AB31928

Anti-Recoverin antibody [6A55CD6]

Name: Anti-Recoverin antibody [6A55CD6] (ab31928) | Abcam
Brand: Abcam Limited
SKU: AB31928
Price: 485 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(7 Publications)

Mouse Monoclonal Recoverin antibody. Suitable for WB, IHC-FoFr and reacts with Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

RCV1, RCVRN, Recoverin, Cancer-associated retinopathy protein, Protein CAR

2 Images

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Recoverin antibody [6A55CD6] (AB31928)

IHC-FoFr

Collaborator

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Recoverin antibody [6A55CD6] (AB31928)

ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.

This image is courtesy of an Abreview submitted by Dr Sophie Pezet

Lab

Western blot - Anti-Recoverin antibody [6A55CD6] (AB31928)

All lanes:

Western blot - Anti-Recoverin antibody [6A55CD6] (ab31928) at 5 µg/mL

Lane 1:

Mouse Retina Whole Cell Lysate at 10 µg

Lane 2:

Human Retina Normal Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 23 kDa

true

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A55CD6

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-FoFr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Recoverin also known as RCVRN is a calcium-binding protein part of the neuronal calcium sensor family. With a molecular weight of approximately 23 kDa it is mainly found in the retina and pineal gland. It functions in the phototransduction process particularly in the recovery phase of visual responses. Recoverin interacts with other proteins in a calcium-dependent manner and plays a role in inhibiting rhodopsin kinase activity when calcium levels are high.

Biological function summary

Recoverin regulates the inhibition of rhodopsin phosphorylation in photoreceptor cells. It is not part of a complex but works closely with G-protein coupled receptors in the retina to modulate the recovery of photo responses following light exposure. Its ability to bind to calcium ions triggers conformational changes that allow it to carry out these critical functions.

Pathways

One can consider that recoverin plays a significant role in the visual cycle and the phototransduction cascade. In these pathways recoverin modulates rhodopsin kinase and other related proteins to ensure proper rod and cone cell recovery following light stimulation. Recoverin interacts with proteins such as transducin enabling efficient modulation of light signal transduction and termination.

Recoverin has associations with cancer-associated retinopathy and age-related macular degeneration. Cancer-associated retinopathy an autoimmune condition involves antibodies against recoverin affecting normal function and leading to vision loss. In these conditions proteins like rhodopsin and other phototransduction components are impacted through the disruption caused by anti-recoverin antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells (By similarity). Modulates light sensitivity of cone photoreceptor in dark and dim conditions (By similarity). In response to high Ca(2+) levels induced by low light levels, prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting GRK1-mediated phosphorylation of RHO/rhodopsin (By similarity). Plays a role in scotopic vision/enhances vision in dim light by enhancing signal transfer between rod photoreceptors and rod bipolar cells (By similarity). Improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light (By similarity).

See full target information RCVRN

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 240:e31482 PubMed39605294

2024

Spontaneously Immortalised Nonhuman Primate Müller Glia Cell Lines as Source to Explore Retinal Reprogramming Mechanisms for Cell Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Salman,Arantxa Bolinches-Amorós,Tina Storm,Daniela Moralli,Paulina Bryika,Angela J Russell,Stephen G Davies,Alun R Barnard,Robert E MacLaren

PloS one 19:e0300584 PubMed38709779

2024

Genetic manipulation of rod-cone differences in mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Ala Morshedian,Zhichun Jiang,Roxana A Radu,Gordon L Fain,Alapakkam P Sampath

International journal of molecular sciences 23: PubMed36142120

2022

Regulates Subclinical Inflammation and Activates Proangiogenic Response in the Retina and Choroid.

Applications

Unspecified application

Species

Unspecified reactive species

Waseem Ahamed,Richard Ming Chuan Yu,Yang Pan,Takeshi Iwata,Veluchamy Amutha Barathi,Yeo Sia Wey,Sai Bo Bo Tun,Beiying Qiu,Alison Tan,Xiaomeng Wang,Chui Ming Gemmy Cheung,Tien Yin Wong,Yasuo Yanagi

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:2180-2189 PubMed35091503

2022

Reproducibility of the Rod Photoreceptor Response Depends Critically on the Concentration of the Phosphodiesterase Effector Enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Ala Morshedian,Gabriela Sendek,Sze Yin Ng,Kimberly Boyd,Roxana A Radu,Mingyao Liu,Nikolai O Artemyev,Alapakkam P Sampath,Gordon L Fain

International journal of molecular medicine 40:1172-1184 PubMed28848998

2017

Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Huiling Hu,Meixin Liang,Yunfan Xiong,Kang Li,Mengfei Chen,Zhigang Fan,Xielan Kuang,Fei Deng,Xiaohong Liu,Chaochao Xu,Kaijing Li,Jian Ge

Investigative ophthalmology & visual science 58:2306-2316 PubMed28431434

2017

Bright Light Suppresses Form-Deprivation Myopia Development With Activation of Dopamine D1 Receptor Signaling in the ON Pathway in Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Si Chen,Zhina Zhi,Qingqing Ruan,Qingxia Liu,Fen Li,Fen Wan,Peter S Reinach,Jiangfan Chen,Jia Qu,Xiangtian Zhou

Biomaterials 30:3405-14 PubMed19361860

2009

Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation.

Applications

ICC/IF

Species

Mouse

Stephen Redenti,William L Neeley,Santiago Rompani,Sunita Saigal,Jing Yang,Henry Klassen,Robert Langer,Michael J Young

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Recoverin antibody [6A55CD6]

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Recoverin antibody [6A55CD6] (AB31928)

Western blot - Anti-Recoverin antibody [6A55CD6] (AB31928)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise