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AB245771

Anti-REC8 antibody [EPR16189] - BSA and Azide free

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Rabbit Recombinant Monoclonal REC8 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

View Alternative Names

REC8L1, REC8, Meiotic recombination protein REC8 homolog, Cohesin Rec8p

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] - BSA and Azide free (AB245771)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] - BSA and Azide free (AB245771)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192241).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] - BSA and Azide free (AB245771)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] - BSA and Azide free (AB245771)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling REC8 with ab192241 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192241).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16189

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab245771 is the carrier-free version of ab192241.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein REC8 also known as Meiotic Recombination Protein REC8 Homolog plays a mechanical role in the cohesion of sister chromatids during meiosis. This protein weighs approximately 60 kDa and is expressed mostly in germ cells during the prophase of meiosis I. REC8 is structurally related to the cohesin complex family and its primary mechanical function is to maintain the physical connection between sister chromatids ensuring accurate chromosomal segregation during meiosis.
Biological function summary

Sister chromatid cohesion during meiosis depends heavily on REC8 which integrates into the cohesin protein complex. This complex consists of SMC1β SMC3 and STAG3 playing an essential role in meiotic chromosome pairing and segregation. REC8 forms part of this cohesin structure specifically ensuring precise cleavage and separation of chromatids during the anaphase I stage of meiosis which contributes critical fidelity in chromosomal inheritance.

Pathways

REC8 influences the meiotic cell cycle and the associated recombination pathways. These pathways are highly regulated and critical for gametogenesis. Within the meiotic cell cycle pathway REC8 interacts closely with the protein RAD21 which holds key roles across various stages of the cell cycle. The well-orchestrated removal of cohesion by separase enzyme ensures successful homologous chromosome separation highlighting REC8's importance in these cellular processes.

Defects in REC8 function show associations with infertility. Malfunctions in meiotic processes can lead to aneuploidies such as Down syndrome due to improper segregation of chromosomes. At the molecular level mutations or deficiencies in REC8 also connect to cohesinopathies like Cornelia de Lange syndrome where cohesion factor SMC3 could also play a part suggesting shared pathways or compensatory mechanisms related to chromosomal cohesion and segregation during meiosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).
See full target information REC8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com