AB151501

Anti-RECQ1 antibody

Name: Anti-RECQ1 antibody (ab151501) | Abcam
Brand: Abcam Limited
SKU: AB151501
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Polyclonal RECQ1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RECQL aa 400 to C-terminus.

View Alternative Names

RECQ1, RECQL1, RECQL, ATP-dependent DNA helicase Q1, DNA 3'-5' helicase Q1, DNA-dependent ATPase Q1, RecQ protein-like 1, RecQ1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (AB151501)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (AB151501)

Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RECQ1 with ab151501 at 1/100 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Unknown

Western blot - Anti-RECQ1 antibody (AB151501)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RECQ1 antibody (ab151501) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 73 kDa

false

Unknown

Western blot - Anti-RECQ1 antibody (AB151501)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RECQ1 antibody (ab151501) at 1/1000 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human RECQL aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46063

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Or perform heat mediated retrieval with Tris-EDTA buffer pH 8", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RECQ1 also known as RECQL or RECQL1 is a DNA helicase with a molecular mass around 73 kDa. It functions to unwind double-stranded DNA into single strands facilitating important processes like replication repair recombination and transcription. RECQ1 is expressed in various tissues with notable expression in rapidly dividing cells such as those in the thymus and testis. By unwinding DNA RECQ1 plays an important role in maintaining genomic stability and preventing DNA damage accumulation.

Biological function summary

RECQ1 influences genomic integrity by engaging in DNA repair processes. It is part of the RecQ helicase family which includes other helicases like BLM and WRN each with distinct roles in DNA metabolism. RECQ1 primarily participates in the base excision repair and homologous recombination repair pathways. It interacts with key proteins such as PARP1 and XRCC1 to fulfill its role in repairing DNA breaks thereby safeguarding the cell from genotoxic stress.

Pathways

RECQ1 significantly influences the homologous recombination and base excision repair pathways. In the homologous recombination pathway RECQ1 associates with proteins like RAD51 to facilitate the accurate repair of DNA double-strand breaks. In the base excision repair pathway RECQ1 works alongside APE1 to address single-strand breaks ensuring the continuity of the DNA replication process. These pathways are central to genomic integrity making RECQ1 a critical player in cellular responses to DNA damage.

RECQ1 plays a relevant role in certain cancers and premature aging syndromes. Its dysfunction can lead to chromosomal instability which may contribute to the development of various cancers including breast cancer. Additionally RECQ1's interaction with tumor suppressor proteins such as p53 further links it to cancer pathways and the cellular response to DNA damage. Mutations or diminished activity of RECQ1 have been associated with increased susceptibility to genomic disorders similar to those found in other RecQ helicase deficiencies such as Werner syndrome highlighting its importance in maintaining health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

DNA helicase that plays a role in DNA damage repair and genome stability (PubMed : 15886194, PubMed : 35025765, PubMed : 7527136, PubMed : 7961977, PubMed : 8056767). Exhibits a Mg(2+)- and ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed : 19151156, PubMed : 35025765, PubMed : 7527136, PubMed : 8056767). Full-length protein unwinds forked DNA substrates, resolves Holliday junctions, and has DNA strand annealing activity (PubMed : 19151156, PubMed : 25831490). Plays a role in restoring regressed replication forks (PubMed : 35025765). Required to restart stalled replication forks induced by abortive topoisomerase 1 and 2 lesions (PubMed : 35025765). Does not unwind G-quadruplex DNA (PubMed : 18426915). May play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens (PubMed : 15886194, PubMed : 7961977).

See full target information RECQL

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5143 PubMed37612308

2023

Trim33 masks a non-transcriptional function of E2f4 in replication fork progression.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Rousseau,Elias Einig,Chao Jin,Julia Horn,Mathias Riebold,Tanja Poth,Mohamed-Ali Jarboui,Michael Flentje,Nikita Popov

World journal of surgical oncology 21:231 PubMed37516854

2023

TRIM58 functions as a tumor suppressor in colorectal cancer by promoting RECQL4 ubiquitination to inhibit the AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Naizhi Sun,Jiacheng Shen,Yuhua Shi,Biao Liu,Shengguo Gao,Yichuan Chen,Jinwei Sun

The Journal of clinical investigation 132: PubMed35025765

2022

RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1.

Applications

Unspecified application

Species

Unspecified reactive species

Bassam Abu-Libdeh,Satpal S Jhujh,Srijita Dhar,Joshua A Sommers,Arindam Datta,Gabriel Mc Longo,Laura J Grange,John J Reynolds,Sophie L Cooke,Gavin S McNee,Robert Hollingworth,Beth L Woodward,Anil N Ganesh,Stephen J Smerdon,Claudia M Nicolae,Karina Durlacher-Betzer,Vered Molho-Pessach,Abdulsalam Abu-Libdeh,Vardiella Meiner,George-Lucian Moldovan,Vassilis Roukos,Tamar Harel,Robert M Brosh,Grant S Stewart

Nucleic acids research 49:11666-11689 PubMed34718742

2021

ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Bakr,Joschka Hey,Gianluca Sigismondo,Chun-Shan Liu,Ahmed Sadik,Ashish Goyal,Alice Cross,Ramya Lakshmana Iyer,Patrick Müller,Max Trauernicht,Kersten Breuer,Pavlo Lutsik,Christiane A Opitz,Jeroen Krijgsveld,Dieter Weichenhan,Christoph Plass,Odilia Popanda,Peter Schmezer

Cell reports 36:109688 PubMed34496242

2021

Endogenous Bos taurus RECQL is predominantly monomeric and more active than oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Na-Nv Liu,Ze-Yu Song,Hai-Lei Guo,Hu Yin,Wei-Fei Chen,Yang-Xue Dai,Ben-Ge Xin,Xia Ai,Lei Ji,Qing-Man Wang,Xi-Miao Hou,Shuo-Xing Dou,Stephane Rety,Xu-Guang Xi

Frontiers in cell and developmental biology 9:714868 PubMed34381789

2021

RECQ1 Promotes Stress Resistance and DNA Replication Progression Through PARP1 Signaling Pathway in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Hao Lian,Kui Chen,Ying Pang,Mu Chen,Bingsong Huang,Lei Zhu,Siyi Xu,Min Liu,Chunlong Zhong

Oncology letters 14:1807-1810 PubMed28789414

2017

miRNA-21 enhances chemoresistance to cisplatin in epithelial ovarian cancer by negatively regulating PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Yu,Yulong Chen,Ruiyun Tian,Jianxia Li,Hongyan Li,Teng Lv,Qin Yao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RECQ1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (AB151501)

Western blot - Anti-RECQ1 antibody (AB151501)

Western blot - Anti-RECQ1 antibody (AB151501)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise