JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242256

redFluor™ 710 Anti-CD45RO antibody [UCHL1]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CD45 antibody - conjugated to redFluor™ 710. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Flow Cytometry - redFluor™ 710 Anti-CD45RO antibody [UCHL1] (AB242256)
  • Flow Cyt

Supplier Data

Flow Cytometry - redFluor™ 710 Anti-CD45RO antibody [UCHL1] (AB242256)

Flow Cytometric analysis of human peripheral blood lymphocytes labeling CD45RO with 5 μl (0.5 μg) ab242256 (solid line) or 0.5 μg redFluor™ 710 Mouse IgG2a isotype control (dashed line).

  • 660 APC

    APC Anti-CD45R0 antibody [UCHL1]

  • 519 FITC

    FITC Anti-CD45R0 antibody [UCHL1]

  • 578 PE

    PE Anti-CD45R0 antibody [UCHL1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD45RO antibody [UCHL1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD45RO antibody [UCHL1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHL1

Isotype

IgG2a

Light chain type

kappa

Conjugation

redFluor™ 710

Excitation/Emission

Ex: 684nm, Em: 704nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

style
left-column

Alternative Products

Primary Antibodies

AB210385

FITC Anti-CD45RO antibody [UCHL1]

Flow Cytometry - FITC Anti-CD45RO antibody [UCHL1] (AB210385)

  • 0
  • 0
Primary Antibodies

AB216024

Anti-CD45RO antibody [T200/797]

  • 5
  • 1
Primary Antibodies

AB23

Anti-CD45RO antibody [UCHL1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCHL1] (AB23)

  • 5
  • 3
Primary Antibodies

AB77217

PE Anti-CD45RO antibody [UCHL1]

Flow Cytometry - PE Anti-CD45RO antibody [UCHL1] (AB77217)

  • 0
  • 0

Complementary Products

Assay Kits

AB277445

Mouse CD45 ELISA kit

ELISA - Mouse CD45 ELISA kit (AB277445)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1
Primary Antibodies

AB314277

PE Anti-CD105 antibody [EPR22811-18]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD105 antibody [EPR22811-18] (AB314277)

  • 0
  • 0
Primary Antibodies

AB282789

PE Anti-CD73 antibody [EPR23048-139]

RabMAb|Recombinant

Flow Cytometry - PE Anti-CD73 antibody [EPR23048-139] (AB282789)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>The antibody has been diluted for use at 5 μl per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl. The number of cells within a sample should be determined empirically, but typically ranges between 1 x 10e5 to 1 x 10e8 cells.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS via affinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RO also known as leukocyte common antigen 2 is an important isoform of the protein tyrosine phosphatase receptor type C (PTPRC). This protein has a molecular mass of about 180 kDa. Researchers find CD45RO expressed mostly on memory T cells a subset of T lymphocytes. These cells play an essential role in the immune system by remembering past infections or vaccinations. CD45RO functions mechanistically by modulating T cell receptor (TCR) signaling which is important for antibody production.
Biological function summary

This isoform plays an important role in immune responses by supporting the activation and differentiation of T cells. It is not part of a larger protein complex itself but interacts with other immune cell surface proteins for effective signaling. CD45RO is particularly important in the modulation of signal transduction through its phosphatase activity which removes phosphate groups from specific tyrosine residues on proteins influencing T cell sensitivity and response to antigens.

Pathways

CD45RO influences the JAK/STAT pathway a critical signaling pathway for cytokine receptor signaling in immune cells. It also plays a role in the TCR signaling pathway which is responsible for T cell activation. Within these pathways CD45RO acts in conjunction with other proteins like Lck and ZAP-70 that are also integral parts of T cell activation and proliferation processes essential for adaptive immune responses.

CD45RO is associated with autoimmune disorders such as rheumatoid arthritis where memory T cells play a role in sustaining inflammation. CD45RO also relates to chronic hepatitis B infection as it affects the disease progression through memory T cell-mediated immune responses. In these contexts CD45RO's interaction with other proteins like HLA-DR which is another immune-related surface protein becomes significant as it helps modulate immune responses that either suppress or exacerbate the disease symptoms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 17:173 PubMed31881947

2019

The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yu,Xueshibojie Liu,Guanghong Han,Yan Liu,Xue Zhao,Di Wang,Xiaomin Bian,Tingting Gu,Lianji Wen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com