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Mouse Monoclonal CD19 antibody - conjugated to redFluor™ 710. Suitable for Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - redFluor™ 710 Anti-CD19 antibody [HIB19] (AB242261), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
redFluor™ 710
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
5 µL for 105-108 Cells
Notes

5 μL (0.125 μg): The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x105 to 1x108 cells.

Associated Products

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Target data

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126).

Alternative names

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

Recommended products

Mouse Monoclonal CD19 antibody - conjugated to redFluor™ 710. Suitable for Flow Cyt and reacts with Human samples.

Key facts

Isotype
IgG1
Conjugation
redFluor™ 710
Form
Liquid
Clonality
Monoclonal
Clone number
HIB19
Purification technique
Affinity purification
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

Associated diseases and disorders

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

Product promise

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1 product image

  • Flow Cytometry - redFluor™ 710 Anti-CD19 antibody [HIB19] (ab242261), expandable thumbnail

    Flow Cytometry - redFluor™ 710 Anti-CD19 antibody [HIB19] (ab242261)

    Flow cytometric analysis of human peripheral blood lymphocyte cells staining CD19 using 5 μL (0.125 μg) ab242261 (solid blue line) or 0.125 μg redFluor™ 710 mouse IgG1 isotype control (dotted black line).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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