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AB47099

Anti-REG1 alpha antibody

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(6 Publications)

Anti-REG1 alpha antibody (ab47099) is a rabbit polyclonal antibody detecting REG1 alpha in Western Blot. Suitable for Human.

- Trusted since 2007

View Alternative Names

PSPS, PSPS1, REG, REG1A, Lithostathine-1-alpha, Islet cells regeneration factor, Islet of Langerhans regenerating protein, Pancreatic stone protein, Pancreatic thread protein, Regenerating islet-derived protein 1-alpha, Regenerating protein I alpha, ICRF, PSP, PTP, REG-1-alpha

1 Images
Western blot - Anti-REG1 alpha antibody (AB47099)
  • WB

Unknown

Western blot - Anti-REG1 alpha antibody (AB47099)

The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.

All lanes:

Western blot - Anti-REG1 alpha antibody (ab47099) at 1 µg/mL

All lanes:

Human pancreas tissue lysate - total protein (<a href='/en-us/products/unavailable/human-pancreas-tissue-lysate-total-protein-ab29816'>ab29816</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human REG1A.

P05451

Specificity

This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity.

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Alternative Products

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AB231092

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG1 alpha antibody (AB231092)

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Complementary Products

Primary Antibodies

AB231092

Anti-REG1 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG1 alpha antibody (AB231092)

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Secondary Antibodies

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Anti-Iba1 antibody [EPR16588] - Microglia marker

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

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Primary Antibodies

AB168387

Anti-Aquaporin 1 antibody [EPR11588(B)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

  • 5
  • 5
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Reactivity data

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Product details

What is this antibody validated in?
Anti-REG1 alpha antibody (ab47099) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of REG1 alpha?
Anti-REG1 alpha (ab47099) specifically detects a band for REG1 alpha (UniProt: P05451) at a molecular weight of 19kDa.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha.
Storage buffer
Constituents: PBS, 0.58% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REG1 alpha also known as regenerating islet-derived protein 1 alpha or REG1A is a 19 kDa protein. Found primarily in the pancreas this protein also shows expression in gastric and intestinal tissues. REG1 alpha belongs to the C-type lectin superfamily known for their carbohydrate recognition capabilities. The protein involves itself directly in cell growth and proliferation through regenerative patterns essential for cellular homeostasis.
Biological function summary

The REG1 alpha protein stimulates pancreatic beta-cell proliferation influencing insulin production and secretion. It exists as a monomer but may participate in larger complexes during specific cellular responses. Its role expands to the modulation of cellular differentiation and inflammation control within the gastrointestinal tract. By aiding tissue repair REG1 alpha contributes to the maintenance of a healthy epithelial lining.

Pathways

The mitogen-activated protein kinase (MAPK) pathway sees REG1 alpha engaging in the regulation of cell proliferation by interacting with similar proteins involved in this signaling cascade. It also plays a role in the JAK-STAT pathway where it coordinates with the interleukins which are important for signaling that controls immune responses. REG1 alpha’s function in these pathways highlights its role in the regulation of growth and repair mechanisms.

REG1 alpha shows altered expression patterns in diabetes and some forms of cancer such as pancreatic cancer. In diabetes the protein’s involvement with glucose metabolism connects it to insulin regulation issues often interacting with insulin signaling proteins. In pancreatic cancer changes in REG1 alpha levels correlate with cellular growth and tissue regeneration processes that may become dysregulated further involving proteins like KRAS that stimulate cancer cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
See full target information REG1A
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:10943-10971 PubMed38944814

2024

Unfolding the mysteries of heterogeneity from a high-resolution perspective: integration analysis of single-cell multi-omics and spatial omics revealed functionally heterogeneous cancer cells in ccRCC.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zheng,Wenhao Lu,Chengbang Wang,Shaohua Chen,Qingyun Zhang,Cheng Su

International journal of molecular sciences 23: PubMed35955718

2022

Reg-1α, a New Substrate of Calpain-2 Depending on Its Glycosylation Status.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Christine Lebart,Françoise Trousse,Gilles Valette,Joan Torrent,Morgane Denus,Nadine Mestre-Frances,Anne Marcilhac

Frontiers in oncology 11:719564 PubMed34722263

2021

Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Su,Yufang Lv,Wenhao Lu,Zhenyuan Yu,Yu Ye,Bingqian Guo,Deyun Liu,Haibiao Yan,Tianyu Li,Qingyun Zhang,Jiwen Cheng,Zengnan Mo

Pediatric research 84:813-820 PubMed30341414

2018

Impaired antimicrobial response and mucosal protection induced by ibuprofen in the immature human intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuela Ferretti,Eric Tremblay,Marie-Pierre Thibault,Sepideh Fallah,David Grynspan,Karolina M Burghardt,Marcos Bettolli,Corentin Babakissa,Emile Levy,Jean-François Beaulieu

Acta histochemica et cytochemica 51:9-20 PubMed29622846

2018

Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets.

Applications

Unspecified application

Species

Unspecified reactive species

Sei Saitoh,Nobuhiko Ohno,Yurika Saitoh,Nobuo Terada,Satoshi Shimo,Kaoru Aida,Hideki Fujii,Tetsuro Kobayashi,Shinichi Ohno

Clinical cancer research : an official journal of the American Association for Cancer Research 21:3512-21 PubMed26240291

2015

Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz P Radon,Nathalie J Massat,Richard Jones,Wasfi Alrawashdeh,Laurent Dumartin,Darren Ennis,Stephen W Duffy,Hemant M Kocher,Stephen P Pereira,Luisa Guarner posthumous,Cristiane Murta-Nascimento,Francisco X Real,Núria Malats,John Neoptolemos,Eithne Costello,William Greenhalf,Nick R Lemoine,Tatjana Crnogorac-Jurcevic
View all publications
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