AB63797

Anti-RecA antibody

Name: Anti-RecA antibody (ab63797) | Abcam
Brand: Abcam Limited
SKU: AB63797
Price: 450 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(44 Publications)

Rabbit Polyclonal RECA antibody. Suitable for WB, IP and reacts with Escherichia coli samples. Cited in 44 publications.

View Alternative Names

lexB, recH, rnmB, tif, umuB, zab, b2699, JW2669, recA, Protein RecA, Recombinase A

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Supplier Data

Immunoprecipitation - Anti-RecA antibody (AB63797)

Immunoprecipitation of RecA protein from crude extract of E. coli cells. 2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

All lanes:

Immunoprecipitation - Anti-RecA antibody (ab63797)

Predicted band size: 36 kDa

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Western blot - Anti-RecA antibody (AB63797)

All lanes:

Western blot - Anti-RecA antibody (ab63797) at 1/3000 dilution

Lane 1:

Molecular weight markers

Lane 2:

Purified RecA

Lane 3:

Extract of E.coli ab1157

Predicted band size: 36 kDa

Observed band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Escherichia coli

Applications

WB, IP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/600", "IP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein RecA also known as protein recA My RecA or BB RecA plays a significant role in DNA repair and homologous recombination. RecA has a molecular mass of approximately 38 kilodaltons and is highly conserved among various species including in bacteria E. coli. It features ATPase activity allowing it to bind and hydrolyze ATP to facilitate its functions. RecA is expressed widely in bacterial cells where it forms a filament with single-stranded DNA to initiate repair processes.

Biological function summary

RecA performs several critical functions in maintaining genetic stability. It facilitates homologous pairing and strand exchange essential during DNA repair and recombination events. RecA operates as part of a nucleoprotein filament a complex formed during its interaction with single-stranded DNA. Additionally it activates the autocatalytic cleavage of the LexA repressor which induces the SOS response in bacteria following DNA damage. This response includes the upregulation of various DNA repair enzymes contributing to cell survival under stress conditions.

Pathways

RecA integrates into cellular processes like the SOS response and homologous recombination. In the SOS response pathway it regulates the cleavage of the LexA repressor protein resulting in the induction of DNA repair genes. In homologous recombination RecA mediates the search for homologous sequences and promotes strand invasion leading to genetic exchange. RecA interacts with proteins such as RuvA and RuvB which assist in branch migration and resolution of recombination intermediates.

RecA-related functions can provide insights into bacterial pathogenicity and stress resistance. Deficiencies in RecA function may result in increased susceptibility to DNA damage affecting bacteria's ability to survive under environmental stresses. While not directly linked to human diseases understanding RecA's mechanisms informs the development of antimicrobial strategies targeting bacterial resistance. Proteins involved in similar repair pathways such as Rad51 in eukaryotes offer parallels for comparing DNA repair processes across different organisms and help in understanding bacterial resilience and persistence.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed : 22412352).. Mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy).

See full target information recA

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003260 PubMed40694573

2025

Systematic screen uncovers regulator contributions to chemical cues in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Binsfeld,Roberto Olayo-Alarcon,Lucía Pérez Jiménez,Morgane Wartel,Mara Stadler,André Mateus,Christian Müller,Ana Rita Brochado

PLoS pathogens 21:e1013151 PubMed40327678

2025

Origin, evolution, and success of pbla, the gonococcal beta-lactamase plasmid, and implications for public health.

Applications

Unspecified application

Species

Unspecified reactive species

Tabea A Elsener,Ana Cehovin,Connor Philp,Kate Fortney,Stanely M Spinola,Martin C J Maiden,Christoph M Tang

Nature microbiology 9:405-420 PubMed38316932

2024

Stepwise assembly and release of Tc toxins from Yersinia entomophaga.

Applications

Unspecified application

Species

Unspecified reactive species

Miki Feldmüller,Charles F Ericson,Pavel Afanasyev,Yun-Wei Lien,Gregor L Weiss,Florian Wollweber,Marion Schoof,Mark Hurst,Martin Pilhofer

PLoS biology 21:e3002198 PubMed37594988

2023

A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Mitosch,Martin Beyß,Prasad Phapale,Bernhard Drotleff,Katharina Nöh,Theodore Alexandrov,Kiran R Patil,Athanasios Typas

International journal of molecular sciences 24: PubMed37511505

2023

Exploring the Potential of a Genome-Reduced Strain for Plasmid DNA Production.

Applications

Unspecified application

Species

Unspecified reactive species

Thi Thuy Nguyen,Le Minh Bui,Ji-Young Byun,Byung-Kwan Cho,Sun Chang Kim

Nucleic acids research 51:5527-5546 PubMed37070184

2023

RecA and SSB genome-wide distribution in ssDNA gaps and ends in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong Pham,Elizabeth A Wood,Michael M Cox,Myron F Goodman

Microbiology spectrum 10:e0067822 PubMed36169422

2022

Metabolic and Morphotypic Trade-Offs within the Eco-Evolutionary Dynamics of Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Zlatkov,Moa Elsa Cecilia Näsman,Bernt Eric Uhlin

PLoS genetics 18:e1010238 PubMed35653392

2022

Single strand gap repair: The presynaptic phase plays a pivotal role in modulating lesion tolerance pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Luisa Laureti,Lara Lee,Gaëlle Philippin,Michel Kahi,Vincent Pagès

Nature microbiology 7:397-410 PubMed35165385

2022

Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Xu,Charles F Ericson,Yun-Wei Lien,Florentine U N Rutaganira,Fabian Eisenstein,Miki Feldmüller,Nicole King,Martin Pilhofer

PLoS genetics 17:e1009717 PubMed34432790

2021

Distribution of Holliday junctions and repair forks during Escherichia coli DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Tahirah Yasmin,Benura Azeroglu,Charlotte A Cockram,David R F Leach

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RecA antibody

Immunoprecipitation - Anti-RecA antibody (AB63797)

Western blot - Anti-RecA antibody (AB63797)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (44)

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Secondary Antibodies

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