Anti-Reelin antibody [EPR26278-30] 20 ul size (ab312310)

Rabbit Recombinant Monoclonal Reelin antibody. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Reelin antibody [EPR26278-30] (AB312310), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)	ICC/IF	IP
Human	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Tested	Tested	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes 0.515 ug/ml
Species Mouse	Dilution info 1/1000	Notes 0.515 ug/ml
Species Rat	Dilution info 1/1000	Notes 0.515 ug/ml

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Rat	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information RELN

Function

Extracellular matrix serine protease secreted by pioneer neurons that plays a role in layering of neurons in the cerebral cortex and cerebellum by coordinating cell positioning during neurodevelopment. Regulates microtubule function in neurons and neuronal migration. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion.

Alternative names

Reelin, RELN

Recommended products

Rabbit Recombinant Monoclonal Reelin antibody. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26278-30
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary: Reelin a large glycoprotein with a mass of approximately 388 kDa plays an integral role in brain development and function. Known for its presence in the extracellular matrix it is frequently referred to as RELN protein. Reelin is predominantly expressed in the brain especially in regions such as the cerebral cortex hippocampus and cerebellum. Its expression is highly regulated suggesting its importance in neurological processes.
Biological function summary: Reelin functions in the regulation of neuronal migration and positioning in the developing brain. Acting as a signaling molecule it forms part of a larger complex essential for proper neuronal layering and synaptic plasticity. Reelin interacts with various receptors on the cell surface initiating a cascade of intracellular signaling that influences cell orientation and dendritic growth.
Pathways: Reelin engages both the Reelin signaling pathway and the PI3K-Akt pathway highlighting its diverse influence on cellular processes. In the Reelin signaling pathway it associates with proteins like Dab1 and VLDLR playing an important role in the modulation of tyrosine kinases. This association affects neuronal structures by guiding their architectural development while interactions with the PI3K-Akt pathway impact cell survival and growth.
Associated diseases and disorders: Reelin has been implicated in conditions such as lissencephaly and autism spectrum disorders. Altered Reelin signaling may contribute to the abnormal neuronal migration seen in these diseases. Moreover its interplay with other proteins like ApoE which modifies brain structure and function in these disorders highlights Reelin’s relevance in maintaining neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

Western blot - Anti-Reelin antibody [EPR26278-30] (ab312310)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle.

In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 37 seconds
All lanes: Western blot - Anti-Reelin antibody [EPR26278-30] (ab312310) at 1/1000 dilution
Lane 1: Rat cerebellum tissue lysate at 20 µg
Lane 2: Rat hippocampus tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 420 kDa
Exposure time: 37s
Western blot - Anti-Reelin antibody [EPR26278-30] (ab312310)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: U-937; skeletal muscle.

In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 103 seconds
All lanes: Western blot - Anti-Reelin antibody [EPR26278-30] (ab312310) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20 at 20 µg
Lane 2: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg
Lane 4: Mouse P0 hippocampus tissue lysate at 20 µg
Lane 5: Mouse skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 420 kDa
Exposure time: 103s
Immunocytochemistry/ Immunofluorescence - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse hippocampus primary neuron cells labelling Reelin with ab312310 at 1/50 (10.3 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse hippocampus primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projectionImage was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized rat hippocampal neuron cells labelling Reelin with ab312310 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse cortex primary neuron cells labelling Reelin with ab312310 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling Reelin with ab312310 at 1/100 (5.15 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab312310 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat p0 hippocampus (fresh) tissue labeling Reelin with ab312310 at 1/100 (5.15 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat p0 hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab312310 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling Reelin with ab312310 at 1/100 (5.15 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab312310 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse p0 hippocampus (fresh) tissue labeling Reelin with ab312310 at 1/100 (5.15 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse p0 hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab312310 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling Reelin with ab312310 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse skeletal muscle.The section was incubated with ab312310 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Reelin with ab312310 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat liver hepatic stellate cells.The section was incubated with ab312310 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Reelin with ab312310 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse liver hepatic stellate cells.The section was incubated with ab312310 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Reelin with ab312310 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on a subset of neurons on rat cerebrum (PMID: 9501244).The section was incubated with ab312310 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Reelin with ab312310 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on a subset of neurons on mouse cerebrum (PMID: 20610758).The section was incubated with ab312310 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR26278-30] (ab312310)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Reelin with ab312310 at 1/500 (1.03 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on a subset of neurons on human cerebrum (PMID: 9861036).The section was incubated with ab312310 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins