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AB251095

Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal REDD-1/DDIT4 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

REDD1, RTP801, DDIT4, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1, REDD-1

2 Images
Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (AB251095)
  • WB

Lab

Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (AB251095)

This data was developed using the same antibody clone in a different buffer formulation (ab191871).

Lanes 1-4 : Merged signal (red and green). Green - ab191871 observed at 35 kDa. Red - loading control ab8245 observed at 36 kDa.

ab191871 Anti-REDD-1/DDIT4 antibody [EPR18716] was shown to specifically react with REDD-1/DDIT4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264834 (knockout cell lysate ab257409) was used. Wild-type and REDD-1/DDIT4 knockout samples were subjected to SDS-PAGE. ab191871 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] (<a href='/en-us/products/primary-antibodies/redd-1-ddit4-antibody-epr18716-ab191871'>ab191871</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

DDIT4 knockout HeLa cell lysate at 20 µg

Lane 3:

HeLa treated with 0.5mM CoCL2 for 6 hours cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 35 kDa

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Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (AB251095)
  • WB

Lab

Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (AB251095)

This data was developed using ab191871, the same antibody clone in a different buffer formulation.

Western blot : Anti-DDIT4 antibody [EPR18716] (ab191871) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab191871 was shown to bind specifically to DDIT4. A band was observed at 25-37 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in DDIT4 knockout cell line. To generate this image, wild-type and DDIT4 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] (<a href='/en-us/products/primary-antibodies/redd-1-ddit4-antibody-epr18716-ab191871'>ab191871</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

DDIT4 knockout HCT 116 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 25-37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18716

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab251095 is the carrier-free version of ab191871.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REDD-1 also known as DDIT4 (DNA Damage Inducible Transcript 4) is a protein involved in cellular response to stress particularly hypoxic conditions. It has a molecular weight of approximately 35 kDa. This protein is expressed in various tissues including the brain liver and muscle indicating its importance in different physiological environments. REDD-1 acts as a negative regulator of the mechanistic target of rapamycin (mTOR) signaling pathway which plays an important role in cell growth and survival.
Biological function summary

The role of REDD-1 involves its participation in cellular adaptation to stress by inhibiting mTORC1 a complex within the mTOR pathway. Through this mechanism it helps cells to survive in limiting conditions by adjusting metabolic and growth signals. REDD-1 is not part of a larger protein complex but interacts closely with components of the mTOR pathway. Its regulation ensures proper cellular function under stress making it an important point for controlling cell behavior.

Pathways

REDD-1 serves as a significant regulator in the mTOR signaling and cellular stress response pathways. REDD-1 inhibits mTORC1 activity facilitating the cellular adaptation under nutrient starvation and hypoxic conditions. This inhibition affects the phosphorylation of downstream targets like S6K1 and 4EBP1 which are important for protein synthesis. Moreover REDD-1's relationship with the AMP-activated protein kinase (AMPK) adds another layer of regulation in energy balance and metabolic control.

REDD-1's dysregulation links to cancer and neurodegenerative diseases. In cancer altered REDD-1 expression affects mTORC1 signaling which can lead to unregulated cell growth and proliferation. The protein is connected to hypoxia-inducible factor (HIF) which contributes to tumor growth in hypoxic environments. In neurodegenerative diseases improper stress responses involving REDD-1 may exacerbate neuronal damage. Understanding REDD-1's interactions with mTORC1 and HIF could offer insights into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
See full target information DDIT4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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