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AB88249

Anti-RECK antibody

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(5 Publications)

Mouse Polyclonal RECK antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RECK.

View Alternative Names

ST15, RECK, Reversion-inducing cysteine-rich protein with Kazal motifs, hRECK, Suppressor of tumorigenicity 15 protein

2 Images
Western blot - Anti-RECK antibody (AB88249)
  • WB

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Western blot - Anti-RECK antibody (AB88249)

All lanes:

Western blot - Anti-RECK antibody (ab88249) at 1 µg/mL

All lanes:

293 cell lysate at 50 µg

Predicted band size: 106 kDa

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Western blot - Anti-RECK antibody (AB88249)
  • WB

Unknown

Western blot - Anti-RECK antibody (AB88249)

All lanes:

Western blot - Anti-RECK antibody (ab88249) at 1 µg/mL

Lane 1:

RECK transfected 293T cell line at 25 µg

Lane 2:

Non-transfected 293T cell line at 25 µg

Predicted band size: 106 kDa

Observed band size: 106 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RECK.

O95980

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RECK also known as Reversion-inducing-cysteine-rich protein with kazal motifs is a 110 kDa membrane-anchored glycoprotein. It plays a role in inhibiting matrix metalloproteinases (MMPs) like MMP-2 and MMP-9 which are involved in extracellular matrix remodeling. RECK is widely expressed in various tissues including the brain heart and muscles. It interacts with other membrane proteins to modulate different cellular functions playing a part in maintaining the structure of tissues.
Biological function summary

RECK supports angiogenesis and tissue integrity by controlling matrix degradation. It is essential for proper vascular development. Although RECK is not part of a bigger complex it exerts its function by partnering with integrins and other membrane components. This interaction helps regulate cell migration and adhesion which are significant for normal development and wound healing processes.

Pathways

RECK engages in the regulation of the extracellular matrix and signaling cascades. It aligns with critical pathways like the Wnt and Rho GTPase pathways. These pathways are important in controlling cellular proliferation and movement. RECK's interaction with proteins like β-catenin and Ras further illustrates its importance in modulating cellular pathways connected to growth and movement.

RECK can affect cancer progression and metastasis. Its downregulation or malfunction is associated with increased tumor invasion and angiogenesis due to uncontrolled MMP activity. RECK's connection with MMP-2 and MMP-9 is significant here as these proteins promote tumor metastasis. Its role is also evident in neurological disorders like cerebral ischemia where defective matrix remodeling can exacerbate tissue damage. Researchers focus on RECK to develop strategies for cancer therapeutics and neuroprotective treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed : 28289266, PubMed : 30026314). Plays a key role in Wnt7-specific responses : required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands : acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (PubMed : 30026314). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed : 30026314). Also acts as a serine protease inhibitor : negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed : 18194466, PubMed : 9789069). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed : 9789069).
See full target information RECK
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Archives of pharmacal research 45:475-493 PubMed35767208

2022

Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Noha F Abdelkader,Marawan A Elbaset,Passant E Moustafa,Sherehan M Ibrahim

OncoTargets and therapy 12:2323-2333 PubMed30992669

2019

MicroRNA-221 promotes papillary thyroid carcinoma cell migration and invasion via targeting RECK and regulating epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Li Wei,Ai-Bin Gao,Qing Wang,Xiu-E Lou,Jing Zhao,Qing-Jun Lu

Anticancer research 38:2031-2035 PubMed29599320

2018

MicroRNA-96 Promotes Tumor Invasion in Colorectal Cancer RECK.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhito Iseki,Masatsune Shibutani,Kiyoshi Maeda,Hisashi Nagahara,Tatsunari Fukuoka,Shinji Matsutani,Kosei Hirakawa,Masaichi Ohira

Medical science monitor : international medical jo 21:2718-25 PubMed26364844

2015

miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells.

Applications

WB

Species

Human

Wenneng Liu,Nian Song,Huihua Yao,Liying Zhao,Hao Liu,Guoxin Li

Molecular medicine reports 12:6031-6 PubMed26299812

2015

Epigallocatechin‑3‑gallate inhibits the invasion of salivary adenoid cystic carcinoma cells by reversing the hypermethylation status of the RECK gene.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qing Zhou,Xiao-Nan Xu,Lei Li,Jian-Jun Ma,En-Ming Zhen,Cheng-Bing Han
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB238162

Anti-RECK antibody

primary-antibodies

reck-antibody-ab238162

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