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AB224135

Anti-RECQL5 antibody

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(2 Publications)

Rabbit Polyclonal RECQL5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RECQL5 aa 550 to C-terminus.

View Alternative Names

RECQ5, RECQL5, ATP-dependent DNA helicase Q5, DNA 3'-5' helicase RecQ5, RecQ protein-like 5, RecQ5

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (AB224135)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (AB224135)

Paraffin-embedded human testis tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (AB224135)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (AB224135)

Paraffin-embedded human cervix tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (AB224135)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (AB224135)

Paraffin-embedded human cerebral cortex tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RECQL5 antibody (AB224135)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RECQL5 antibody (AB224135)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RECQL5 (green) using ab224135 at 4 μg/ml in ICC/IF.

Western blot - Anti-RECQL5 antibody (AB224135)
  • WB

Supplier Data

Western blot - Anti-RECQL5 antibody (AB224135)

All lanes:

Western blot - Anti-RECQL5 antibody (ab224135) at 1/100 dilution

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 109 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RECQL5 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RECQL5 which is also known as RECQL5 helicase functions as a DNA helicase. It plays roles in unwinding DNAn important for processes like replication repair and recombination. This protein has a molecular mass of approximately 99 kDa. Expression of RECQL5 occurs in various tissues with higher levels found in the brain and testis. Its helicase activity helps maintain genomic stability by resolving DNA structures during cellular replication processes.
Biological function summary

RECQL5 participates in maintaining genome stability by unwinding DNA strands. It does not merely act on its own; RECQL5 is part of complexes involved in DNA repair pathways. This protein interacts with other repair proteins to facilitate proper DNA replication and recombination ensuring the prevention of deleterious mutations and chromosomal abnormalities. Such interactions stabilize genomic integrity which is important for normal cell function and preventing malignancies.

Pathways

RECQL5 is involved in important cellular processes. It plays a significant role in the homologous recombination repair and the non-homologous end joining pathways. In these pathways RECQL5 interacts with proteins like RAD51 and XRCC4 respectively. Through these interactions RECQL5 ensures the accurate repair of DNA double-strand breaks preventing genomic instability and associated disorders.

Mutations or dysregulation of RECQL5 have associations with cancer and Werner syndrome. In cancer cells altered RECQL5 expression can lead to uncontrolled cell division due to faulty DNA repair often involving interactions with tumor suppressor proteins like p53. In Werner syndrome characterized by premature aging RECQL5 and its interactions with WRN protein may affect normal DNA repair and replication processes. Understanding its dysfunction aids in comprehending these conditions' pathogenesis and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed : 20643585, PubMed : 22973052, PubMed : 28100692). Probably unwinds DNA in a 3'-5' direction (Probable) (PubMed : 28100692). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed : 20231364). Also associates with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed : 27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed : 22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed : 28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed : 23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed : 28100692).
See full target information RECQL5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7796 PubMed36528626

2022

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8 T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas C J Tan,Van Kelly,Xiaoyan Zou,David Wright,Tony Ly,Rose Zamoyska

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33431668

2021

ATRX and RECQ5 define distinct homologous recombination subpathways.

Applications

Unspecified application

Species

Unspecified reactive species

Amira Elbakry,Szilvia Juhász,Ki Choi Chan,Markus Löbrich
View all publications
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Product promise

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