AB178440

Anti-Ref2P antibody

Name: Anti-Ref2P antibody (ab178440) | Abcam
Brand: Abcam Limited
SKU: AB178440
Price: 200 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(75 Publications)

Anti-Ref2P antibody (ab178440) is a rabbit polyclonal antibody detecting Ref2P in Western Blot. Suitable for Drosophila melanogaster.

- Over 40 publications

View Alternative Names

CG10360, ref(2)P, Protein ref(2)P, Refractory to sigma P

2 Images

Collaborator

Western blot - Anti-Ref2P antibody (AB178440)

Gel 8% acrylamide, transferred onto PVDF membrane for 60 minutes at 100V in 10% ethanol buffer. Anti-Ref2P antibody 1 : 500 incubated overnight at 4°C, with gentle agitation in TBS + 0.1% Tween-20.

The genotype of the Atg8a mutant flies used is : Atg8a^KG07569

All lanes:

Western blot - Anti-Ref2P antibody (ab178440) at 1/500 dilution

Lane 1:

Wt adult drosophila lysate at 20 µg

Lane 2:

Atg8a mutant adult drosophila lysate at 20 µg

Secondary

All lanes:

Anti-rabbit HRP at 1/10000 dilution

Predicted band size: 65 kDa

Observed band size: 100 kDa

false

Exposure time: 2s

This image is courtesy of Dr Anne-Claire Jacomin / Ioannis Nezis Lab, University of Warwick

Collaborator

Western blot - Anti-Ref2P antibody (AB178440)

The bands observed at the bottom of the gel represent Tubulin and were used as a loading control within this experiment.

All lanes:

Western blot - Anti-Ref2P antibody (ab178440) at 1/100 dilution

Lanes 1 - 3:

Atg8a mutant fruit flies (D.melanogaster)

Lanes 4 - 6:

Fruit flies (D.melanogaster) expressing ubiquitously Ref(2)p RNAi

Predicted band size: 65 kDa

Observed band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Ref2P antibody (ab178440) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Drosophila melanogaster samples.

What is the molecular weight of Ref2P?
Anti-Ref2P (ab178440) specifically detects a band for Ref2P (UniProt: P14199) at a molecular weight of 65kDa.

Trusted by the scientific community
Anti-Ref2P (ab178440) was first used in a scientific publication in 2013 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Ref2P (ab178440) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ref(2)P also known as Ref(2)P protein or Recycling Endosome Factor 2 Protein plays a significant role in autophagy in Drosophila. This protein is approximately 65 kDa in mass and is expressed widely in various tissues including neuronal and muscle tissues. Mechanically Ref(2)P functions as a scaffold protein facilitating the formation and degradation of protein aggregates. It also interacts with other key components of the autophagy machinery to promote the selective degradation of ubiquitinated proteins.

Biological function summary

This protein assists in maintaining cellular homeostasis by targeting damaged or unnecessary proteins for degradation. Ref(2)P serves as the fly homolog of the mammalian p62/SQSTM1 and is recognized as an adaptor protein within the autophagic machinery. It binds ubiquitinated proteins and interacts with LC3 a core component of the autophagosome aiding in the clearance of protein aggregates. This process is essential for protecting cells especially those under stress conditions.

Pathways

Ref(2)P integrates into the autophagy-lysosome pathway which is important for cellular recycling processes. It collaborates with key proteins like Atg1 and Atg8. Additionally the protein impacts the TOR signaling pathway. In a nutrient-rich environment TOR can inhibit autophagy while Ref(2)P acts in response to cellular stress promoting autophagy and improving metabolic homeostasis.

Abnormalities in Ref(2)P expression relate strongly to neurodegenerative diseases including conditions similar to Alzheimer's. Ref(2)P malfunctions can lead to the accumulation of protein aggregates which are harmful to neurons. This protein also connects indirectly to disorders involving the ubiquitin-proteasome system since it functions in tagging proteins for autophagic degradation. Therapeutic strategies targeting Ref(2)P or its pathways could potentially alleviate symptoms linked to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for selective autophagy activation by ubiquitinated proteins (PubMed : 22622177). Implicated in sigma rhabdovirus multiplication and necessary for male fertility. Involved in activating transcription of Drs (PubMed : 12446795, PubMed : 2510997).

See full target information ref(2)P

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Publications (75)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011818 PubMed40825051

2025

High-fat diet impairs intermediate-term memory by autophagic-lysosomal dysfunction in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Yue,Minrui Jiang,Kotomi Onuki,Motoyuki Itoh,Ayako Tonoki

Disease models & mechanisms 18: PubMed40747773

2025

HIV-1 Nef synergizes with APOL1-G1 to induce nephrocyte cell death in HIV-related kidney diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Yi Zhu,Yulong Fu,Joyce van de Leemput,Jing Yu,Jinliang Li,Patricio E Ray,Zhe Han

Autophagy reports 4:2519102 PubMed40607262

2025

Differential response of neurons to autophagy modulation in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ankit Sharma,Sushma Rao,Ravi Manjithaya,Vasu Sheeba

Nature communications 16:4909 PubMed40425608

2025

Restoring calcium crosstalk between ER and mitochondria promotes intestinal stem cell rejuvenation through autophagy in aged Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Zhang,Peng Ma,Saifei Wang,Shuxin Chen,Hansong Deng

Autophagy reports 4:2471121 PubMed40395995

2025

Loss of nuclear envelope bud formation leads to mitophagy initiation in muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Yungui Guo,David Brooks,Ziwei Zhao,Erica Biven,Erika R Geisbrecht

The Journal of cell biology 224: PubMed40067150

2025

The deubiquitinase USP45 inhibits autophagy through actin regulation by Coronin 1B.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchieh Jay Lin,Li-Ting Huang,Po-Yuan Ke,Guang-Chao Chen

Pharmaceutical biology 63:68-81 PubMed39862058

2025

A novel strategy for the protective effect of ginsenoside Rg1 against ovarian reserve decline by the PINK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pengdi Yang,Meiling Fan,Ying Chen,Dan Yang,Lu Zhai,Baoyu Fu,Lili Zhang,Yanping Wang,Rui Ma,Liwei Sun

Communications biology 8:105 PubMed39838082

2025

Dysfunctional BCAA degradation triggers neuronal damage through disrupted AMPK-mitochondrial axis due to enhanced PP2Ac interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Cheng Wu,Yan-Jhen Chen,Shih-Han Su,Pai-Hsiang Fang,Rei-Wen Liu,Hui-Ying Tsai,Yen-Jui Chang,Hsing-Han Li,Jian-Chiuan Li,Chun-Hong Chen

PLoS genetics 20:e1011475 PubMed39561115

2024

Neuronal fatty acid-binding protein enhances autophagy and suppresses amyloid-β pathology in a Drosophila model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Seokhui Jang,Byoungyun Choi,Chaejin Lim,Minkyoung Kim,Ji-Eun Lee,Hyungi Lee,Eunji Baek,Kyoung Sang Cho

Methods in molecular biology (Clifton, N.J.) 2879:23-32 PubMed39120738

2024

Monitoring Autophagy During Drosophila Oogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mrunmayee Kulkarni,Karan Selarka,Bhupendra V Shravage

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ref2P antibody

Western blot - Anti-Ref2P antibody (AB178440)

Western blot - Anti-Ref2P antibody (AB178440)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (75)

Product promise