Rabbit Recombinant Monoclonal REG4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (AB280998), expandable thumbnail](https://content.abcam.com/products/images/reg4-antibody-epr22810-281-bsa-and-azide-free-detector-ab280998--sandwich-elisa-img429532.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (AB280998)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
Alternative names
GISP, RELP, REG4, Regenerating islet-derived protein 4, REG-4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, Reg IV
Recommended products
Rabbit Recombinant Monoclonal REG4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22810-281
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab280998 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human REG4. The recommended pair for sandwich ELISA is:
Capture: Anti-REG4 antibody [EPR22810-49] - BSA and Azide free (Capture) ab281148, Human REG4 Capture Antibody (unconjugated)
Detector: ab280998, Human REG4 Detector Antibody (unconjugated)
The reference range value is 15.63 - 2000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
REG4 also known as Reg IV or regenerating islet-derived 4 is a protein with a mass of about 19 kDa. It belongs to the regenerating gene family and has roles in cell growth and regeneration. REG4 expression occurs mainly in the gastric antrum small intestine colon and pancreas. It also appears in certain epithelial tissues particularly ones undergoing regeneration or transformation. REG4's structure includes a C-type lectin domain which suggests its potential involvement in cell adhesion or signaling.
- Biological function summary
REG4 strengthens the protection of epithelial cells and supports regeneration during tissue damage or inflammation. It acts as part of cell growth and survival pathways and does not form large complexes on its own. The protein can promote proliferation and maintain integrity in epithelial tissues influencing both normal and cancerous cell behavior. The presence of REG4 might modulate cell response to environmental stress and impact cellular differentiation processes.
- Pathways
REG4 impacts both the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell proliferation and survival indicating REG4's involvement in cellular stress response and oncogenesis. REG4 interacts with other proteins such as EGFR (Epidermal Growth Factor Receptor) which participate in these pathways. This protein co-activity may enhance cellular responses contributing to growth regulation and survival signaling in various tissues.
- Associated diseases and disorders
REG4 has a strong association with colorectal cancer and inflammatory bowel disease (IBD). Its overexpression has been noted in colorectal cancer tissues and can relate to tumor growth and metastasis. In the context of IBD REG4 expression connects with inflammation markers and mucosal healing processes. Proteins like MUC2 (Mucin 2) and CDX2 (Caudal Type Homeobox 2) involved in these diseases show relationships with REG4 emphasizing its role in disease progression and regulation within epithelial tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (ab280998), expandable thumbnail](https://content.abcam.com/products/images/reg4-antibody-epr22810-281-bsa-and-azide-free-detector-ab280998--sandwich-elisa-img429532.jpg "Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (ab280998)")
Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (ab280998)
Sandwich ELISA of Human REG4 Antibody Pair - BSA and Azide free ab253588 with the capture antibody Anti-REG4 antibody [EPR22810-49] - BSA and Azide free (Capture) ab281148 dilution at 2 µg/mL and detector antibody ab280998 dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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