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AB241964

Anti-REEP3 antibody

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(1 Review)

|

(2 Publications)

Rabbit Polyclonal REEP3 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human REEP3 aa 200-300.

View Alternative Names

C10orf74, REEP3, Receptor expression-enhancing protein 3

1 Images
Immunoprecipitation - Anti-REEP3 antibody (AB241964)
  • IP

Supplier Data

Immunoprecipitation - Anti-REEP3 antibody (AB241964)

REEP3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab241964 used for IP at 8 μl per reaction. For WB at a 1 : 400 dilution.

Lane 1 : ab241964 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-REEP3 antibody (ab241964)

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human REEP3 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q6NUK4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to REEP3 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REEP3 also known as Receptor Accessory Protein 3 plays an important role in mechanical processes within the cell. This protein assists in shaping the endoplasmic reticulum (ER) by facilitating the stacking and proper organization of the ER membranes. REEP3 has a molecular weight of approximately 28 kDa. Its expression is widespread but shows significant presence in neuronal tissues suggesting a specialized role in these cells.
Biological function summary

REEP3 manages the structural integrity of the endoplasmic reticulum by interacting with other proteins involved in ER morphology. It forms part of a complex that includes microtubule-associated proteins which link the ER to the cytoskeleton enabling proper cellular function. Through this interaction REEP3 supports the maintenance of cellular homeostasis and can influence processes such as protein synthesis and transport.

Pathways

Proteins like REEP3 contribute to ER shaping pathways and cellular stress response mechanisms. It acts alongside proteins like ATLASTIN and SEIPIN in the ER network assembly. Specifically it connects with pathways managing ER stress responses which are vital in adapting to and regulating changes in protein folding demand.

Aberrations in REEP3 function associate with conditions affecting the nervous system such as hereditary spastic paraplegia (HSP). Mutations in REEP3 can lead to defects in ER morphology contributing to neurodegenerative processes. The protein interacts with SPG4 and REEP1 linking it to cytoskeletal abnormalities observed in these disorders. Understanding REEP3's precise role in these pathologies offers potential avenues for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. Probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes.
See full target information REEP3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105160 PubMed35233964

2022

circREEP3 Drives Colorectal Cancer Progression via Activation of FKBP10 Transcription and Restriction of Antitumor Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Chen,Luyun He,Liangbo Zhao,Guangtan Zhang,Zhiwei Wang,Pingping Zhu,Benyu Liu

The Journal of clinical investigation 131: PubMed33079728

2020

Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Huang,Kenneth E Huffman,Zixi Wang,Xun Wang,Kailong Li,Feng Cai,Chendong Yang,Ling Cai,Terry S Shih,Lauren G Zacharias,Andrew Chung,Qian Yang,Milind D Chalishazar,Abbie S Ireland,C Allison Stewart,Kasey Cargill,Luc Girard,Yi Liu,Min Ni,Jian Xu,Xudong Wu,Hao Zhu,Benjamin Drapkin,Lauren A Byers,Trudy G Oliver,Adi F Gazdar,John D Minna,Ralph J DeBerardinis
View all publications
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Product promise

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