AB233480

Anti-REG3G antibody

Name: Anti-REG3G antibody (ab233480) | Abcam
Brand: Abcam Limited
SKU: AB233480
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal REG3G antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human REG3G aa 1 to C-terminus.

View Alternative Names

PAP1B, UNQ429/PRO162, REG3G, Regenerating islet-derived protein 3-gamma, REG-3-gamma, Pancreatitis-associated protein 1B, Pancreatitis-associated protein IB, Regenerating islet-derived protein III-gamma, PAP-1B, PAP IB, REG III, Reg III-gamma

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Paraffin-embedded human stomach tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Paraffin-embedded human pancreatic cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Paraffin-embedded human gallbladder cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Paraffin-embedded human prostate cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Paraffin-embedded human stomach cancer tissue stained for REG3G using ab233480 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-REG3G antibody (AB233480)

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Western blot - Anti-REG3G antibody (ab233480) at 3 µg/mL

All lanes:

Pig cerebrum lysate

Predicted band size: 19 kDa

false

Supplier Data

Western blot - Anti-REG3G antibody (AB233480)

All lanes:

Western blot - Anti-REG3G antibody (ab233480) at 3 µg/mL

All lanes:

Pig small intestine lysate

Predicted band size: 19 kDa

false

Supplier Data

Western blot - Anti-REG3G antibody (AB233480)

All lanes:

Western blot - Anti-REG3G antibody (ab233480) at 2 µg/mL

All lanes:

Recombinant human REG3G protein

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human REG3G aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6UW15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab233480 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REG3G also known as Regenerating islet-derived protein 3-gamma is a member of the C-type lectin family. It has a molecular weight of about 19 kDa. This protein is expressed mainly in the epithelial cells of the intestine particularly in the ileum and colon. REG3G also shows expression in the pancreas and liver. The primary function of REG3G involves its bactericidal activity where it binds to peptidoglycan in the bacterial cell wall and disrupts it to kill bacteria.

Biological function summary

REG3G plays a role in innate immune responses by maintaining homeostasis in the intestinal mucosa. It is not reported as part of a larger protein complex. REG3G helps modulate the gut microbiota and provides protection against pathogenic bacteria. The ability to control bacterial populations in the gut and prevent invasion highlights the protein’s role in the body's defense mechanisms.

Pathways

REG3G participates in the antimicrobial peptide pathway contributing to mucosal immunity. This pathway plays an important role in the gastrointestinal tract where it prevents infection by pathogens. REG3G is related to other antimicrobial peptides such as REG3A and defensins which collectively enhance the mucosal barrier and protect against microbial invasion.

REG3G is linked to inflammatory bowel disease (IBD) and its expression levels can indicate the inflammatory state of the intestine. Studies have shown that changes in REG3G levels relate to Crohn's disease. Moreover REG3G has connections to diabetes particularly in terms of pancreatic beta-cell stress. It shares functional roles with proteins like REG3B suggesting a broader involvement in tissue regeneration processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota.. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Is secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways. Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury. In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF. Induced by IL22 in lung epithelial cells, inhibits cytokine production and regulates allergic airway inflammation. Induced in small intestine by inulin-enriched diet and Lactobacillus gasseri enriched microbiome, plays a role in the improvement of gut barrier function, the regulation of energy balance and glucose levels. Modulates microbiota composition in duodenal contents. Produced by nociceptor in response to endotoxins, prevents endotoxic death by targeting kynurenine pathway in microglia.. Regenerating islet-derived protein 3-gamma 16.5 kDa form. Has bacteriostatic activity.. Regenerating islet-derived protein 3-gamma 15 kDa form. Has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus.

See full target information REG3G

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:24 PubMed38195677

2024

TNFα modulates PANX1 activation to promote ATP release and enhance P2RX7-mediated antitumor immune responses after chemotherapy in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Chih-Yang Huang,Shu-Fen Chiang,Pei-Chun Lin,Wei-Ze Hong,Pei-Chen Yang,Hui-Ping Chang,Shin-Lei Peng,Tsung-Wei Chen,Tao-Wei Ke,Ji-An Liang,William Tzu-Liang Chen,K S Clifford Chao

Cell host & microbe 31:1450-1468.e8 PubMed37652008

2023

Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Ku Jang,Thomas Heaney,Mariya London,Yi Ding,Gregory Putzel,Frank Yeung,Defne Ercelen,Ying-Han Chen,Jordan Axelrad,Sakteesh Gurunathan,Chaoting Zhou,Magdalena Podkowik,Natalia Arguelles,Anusha Srivastava,Bo Shopsin,Victor J Torres,A Marijke Keestra-Gounder,Alejandro Pironti,Matthew E Griffin,Howard C Hang,Ken Cadwell

Frontiers in nutrition 8:660598 PubMed33954162

2021

Bovine Lactoferrin Protects Dextran Sulfate Sodium Salt Mice Against Inflammation and Impairment of Colonic Epithelial Barrier by Regulating Gut Microbial Structure and Metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Shalong Wang,Jingyu Zhou,Da Xiao,Guoshun Shu,Li Gu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-REG3G antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (AB233480)

Western blot - Anti-REG3G antibody (AB233480)

Western blot - Anti-REG3G antibody (AB233480)

Western blot - Anti-REG3G antibody (AB233480)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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