Rabbit Polyclonal REG3G antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human REG3G aa 1 to C-terminus.
Images
Publications
Filter by
- Frontiers in nutrition 8:6605982021Bovine Lactoferrin Protects Dextran Sulfate Sodium Salt Mice Against Inflammation and Impairment of Colonic Epithelial Barrier by Regulating Gut Microbial Structure and Metabolites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShalong Wang,Jingyu Zhou,Da Xiao,Guoshun Shu,Li GuPubMed 33954162
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human REG3G aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UW15
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Is secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways. Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury. In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF. Induced by IL22 in lung epithelial cells, inhibits cytokine production and regulates allergic airway inflammation. Induced in small intestine by inulin-enriched diet and Lactobacillus gasseri enriched microbiome, plays a role in the improvement of gut barrier function, the regulation of energy balance and glucose levels. Modulates microbiota composition in duodenal contents. Produced by nociceptor in response to endotoxins, prevents endotoxic death by targeting kynurenine pathway in microglia. Regenerating islet-derived protein 3-gamma 16.5 kDa form. Has bacteriostatic activity. Regenerating islet-derived protein 3-gamma 15 kDa form. Has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus.
Alternative names
PAP1B, UNQ429/PRO162, REG3G, Regenerating islet-derived protein 3-gamma, REG-3-gamma, Pancreatitis-associated protein 1B, Pancreatitis-associated protein IB, Regenerating islet-derived protein III-gamma, PAP-1B, PAP IB, REG III, Reg III-gamma
Recommended products
Rabbit Polyclonal REG3G antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human REG3G aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human REG3G aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UW15
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab233480 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
REG3G also known as Regenerating islet-derived protein 3-gamma is a member of the C-type lectin family. It has a molecular weight of about 19 kDa. This protein is expressed mainly in the epithelial cells of the intestine particularly in the ileum and colon. REG3G also shows expression in the pancreas and liver. The primary function of REG3G involves its bactericidal activity where it binds to peptidoglycan in the bacterial cell wall and disrupts it to kill bacteria.
- Biological function summary
REG3G plays a role in innate immune responses by maintaining homeostasis in the intestinal mucosa. It is not reported as part of a larger protein complex. REG3G helps modulate the gut microbiota and provides protection against pathogenic bacteria. The ability to control bacterial populations in the gut and prevent invasion highlights the protein’s role in the body's defense mechanisms.
- Pathways
REG3G participates in the antimicrobial peptide pathway contributing to mucosal immunity. This pathway plays an important role in the gastrointestinal tract where it prevents infection by pathogens. REG3G is related to other antimicrobial peptides such as REG3A and defensins which collectively enhance the mucosal barrier and protect against microbial invasion.
- Associated diseases and disorders
REG3G is linked to inflammatory bowel disease (IBD) and its expression levels can indicate the inflammatory state of the intestine. Studies have shown that changes in REG3G levels relate to Crohn's disease. Moreover REG3G has connections to diabetes particularly in terms of pancreatic beta-cell stress. It shares functional roles with proteins like REG3B suggesting a broader involvement in tissue regeneration processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab233480)
Paraffin-embedded human pancreatic cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab233480)
Paraffin-embedded human gallbladder cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab233480)
Paraffin-embedded human stomach cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab233480)
Paraffin-embedded human stomach tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab233480)
Paraffin-embedded human prostate cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-REG3G antibody (ab233480)
All lanes: Western blot - Anti-REG3G antibody (ab233480) at 3 µg/mL
All lanes: Pig cerebrum lysate
Predicted band size: 19 kDa
Western blot - Anti-REG3G antibody (ab233480)
All lanes: Western blot - Anti-REG3G antibody (ab233480) at 3 µg/mL
All lanes: Pig small intestine lysate
Predicted band size: 19 kDa
Western blot - Anti-REG3G antibody (ab233480)
All lanes: Western blot - Anti-REG3G antibody (ab233480) at 2 µg/mL
All lanes: Recombinant human REG3G protein
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab233480)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com