Anti-REC8 antibody [EPR16189]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (AB192241)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (AB192241)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (AB192241)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-REC8 antibody [EPR16189] (AB192241)

All lanes:

Western blot - Anti-REC8 antibody [EPR16189] (ab192241) at 1/1000 dilution

Lane 1:

Human testis lysate at 20 µg

Lane 2:

Jurkat lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 63 kDa

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• WB

Supplier Data

Western blot - Anti-REC8 antibody [EPR16189] (AB192241)

All lanes:

Western blot - Anti-REC8 antibody [EPR16189] (ab192241) at 1/10000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Rat testis lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 63 kDa

false

• WB

CiteAb

Western blot - Anti-REC8 antibody [EPR16189] (AB192241)

REC8 western blot using anti-REC8 antibody [EPR16189] ab192241. Publication image and figure legend from Mu, X., Wen, J., et al., 2018, Biol Open, PubMed 30420384.

ab192241 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab192241 please see the product overview.

CYP51 regulates the expression of the meiosis-specific cohesin subunits REC8 and STAG3. (A,B) RS21745 treatment decreased REC8 and STAG3 expression. Ovaries at 14.5 dpc were cultured with 10 μM RS21745 for 3 days in vitro. (A) Expression of the cohesin subunits following RS21745 treatment by qRT-PCR. All qRT-PCR values were normalized to β-actin and were expressed as a relative ratio to the control; the means±s.e.m. of 3 values are shown. (B) Expression of REC8 and STAG3 following RS21745 treatment by western blot GAPDH was used as internal reference. (C–F) Inhibition of CYP51 disturbed the distribution of both REC8 and STAG3 on the chromosomes. (C,E) In the control groups, REC8 and STAG3 expression was observed at high levels in zygotene oocytes. In the CYP51 inhibition groups, the cohesin signals were absent in zygotene cells. (D,F) The percentage of REC8-positive or STAG3-positive oocytes at the zygotene stage was recorded. Unidentified germ cells were not included in the analyses. Scale bars : 10 μm. The data are presented as the means±s.e.m. of 3–9 ovaries per group. Asterisk (*) denotes a statistically significant difference between the control and the treatment groups. ***p<0.001 (t-test).

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