AB204341

Anti-REEP6 antibody

Name: Anti-REEP6 antibody (ab204341) | Abcam
Brand: Abcam Limited
SKU: AB204341

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(1 Publication)

Rabbit Polyclonal REEP6 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human REEP6 aa 1-50.

View Alternative Names

C19orf32, DP1L1, REEP6, Receptor expression-enhancing protein 6, Polyposis locus protein 1-like 1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling REEP6 with ab204341 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-REEP6 antibody (AB204341)

Immunocytochemistry analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labelling REEP6 with ab204341 at 2 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling REEP6 with ab204341 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling REEP6 with ab204341 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling REEP6 with ab204341 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-REEP6 antibody (AB204341)

All lanes:

Western blot - Anti-REEP6 antibody (ab204341) at 1/500 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver tissue lysate

Predicted band size: 20 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human REEP6 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96HR9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REEP6 also known as Receptor Expression Enhancing Protein 6 is a protein with a molecular mass of approximately 27 kDa. Its main mechanical role is to assist with the proper folding and expression of receptor proteins in the endoplasmic reticulum (ER). REEP6 is strongly expressed in retinal cells but it may also be present in lower levels in other tissues. By interacting with newly synthesized proteins REEP6 helps in their stabilization and transport to the cell surface.

Biological function summary

The REEP6 protein supports sensory neuron function by regulating receptor proteins critical for processing sensory inputs. It does this as part of protein complexes within ER membranes where it influences the trafficking of G protein-coupled receptors (GPCRs) specifically involved in visual photoreception. In the context of the retina REEP6 actions enable photoreceptors to respond correctly to light which is important for vision.

Pathways

Research identifies REEP6 as integral to cellular mechanisms related to protein processing and trafficking pathways. It plays a role along with proteins such as GPrin1 and Sec24C in the phototransduction pathway which converts light stimuli into visual signals. These pathways control how proteins are sorted and delivered to their destinations affecting overall cell communication and homeostasis.

Mutations in REEP6 are linked to retinitis pigmentosa a degenerative eye condition leading to vision loss. This disorder affects photoreceptor function and is connected to rhodopsin protein deficiencies which are common in patients with this type of retinal disorder. Variants in REEP6 can also affect its function resulting in altered receptor expression that contributes to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Required for correct function and survival of retinal photoreceptors (PubMed : 27889058). Required for retinal development (By similarity). In rod photoreceptors, facilitates stability and/or trafficking of guanylate cyclases and is required to maintain endoplasmic reticulum and mitochondrial homeostasis (By similarity). May play a role in clathrin-coated intracellular vesicle trafficking of proteins from the endoplasmic reticulum to the retinal rod plasma membrane (By similarity).

See full target information REEP6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Burns & trauma 12:tkad058 PubMed38250706

2024

Exosomal miR-4645-5p from hypoxic bone marrow mesenchymal stem cells facilitates diabetic wound healing by restoring keratinocyte autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Shi,Shang Wang,Dewu Liu,Zhengguang Wang,Yihan Zhu,Jun Li,Kui Xu,Furong Li,Huicai Wen,Ronghua Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-REEP6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunocytochemistry - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REEP6 antibody (AB204341)

Western blot - Anti-REEP6 antibody (AB204341)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise