AB101457

Anti-RDH5 antibody

Name: Anti-RDH5 antibody (ab101457) | Abcam
Brand: Abcam Limited
SKU: AB101457

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(2 Publications)

Rabbit Polyclonal RDH5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RDH5 aa 100-250.

View Alternative Names

HSD17B9, RDH1, SDR9C5, RDH5, Retinol dehydrogenase 5, 11-cis retinol dehydrogenase, 9-cis retinol dehydrogenase, Short chain dehydrogenase/reductase family 9C member 5, 11-cis RDH, 11-cis RoDH, 9cRDH

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RDH5 antibody (AB101457)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RDH5 antibody (AB101457)

Immunohistochemical analysis of RDH5 in paraffin-embedded Cal27 xenograft, using ab101457 at 1/100 dilution.

Unknown

Western blot - Anti-RDH5 antibody (AB101457)

10% SDS-PAGE

All lanes:

Western blot - Anti-RDH5 antibody (ab101457) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RDH5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q92781

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use 10mM Citrate buffer or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RDH5 also known as retinol dehydrogenase 5 or 11-cis retinol dehydrogenase is an enzyme that plays an important role in the visual cycle. It has a molecular mass of approximately 35 kDa and primarily functions in the conversion of 11-cis-retinol to 11-cis-retinal which is a significant step in the biosynthesis of visual pigments. RDH5 is expressed mainly in the retinal pigment epithelium of the eye where it contributes to maintaining proper vision.

Biological function summary

11-cis retinol dehydrogenase facilitates the regeneration of 11-cis-retinal from 11-cis-retinol a critical process for photoreceptor cell function and visual cycle continuity. RDH5 operates as part of a complex of enzymes involved in the retinoid cycle which is essential for maintaining a continuous supply of 11-cis-retinal. Without RDH5 the regeneration of 11-cis-retinal would be ineffective reducing the ability to see especially in low-light conditions.

Pathways

The retinol dehydrogenase 5 enzyme plays a major role in the visual cycle pathway. This pathway is necessary for converting retinoids into visual chromophores that allow vision. RDH5 interacts closely with other key enzymes such as RPE65 and RDH12 which also participate in retinoid transformations. The proper functioning of the visual cycle pathway depends on the actions of these enzymes to ensure the availability of 11-cis-retinal for photoreceptor function.

Defects in the RDH5 gene can lead to retinal degenerative diseases like fundus albipunctatus and retinitis pigmentosa. These conditions are characterized by night blindness and progressive vision loss. Mutations in RDH5 affect the synthesis of 11-cis-retinal disrupting the visual cycle and contributing to these diseases. RDH5 shares a pathway with RPE65 another protein implicated in retinal disorders highlighting the interconnectedness of proteins involved in the visual cycle and their impact on eye health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalyzes the oxidation of cis-isomers of retinol, including 11-cis-, 9-cis-, and 13-cis-retinol in an NAD-dependent manner (PubMed : 10588954, PubMed : 11675386, PubMed : 9115228, PubMed : 9931293). Has no activity towards all-trans retinal (By similarity). Plays a significant role in 11-cis retinol oxidation in the retinal pigment epithelium cells (RPE). Also recognizes steroids (androsterone, androstanediol) as its substrates (PubMed : 29541409, PubMed : 9931293).

See full target information RDH5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 61:9 PubMed32049341

2020

Increased Rate of Retinal Pigment Epithelial Cell Migration and Pro-Angiogenic Potential Ensuing From Reduced Cystatin C Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Emil Carlsson,Wasu Supharattanasitthi,Malcolm Jackson,Luminita Paraoan

Molecular vision 23:60-89 PubMed28356702

2017

Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells.

Applications

Unspecified application

Species

Human

William Samuel,Cynthia Jaworski,Olga A Postnikova,R Krishnan Kutty,Todd Duncan,Li Xuan Tan,Eugenia Poliakov,Aparna Lakkaraju,T Michael Redmond

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RDH5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RDH5 antibody (AB101457)

Western blot - Anti-RDH5 antibody (AB101457)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise