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AB243296

redFluor™ 710 Anti-CD3 antibody [OKT3]

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(1 Publication)

Mouse Monoclonal CD3d antibody - conjugated to redFluor™ 710. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - redFluor™ 710 Anti-CD3 antibody [OKT3] (AB243296)
  • Flow Cyt

Supplier Data

Flow Cytometry - redFluor™ 710 Anti-CD3 antibody [OKT3] (AB243296)

Flow cytometric analysis of human peripheral blood lymphocytes stained for CD3 with 5 μL (1 μg) ab243296 (solid line) or 1 μg redFluor™ 710 Mouse IgG2a isotype control (dashed line).

  • Carrier free

    Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free

  • Unconjugated

    Anti-CD3 antibody [OKT3]

  • 660 APC

    APC Anti-CD3 epsilon antibody [OKT3]

  • 578 PE

    PE Anti-CD3 epsilon antibody [OKT3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OKT3

Isotype

IgG2a

Light chain type

kappa

Conjugation

redFluor™ 710

Excitation/Emission

Ex: 684nm, Em: 704nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p></p>" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 40:101833 PubMed39398537

2024

CEACAM1 increased the lymphangiogenesis through miR-423-5p and NF- kB in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yu,Wenke Cai,Tao Zhou,Bo Men,Shunqiong Chen,Dong Tu,Wei Guo,Jicui Wang,Feipeng Zhao,Yan Wang
View all publications
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Product promise

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