AB249665

Anti-RD3 antibody [EPR12169(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RD3 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

C1orf36, RD3, Protein RD3, Retinal degeneration protein 3

1 Images

Supplier Data

Western blot - Anti-RD3 antibody [EPR12169(B)] - BSA and Azide free (AB249665)

This data was developed using ab171946, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RD3 antibody [EPR12169(B)] (<a href='/en-us/products/primary-antibodies/rd3-antibody-epr12169b-ab171946'>ab171946</a>) at 1/1000 dilution

Lane 1:

Mouse eyeball lysate at 10 µg

Lane 2:

Rat eyeball lysate at 10 µg

Predicted band size: 23 kDa

true

Unconjugated

Anti-RD3 antibody [EPR12169(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12169(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249665 is the carrier-free version of ab171946.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RD3 also known as retinol dehydrogenase 3 is a critical protein in visual processes. It possesses a molecular mass of approximately 25 kDa. RD3 is expressed mainly in retinal tissues where it plays an important role. The protein is necessary for proper phototransduction through its role in the retinoid cycle which is important for our visual perception.

Biological function summary

RD3 interacts with other proteins and retinoid intermediates to facilitate the efficient conversion of all-trans-retinol into 11-cis-retinal. This function supports the regeneration of visual pigments critical for light absorption. RD3 does not function as part of a larger complex; instead it works independently in its enzymatic role. Its activity ensures continuous visual cycle operation which is fundamental for maintaining normal vision.

Pathways

RD3 operates within the visual cycle known as the retinoid cycle. This cycle is essential for converting light into visual signals in the retina. RD3 is closely related to proteins like RDH5 in this cycle to maintain the necessary retinoid balance. RDH5 works alongside RD3 reflecting the intricate network of enzymes responsible for recycling visual pigments that detect light.

RD3's function is linked to retinal dystrophies. Mutations or dysfunctions in RD3 can lead to severe vision impairments such as Leber congenial amaurosis a hereditary retinal degenerative disorder. Furthermore RD3's relationship with RDH5 can influence the severity of visual impairment as both proteins are important for the retinoid cycle highlighting the importance of RD3 in maintaining ocular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a critical role in the regulation of enzymes involved in nucleotide cycle in photoreceptors (PubMed : 21078983, PubMed : 21928830, PubMed : 27471269, PubMed : 29515371, PubMed : 30559291). Inhibits the basal catalytic activity and the GCAP-stimulated activity of GUCY2D and GUCY2F, two retinal guanylyl cyclases involved in the production of cGMP in photoreceptors (PubMed : 21928830, PubMed : 27471269, PubMed : 29515371, PubMed : 30559291). Involved in the transport of GUCY2D and GUCY2F to their target sites in the photoreceptor outer segment (PubMed : 21078983). Up-regulates the activity of GUK1, a kinase that also plays an essential role for recycling GMP and indirectly, cGMP (PubMed : 29515371). Plays an important role for the survival of rods and cones in the retina (By similarity).

See full target information RD3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RD3 antibody [EPR12169(B)] - BSA and Azide free

Western blot - Anti-RD3 antibody [EPR12169(B)] - BSA and Azide free (AB249665)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise