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AB251357

Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free

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Rabbit Recombinant Monoclonal REG3A antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.

View Alternative Names

HIP, PAP, PAP1, REG3A, Regenerating islet-derived protein 3-alpha, REG-3-alpha, Hepatointestinal pancreatic protein, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, HIP/PAP, Reg III-alpha

5 Images
Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)
  • WB

Supplier Data

Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)

This data was developed using ab202057, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literatures PMID : 12890698 & 22943287 for REG3A and PMID : 22252863 & 21254165 for REG3G.

Please note both Human fetal liver and brain represent negative controls for REG3A and REG3G.

All lanes:

Western blot - Anti-REG3A + REG3G antibody [EPRR18188] (<a href='/en-us/products/primary-antibodies/reg3a-reg3g-antibody-eprr18188-ab202057'>ab202057</a>) at 1/1000 dilution

Lane 1:

Human small intestine tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa,35 kDa,66 kDa

Observed band size: 16 kDa,39 kDa,67 kDa

false

Exposure time: 3min

Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)

This data was developed using ab202057, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling REG3G + REG3A with ab202057 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202057 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)

This data was developed using ab202057, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling REG3G + REG3A with ab202057 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202057 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)

This data was developed using ab202057, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling REG3G + REG3A with ab202057 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human pancreas tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)
  • WB

Supplier Data

Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (AB251357)

This data was developed using ab202057, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-REG3A + REG3G antibody [EPRR18188] (<a href='/en-us/products/primary-antibodies/reg3a-reg3g-antibody-eprr18188-ab202057'>ab202057</a>) at 1/10000 dilution

Lane 1:

REG3A recombinant protein fragment at 0.01 µg

Lane 2:

REG3G recombinant protein fragment at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 16 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPRR18188

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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AB267626

Human REG3A ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab251357 is the carrier-free version of ab202057.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REG3A also known as regenerating islet-derived protein 3 alpha and REG3G also known as regenerating islet-derived protein 3 gamma are small secretory proteins with molecular weights around 19 kDa. They are expressed mostly in the pancreas and gut epithelium. REG3A and REG3G contribute to the immune defense of the gastrointestinal tract. Their expression increases in response to inflammation and microbial presence acting as antimicrobial proteins that contribute to maintaining gut microbiota balance.
Biological function summary

Regenerating islet-derived proteins promote epithelial cell survival and are important in the mucosal immune response. Though not part of a larger complex these proteins bind to bacterial cell walls and disrupt their integrity causing lysis. They also recruit immune cells to sites of injury assisting in tissue repair. This ability highlights their role in coordinating the innate immune response within the gut.

Pathways

REG3A and REG3G participate in the intestinal innate immune pathways where they enhance host defense mechanisms against bacterial infections. They are interrelated with the Toll-like receptor signaling pathway which helps detect microbial patterns and initiate immune responses. REG3 proteins work in conjunction with other antimicrobial peptides to amplify the intestinal immune defense and contribute to regulation of inflammatory responses.

Both proteins associate with inflammatory bowel diseases like Crohn's disease and ulcerative colitis. Increased levels of REG3A and REG3G occur in patients with these disorders indicating their possible involvement in mucosal inflammation and host-pathogen interactions. Their expression links to cytokines such as IL-22 and IL-17 which are upregulated during inflammatory responses suggesting that REG3 proteins might act in modulating pathways that sustain chronic inflammation in gut-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regenerating islet-derived protein 3-alpha 15 kDa form. Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan (PubMed : 16931762). Binds membrane phospholipids and kills bacteria by forming a hexameric membrane-permeabilizing oligomeric pore (PubMed : 24256734).. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways (PubMed : 19158046, PubMed : 22727489, PubMed : 27830702, PubMed : 34099862). Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway (PubMed : 22727489). In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF (PubMed : 27830702). In pancreas, is able to permealize beta-cells membrane and stimulate their proliferation (PubMed : 19158046).. Regenerating islet-derived protein 3-alpha 16.5 kDa form. Has bacteriostatic activity.
See full target information REG3A

Additional targets

REG3G
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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