Rabbit Polyclonal REBOV GP antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Reston ebolavirus samples. Immunogen corresponding to Synthetic Peptide within Reston ebolavirus - Reston (1989) GP.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Reston ebolavirus - Reston (1989) GP. The exact immunogen used to generate this antibody is proprietary information. Database link Q66800
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment - Reston ebolavirus | Tested |
| Reston ebolavirus | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Reston ebolavirus | Dilution info 0.5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Reston ebolavirus | Dilution info - | Notes - |
Target data
Function
Small/secreted glycoprotein. Seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils. Delta-peptide. Viroporin that permeabilizes mammalian cell plasma membranes. It acts by altering permeation of ionic compounds and small molecules. This activity may lead to viral enterotoxic activity.
Alternative names
Pre-small/secreted glycoprotein, pre-sGP, GP
Recommended products
Rabbit Polyclonal REBOV GP antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Reston ebolavirus samples. Immunogen corresponding to Synthetic Peptide within Reston ebolavirus - Reston (1989) GP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Reston ebolavirus - Reston (1989) GP. The exact immunogen used to generate this antibody is proprietary information. Database link Q66800
- Carrier free
- Yes
- Purification technique
- Affinity purification Immunogen
- Specificity
ab190658 detects recombinant REBOV GP without the transmembrane region (REBOV rGPdTM). No cross reactivity to Sudan ebolavirus (SEBOV) GP, Zaire ebolavirus (ZEBOV) GP, Bundibugyo (BEBOV) GP or Marburg (MARV) GP, was observed in Western blot analysis.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
REBOV GP also known as Reston virus glycoprotein is a critical structural protein of the Reston virus. This glycoprotein approximately 150 kDa in mass plays a role in viral attachment and entry into host cells. It is expressed on the surface of the virus envelope facilitating interaction with host cell receptors. The membrane-bound form is often essential for the virus's ability to invade host cells influencing virulence and pathogenicity.
- Biological function summary
The Reston virus glycoprotein mediates the fusion process of the viral envelope with the host cell membrane. It is part of a complex that includes stable interactions with other viral proteins necessary for replication. This interaction is key for ensuring viral propagation within if not outside its natural reservoirs and host organisms.
- Pathways
Reston virus glycoprotein facilitates entry into the host cell through the endocytosis pathway. It closely interacts with cellular receptors such as NPC1 affecting how the virus exploits host cellular machinery for entry and replication. Other viral proteins collaborate or compete in these pathways to enable the virus's lifecycle to proceed efficiently.
- Associated diseases and disorders
Reston virus glycoprotein associates with hemorrhagic fevers mainly when Reston virus crosses species barriers from natural hosts. While it has not caused significant disease in humans potential zoonotic transmission remains a concern. The glycoprotein's interaction with the immune regulatory protein TIM-1 may influence disease severity and progression in the host.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-REBOV GP antibody (ab190658)
REBOV GP is visualized as a broad band representing differing glycosylation patterns.
All lanes: Western blot - Anti-REBOV GP antibody (ab190658) at 0.05 µg/mL
Lane 1: REBOV rGPdTM supernatant at 15 µL
Lane 2: ZEBOV rGPdTM at 0.2 µg
Lane 3: SEBOV rGPdTM at 0.2 µg
Lane 4: MARV rGPdTM at 0.2 µg
Lane 5: BEBOV rGPdTM at 0.2 µg
Predicted band size: 42 kDa
Western blot - Anti-REBOV GP antibody (ab190658)
REBOV GP is visualized as a broad band representing differing glycosylation patterns.
All lanes: Western blot - Anti-REBOV GP antibody (ab190658) at 0.5 µg/mL
Lane 1: REBOV rGPdTM supernatant at 15 µL
Lane 2: ZEBOV rGPdTM at 0.2 µg
Lane 3: SEBOV rGPdTM at 0.2 µg
Lane 4: MARV rGPdTM at 0.2 µg
Lane 5: BEBOV rGPdTM at 0.2 µg
Predicted band size: 42 kDa
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