AB230115

Anti-REEP5 antibody

Name: Anti-REEP5 antibody (ab230115) | Abcam
Brand: Abcam Limited
SKU: AB230115
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal REEP5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human REEP5 aa 100 to C-terminus.

View Alternative Names

C5orf18, DP1, TB2, REEP5, Receptor expression-enhancing protein 5, Polyposis locus protein 1, Protein TB2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP5 antibody (AB230115)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP5 antibody (AB230115)

Paraffin-embedded human skin tissue stained for REEP5 using ab230115 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP5 antibody (AB230115)

Paraffin-embedded human colon cancer tissue stained for REEP5 using ab230115 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-REEP5 antibody (AB230115)

All lanes:

Western blot - Anti-REEP5 antibody (ab230115) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate

Lane 2:

Mouse lung tissue lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 5:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human REEP5 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q00765

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REEP5 also known as Receptor Expression Enhancing Protein 5 facilitates cell machinery in organizing and shaping the membranes of the endoplasmic reticulum. This protein has a molecular mass of approximately 22 kDa. REEP5 is expressed abundantly in the heart and skeletal muscle though it also exists in other tissues at lower levels. The protein is known to assist in membrane topology which is essential for maintaining proper endoplasmic reticulum morphology and function.

Biological function summary

REEP5 supports endoplasmic reticulum tubule formation helping maintain the high curvature structure of the organelle. It becomes part of a larger protein complex contributing to the development and maintenance of the fine network structure typical of the endoplasmic reticulum. By doing so REEP5 plays a role in the spatial organization of the cellular environment ensuring the proper segregation and function of cellular components.

Pathways

REEP5 actively participates in cellular stress response and the unfolded protein response pathway. This pathway involves proteins such as IRE1 and PERK which sensor and manage cellular stress by regulating protein folding and assisting in maintaining homeostasis. REEP5 provides structural regulation in these pathways impacting protein synthesis and secretion processes which are essential for adapting to cellular demands and stress.

REEP5 has been implicated in heart conditions particularly dilated cardiomyopathy. This disorder involves the heart muscle becoming weakened and enlarged affecting its ability to pump blood efficiently. Mutations or dysregulation of REEP5 impact its interaction with proteins like ATL1 and ATL2 which are involved in membrane fusion processes. Additionally REEP5 has shown associations with neurodegenerative conditions indicating its wider significance in maintaining cellular health and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an essential role in heart function and development by regulating the organization and function of the sarcoplasmic reticulum in cardiomyocytes.

See full target information REEP5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 38:549-557 PubMed36269039

2022

MiR-29c-3p represses gastric cancer development via modulating MEST.

Applications

Unspecified application

Species

Unspecified reactive species

Honghai Li,Jieqing Lv,Jindao Wang,Haifeng Wang,Liang Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-REEP5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP5 antibody (AB230115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP5 antibody (AB230115)

Western blot - Anti-REEP5 antibody (AB230115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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