Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (AB307894)

This data was produced using ab307893 the same antibody clone but in a different buffer. Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling RED2 with ab307893 at 1/2000 dilution (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse hippocampus. The section was incubated with ab307893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (AB307894)

This data was produced using ab307893 the same antibody clone but in a different buffer. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling RED2 with ab307893 at 1/2000 dilution (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse spleen. The section was incubated with ab307893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (AB307894)

This data was produced using ab307893 the same antibody clone but in a different buffer.

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

This antibody weakly detects RED1 when high levels of recombinant protein of RED1 is over-loaded in Western Blot. Please apply the antibody with an optimized dilution.

In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (<a href='/en-us/products/primary-antibodies/red1-red2-antibody-epr26577-102-ab307893'>ab307893</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human RED2 expression vector containing a his tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human RED1 expression vector containing a his tag whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

true

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (AB307894)

This data was produced using ab307893 the same antibody clone but in a different buffer. Blocking/Dilution buffer : 5% NFDM/TBST. Negative control : spleen (PMID : 31355950). In Western blot, anti-H3 antibody (ab176842) loading control staining at 1/100000 dilution. Exposure time : 103 seconds.

All lanes:

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (<a href='/en-us/products/primary-antibodies/red1-red2-antibody-epr26577-102-ab307893'>ab307893</a>) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 103s

• WB

Lab

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (AB307894)

This data was produced using ab307893 the same antibody clone but in a different buffer. Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1 - 3:

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] (<a href='/en-us/products/primary-antibodies/red1-red2-antibody-epr26577-102-ab307893'>ab307893</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-RED1 + RED2 antibody [EPR26577-102] - BSA and Azide free (ab307894)

Lane 1:

293T (Human embryonic kidney epithelial cell) +OE-empty at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) +OE-mouse RED2 at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) +OE-mouse RED1 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 10s