Rabbit Polyclonal RED1 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ADARB1.
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- Molecular therapy. Nucleic acids 21:932-9532020Adenosine-to-Inosine Editing of Vasoactive MicroRNAs Alters Their Targetome and Function in Ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReginald V C T van der Kwast et. al.PubMed 32814251
- Nucleic acids research 46:7379-73952018SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaghuvaran Shanmugam,Fan Zhang,Harini Srinivasan,John Lalith Charles Richard,Kaiwen I Liu,Xiujun Zhang,Cheok Wei A Woo,Zi Hao M Chua,Jan Paul Buschdorf,Michael J Meaney,Meng How TanPubMed 29992293
- Nucleic acids research 46:7323-73382018Tissue-selective restriction of RNA editing of CaV1.3 by splicing factor SRSF9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Huang,Katannya Kapeli,Wenhao Jin,Yuk Peng Wong,Thiruma Valavan Arumugam,Joanne Huifen Koh,Sumitra Srimasorn,Karthik Mallilankaraman,John Jia En Chua,Gene W Yeo,Tuck Wah SoongPubMed 29733375
- Genes 9:2018Differential Enzymatic Activity of Rat ADAR2 Splicing Variants Is Due to Altered Capability to Interact with RNA in the Deaminase Domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlice Filippini,Daniela Bonini,Edoardo Giacopuzzi,Luca La Via,Fabrizio Gangemi,Marina Colombi,Alessandro BarbonPubMed 29419780
- Molecular cell 69:126-135.e62018N-Methyladenosines Modulate A-to-I RNA Editing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Feng Xiang,Qin Yang,Chu-Xiao Liu,Man Wu,Ling-Ling Chen,Li YangPubMed 29304330
- RNA biology 14:1580-15912017Absence of the Fragile X Mental Retardation Protein results in defects of RNA editing of neuronal mRNAs in mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlice Filippini,Daniela Bonini,Caroline Lacoux,Laura Pacini,Maria Zingariello,Laura Sancillo,Daniela Bosisio,Valentina Salvi,Jessica Mingardi,Luca La Via,Francesca Zalfa,Claudia Bagni,Alessandro BarbonPubMed 28640668
- PloS one 9:e912882014Down-regulation of the RNA editing enzyme ADAR2 contributes to RGC death in a mouse model of glaucoma.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADARB1. The exact immunogen used to generate this antibody is proprietary information. Database link P78563
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested |
| Mouse | Expected | Predicted | Expected | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis. Isoform 1. Has a lower catalytic activity than isoform 2. Isoform 2. Has a higher catalytic activity than isoform 1.
Alternative names
ADAR2, DRADA2, RED1, ADARB1, Double-stranded RNA-specific editase 1, RNA-editing deaminase 1, RNA-editing enzyme 1, dsRNA adenosine deaminase
Recommended products
Rabbit Polyclonal RED1 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ADARB1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADARB1. The exact immunogen used to generate this antibody is proprietary information. Database link P78563
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RED1 also known as ADARB1 is a protein involved in RNA editing. It weighs approximately 73 kDa. RED1 mainly expresses itself in the central nervous system but also appears in other tissues. This protein modifies RNA molecules by catalyzing the conversion of adenosine to inosine which can alter the coding potential and metabolism of the RNA it acts upon.
- Biological function summary
The protein RED1 functions within RNA editing complexes. It co-operates with other proteins like ADAR1 to carry out its editing role. In particular RED1 affects the regulation of gene expression influencing neuronal function and development in the brain. Through its editing capabilities the protein contributes to the diversity and stability of RNA transcripts.
- Pathways
RED1 plays a role in neurotransmission and neurodevelopment pathways. It interacts with other editing proteins in the ADAR family to ensure proper RNA sequence modifications critical for brain function. The editing process that RED1 participates in impacts synaptic transmission important for neuronal communication and neural network formation.
- Associated diseases and disorders
The protein RED1 is associated with neurological disorders. Changes in RED1 expression or mutations can link to conditions such as epilepsy and schizophrenia. Aberrations in RNA editing performed by RED1 might lead to the dysfunction of proteins involved in neuronal signaling. It also connects with proteins like ADAR2 influencing the pathological progress of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-RED1 antibody (ab64830)
All lanes: Western blot - Anti-RED1 antibody (ab64830) at 1/500 dilution
Lane 1: HepG2 cell extract (5-30 µg total protein)
Lane 2: HepG2 cell extract (5-30 µg total protein) ) with 5-10 µg of the immunising peptide
Predicted band size: 81 kDa
Observed band size: 81 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RED1 antibody (ab64830)
ICC/IF image of ab64830 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64830, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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