Rabbit Polyclonal Rel B antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RELB aa 350-550.
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- Life science alliance 5:2022Lack of bombesin receptor-activated protein attenuates bleomycin-induced pulmonary fibrosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Wang,Wenrui Zhang,Rujiao Liu,Jiaoyun Zheng,Xueping Yao,Hui Chen,Jie Wang,Horst Christian Weber,Xiaoqun Qin,Yang Xiang,Chi Liu,Huijun Liu,Lang Pan,Xiangping QuPubMed 35820707
- OncoTargets and therapy 13:1037-10462020miR-873-5p Inhibits Cell Migration and Invasion of Papillary Thyroid Cancer via Regulation of CXCL16.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenglin Wang et. al.PubMed 32099406
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 109:1287-12952018I-κB kinase-ε knockout protects against angiotensin II induced aortic valve thickening in apolipoprotein E deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuai He,Fulai Nian,Wen Chen,Li Yin,Merveesh L Auchoybur,Zhonghao Tao,Shaowen Tang,Xin ChenPubMed 30463808
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RELB aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q01201
Reactivity data
Select an application| IP | ChIP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function (PubMed:26385063).
Alternative names
Transcription factor RelB, I-Rel, RELB
Recommended products
Rabbit Polyclonal Rel B antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RELB aa 350-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RELB aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q01201
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rel B also known as RELB proto-oncogene is a member of the NF-κB family of transcription factors. This protein plays a significant role in regulating gene expression. Rel B has a molecular mass of approximately 68 kDa. It expresses in diverse tissues including lymphoid organs and epithelial cells. It generally resides in the cytoplasm and translocates to the nucleus upon activation.
- Biological function summary
Rel B serves as a critical component of the alternative NF-κB signaling pathway. It often forms a complex with another NF-κB family member called p52. This complex controls the transcription of genes involved in the immune response cell survival and differentiation. Additionally Rel B contributes to the development of secondary lymphoid organs and modulation of immune responses.
- Pathways
Rel B integrates into the non-canonical NF-κB signaling pathway also influencing the canonical pathway to a lesser extent. In the non-canonical pathway Rel B interacts with NIK and p100 to influence immune cell function. This involvement makes Rel B essential in processes like adaptive immunity and inflammatory responses. The protein p52 closely associates with Rel B aiding its activity in these pathways.
- Associated diseases and disorders
Rel B has connections to autoimmune diseases and certain cancers. Abnormal Rel B activity can lead to chronic inflammation and contribute to conditions like rheumatoid arthritis. In cancer its deregulation associates with lymphomas where it may interact with other NF-κB proteins like p50 and RelA affecting cell proliferation and survival. Understanding Rel B in these contexts could provide insights into therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
ChIP - Anti-Rel B antibody (ab154957)
Cross-linked ChIP was performed with THP-1 (human monocytic leukemia cell line) chromatin extract treated with LPS (1.0 μg/ml for 3 h) and 5 μg of either normal rabbit IgG or ab154957. The precipitated DNA was detected by PCR with primer set targeting to IL-1β promoter.
Immunoprecipitation - Anti-Rel B antibody (ab154957)
RelB antibody immunoprecipitates RelB protein in IP experiments.
IP Sample: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of RelB protein by 2 μg of ab154957
7.5% SDS-PAGE
The immunoprecipitated RelB protein was detected by ab154957 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
All lanes: Immunoprecipitation - Anti-Rel B antibody (ab154957)
Predicted band size: 62 kDa
Western blot - Anti-Rel B antibody (ab154957)
All lanes: Western blot - Anti-Rel B antibody (ab154957) at 1/10000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Lane 3: NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rel B antibody (ab154957)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue staining Rel B at cytoplasm and nucleus with ab154957 at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-Rel B antibody (ab154957)
7.5% SDS-PAGE
All lanes: Western blot - Anti-Rel B antibody (ab154957) at 1/5000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: Transfected HEK-293T whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa
Western blot - Anti-Rel B antibody (ab154957)
7.5% SDS PAGE
All lanes: Western blot - Anti-Rel B antibody (ab154957) at 1/10000 dilution
All lanes: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa
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