AB242065

redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]

Name: Anti-Ly6g redFluor™ 710 antibody [RB6-8C5] (ab242065) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB242065
Price: 520 USD
Availability: InStock

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(1 Publication)

Rat Monoclonal Ly6g antibody - conjugated to redFluor™ 710. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

2 Images

Flow Cytometry - redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (AB242065)

Flow Cyt

Supplier Data

Flow Cytometry - redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (AB242065)

Flow cytometric analysis of C57Bl/6 bone marrow cells labeling Ly6g with 0.015 μg ab242065 (right panel) compared with a rat Isotype control (left panel).

Flow Cyt

Supplier Data

Flow Cytometry - redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (AB242065)

Flow cytometric analysis of C57Bl/6 bone marrow cells labeling Ly6g with 0.015 μg ab242065 (left panel) compared with an AlexaFluor^® 700-conjugated version of the same antibody clone (right panel).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RB6-8C5

Isotype

IgG2b

Light chain type

kappa

Conjugation

redFluor™ 710

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ly6g

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 16:867-883 PubMed37915320

2023

B7 Induces Apoptosis in Colorectal Cancer Cells by Regulating the Expression of Caspase-3 and Inhibits Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Zhang,Fengxi Li,Rong Li,Nan Zhao,Dianfeng Liu,Yuelin Xu,Lei Wang,Dongxu Wang,Ruihong Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]

Flow Cytometry - redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (AB242065)

Flow Cytometry - redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (AB242065)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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