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AB89817

Anti-RECQ1 antibody

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(3 Publications)

Mouse Polyclonal RECQ1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RECQL.

View Alternative Names

RECQ1, RECQL1, RECQL, ATP-dependent DNA helicase Q1, DNA 3'-5' helicase Q1, DNA-dependent ATPase Q1, RecQ protein-like 1, RecQ1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody (AB89817)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody (AB89817)

ab89817 at 10μg/ml staining RECQ1 in PFA-fixed, permeabilized HeLa cells by immunofluorescence.

Western blot - Anti-RECQ1 antibody (AB89817)
  • WB

Unknown

Western blot - Anti-RECQ1 antibody (AB89817)

All lanes:

Western blot - Anti-RECQ1 antibody (ab89817) at 1 µg/mL

All lanes:

HeLa cells nuclear extracts at 50 µg

Predicted band size: 73 kDa

Observed band size: 73 kDa

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Western blot - Anti-RECQ1 antibody (AB89817)
  • WB

Unknown

Western blot - Anti-RECQ1 antibody (AB89817)

All lanes:

Western blot - Anti-RECQ1 antibody (ab89817) at 1 µg/mL

Lane 1:

RECQ1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Predicted band size: 73 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RECQL.

P46063

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RECQ1 also known as RECQL or RECQL1 is a DNA helicase with a molecular mass around 73 kDa. It functions to unwind double-stranded DNA into single strands facilitating important processes like replication repair recombination and transcription. RECQ1 is expressed in various tissues with notable expression in rapidly dividing cells such as those in the thymus and testis. By unwinding DNA RECQ1 plays an important role in maintaining genomic stability and preventing DNA damage accumulation.
Biological function summary

RECQ1 influences genomic integrity by engaging in DNA repair processes. It is part of the RecQ helicase family which includes other helicases like BLM and WRN each with distinct roles in DNA metabolism. RECQ1 primarily participates in the base excision repair and homologous recombination repair pathways. It interacts with key proteins such as PARP1 and XRCC1 to fulfill its role in repairing DNA breaks thereby safeguarding the cell from genotoxic stress.

Pathways

RECQ1 significantly influences the homologous recombination and base excision repair pathways. In the homologous recombination pathway RECQ1 associates with proteins like RAD51 to facilitate the accurate repair of DNA double-strand breaks. In the base excision repair pathway RECQ1 works alongside APE1 to address single-strand breaks ensuring the continuity of the DNA replication process. These pathways are central to genomic integrity making RECQ1 a critical player in cellular responses to DNA damage.

RECQ1 plays a relevant role in certain cancers and premature aging syndromes. Its dysfunction can lead to chromosomal instability which may contribute to the development of various cancers including breast cancer. Additionally RECQ1's interaction with tumor suppressor proteins such as p53 further links it to cancer pathways and the cellular response to DNA damage. Mutations or diminished activity of RECQ1 have been associated with increased susceptibility to genomic disorders similar to those found in other RecQ helicase deficiencies such as Werner syndrome highlighting its importance in maintaining health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA helicase that plays a role in DNA damage repair and genome stability (PubMed : 15886194, PubMed : 35025765, PubMed : 7527136, PubMed : 7961977, PubMed : 8056767). Exhibits a Mg(2+)- and ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed : 19151156, PubMed : 35025765, PubMed : 7527136, PubMed : 8056767). Full-length protein unwinds forked DNA substrates, resolves Holliday junctions, and has DNA strand annealing activity (PubMed : 19151156, PubMed : 25831490). Plays a role in restoring regressed replication forks (PubMed : 35025765). Required to restart stalled replication forks induced by abortive topoisomerase 1 and 2 lesions (PubMed : 35025765). Does not unwind G-quadruplex DNA (PubMed : 18426915). May play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens (PubMed : 15886194, PubMed : 7961977).
See full target information RECQL
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Fungal biology and biotechnology 7:12 PubMed32765896

2020

Application of the human estrogen receptor within a synthetic transcription factor in .

Applications

Unspecified application

Species

Unspecified reactive species

Christian Derntl,Robert Mach,Astrid Mach-Aigner

Biotechnology for biofuels 12:231 PubMed31583017

2019

Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in .

Applications

Unspecified application

Species

Unspecified reactive species

Christian Derntl,Robert L Mach,Astrid R Mach-Aigner

Oncology reports 41:178-190 PubMed30320358

2018

Hypoxia increases chemoresistance in human medulloblastoma DAOY cells via hypoxia‑inducible factor 1α‑mediated downregulation of the CYP2B6, CYP3A4 and CYP3A5 enzymes and inhibition of cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús Valencia-Cervantes,Sara Huerta-Yepez,Guillermo Aquino-Jarquín,Sara Rodríguez-Enríquez,Daniel Martínez-Fong,José-Antonio Arias-Montaño,Víctor Manuel Dávila-Borja
View all publications
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Product promise

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