays an important role. The protein is necessary for proper phototransduction through its role in the retinoid cycle which is important for our visual perception.
Biological function summary

RD3 interacts with other proteins and retinoid intermediates to facilitate the efficient conversion of all-trans-retinol into 11-cis-retinal. This function supports the regeneration of visual pigments critical for light absorption. RD3 does not function as part of a larger complex; instead it works independently in its enzymatic role. Its activity ensures continuous visual cycle operation which is fundamental for maintaining normal vision.

Pathways

RD3 operates within the visual cycle known as the retinoid cycle. This cycle is essential for converting light into visual signals in the retina. RD3 is closely related to proteins like RDH5 in this cycle to maintain the necessary retinoid balance. RDH5 works alongside RD3 reflecting the intricate network of enzymes responsible for recycling visual pigments that detect light.

RD3's function is linked to retinal dystrophies. Mutations or dysfunctions in RD3 can lead to severe vision impairments such as Leber congenial amaurosis a hereditary retinal degenerative disorder. Furthermore RD3's relationship with RDH5 can influence the severity of visual impairment as both proteins are important for the retinoid cycle highlighting the importance of RD3 in maintaining ocular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in the regulation of enzymes involved in nucleotide cycle in photoreceptors (PubMed : 21078983, PubMed : 21928830, PubMed : 27471269, PubMed : 29515371, PubMed : 30559291). Inhibits the basal catalytic activity and the GCAP-stimulated activity of GUCY2D and GUCY2F, two retinal guanylyl cyclases involved in the production of cGMP in photoreceptors (PubMed : 21928830, PubMed : 27471269, PubMed : 29515371, PubMed : 30559291). Involved in the transport of GUCY2D and GUCY2F to their target sites in the photoreceptor outer segment (PubMed : 21078983). Up-regulates the activity of GUK1, a kinase that also plays an essential role for recycling GMP and indirectly, cGMP (PubMed : 29515371). Plays an important role for the survival of rods and cones in the retina (By similarity).
See full target information RD3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mitochondrion 34:84-90 PubMed28216230

2017

Activation of a cryptic splice site in the mitochondrial elongation factor GFM1 causes combined OXPHOS deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Mariella T Simon,Bobby G Ng,Marisa W Friederich,Raymond Y Wang,Monica Boyer,Martin Kircher,Renata Collard,Kati J Buckingham,Richard Chang,Jay Shendure,Deborah A Nickerson,Michael J Bamshad,Johan L K Van Hove,Hudson H Freeze,Jose E Abdenur
View all publications
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Product promise

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