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Rabbit Recombinant Monoclonal RED2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (AB307893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] (AB307893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] (AB307893), expandable thumbnail
  • Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (AB307893), expandable thumbnail
  • Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (AB307893), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow CytIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Lacks editing activity. It prevents the binding of other ADAR enzymes to targets in vitro, and decreases the efficiency of these enzymes. Capable of binding to dsRNA but also to ssRNA (By similarity).

Additional Targets

ADARB1

Alternative names

Adar3, Red2, Adarb2, Double-stranded RNA-specific editase B2, RNA-dependent adenosine deaminase 3, RNA-editing deaminase 2, RNA-editing enzyme 2, dsRNA adenosine deaminase B2

Recommended products

Rabbit Recombinant Monoclonal RED2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26577-102
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RED1 and RED2 also known as RNA editing proteins are involved in the post-transcriptional modification of RNA molecules. RED1 has a molecular mass of approximately 60 kDa while RED2 weighs around 62 kDa. Both proteins are expressed in various tissues notably in the brain and liver where they function in RNA editing processes. These proteins participate actively in the editing of adenosine to inosine within RNA a critical step in processing RNA molecules for proper function.

Biological function summary

RED1 and RED2 play key roles in the regulation of gene expression by influencing the maturation and stability of RNA transcripts. Both proteins are integral components of the RNA editing complex working with other editing enzymes to make precise modifications. Through their activity they affect the function and diversity of proteins encoded by the modified RNA impacting cellular mRNA landscapes and influencing coding potential.

Pathways

RED1 and RED2 are significant contributors within the RNA-related regulatory pathways. Their editing activities are important in the RNA editing pathway impacting neuronal signaling and immune response pathways. These proteins interact with ADAR enzymes which help facilitate the conversion of adenosine to inosine in double-stranded RNA regions ensuring correct RNA sequence processing and function.

Associated diseases and disorders

RED1 and RED2 have associations with neurodegenerative diseases and certain cancers. Aberrant RNA editing patterns involving these proteins are linked to Alzheimer's disease where disrupted RNA splicing and regulation occur. Furthermore cancerous growths often show altered expression of RED1 and RED2 connecting them to dysregulated cellular proliferation. These proteins interact with other key regulatory proteins like ADAR1 which also influences similar pathological pathways in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893), expandable thumbnail

    Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    This antibody weakly detects RED1 when high levels of recombinant protein of RED1 is over-loaded in Western Blot. Please apply the antibody with an optimized dilution.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human RED2 expression vector containing a his tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human RED1 expression vector containing a his tag whole cell lysate at 4 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Exposure time: 59s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling RED2 with ab307893 at 1/2000 dilution (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Negative control: No staining on mouse spleen.
    The section was incubated with ab307893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling RED2 with ab307893 at 1/2000 dilution (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Positive staining on mouse hippocampus.
    The section was incubated with ab307893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893), expandable thumbnail

    Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: spleen (PMID:31355950).

    In Western blot, anti-H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.

    Exposure time: 103 seconds.

    All lanes: Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893) at 1/1000 dilution

    Lane 1: Mouse cerebral cortex tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa

    Observed band size: 80 kDa

    Exposure time: 103s

  • Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893), expandable thumbnail

    Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 3: Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (ab307893) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free ab307894)

    Lane 1: 293T (Human embryonic kidney epithelial cell) +OE-empty at 20 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) +OE-mouse RED2 at 20 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) +OE-mouse RED1 at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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