AB183505

Anti-Relaxin 2/RLN2 antibody [EPR14205]

Name: Anti-Relaxin 2/RLN2 antibody [EPR14205] (ab183505) | Abcam
Brand: Abcam Limited
SKU: AB183505
Price: 520 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal Relaxin 2/RLN2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Prorelaxin H2, RLN2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Relaxin 2/RLN2 antibody [EPR14205] (AB183505)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Relaxin 2/RLN2 antibody [EPR14205] (AB183505)

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling pro Relaxin 2/RLN2 with ab183505 at 1/500 followed by prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Relaxin 2/RLN2 antibody [EPR14205] (AB183505)

All lanes:

Western blot - Anti-Relaxin 2/RLN2 antibody [EPR14205] (ab183505) at 1/5000 dilution

All lanes:

K562 cell line lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 21 kDa

false

Carrier free

Anti-Relaxin 2/RLN2 antibody [EPR14205] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14205

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Relaxin 2 (RLN2) also known as human relaxin is a peptide hormone with a molecular mass of approximately 6 kDa. It belongs to the insulin superfamily. This hormone is secreted primarily by the corpus luteum during pregnancy but also gets expression in other tissues like the heart and kidney. Relaxin 2 plays an important role in reproductive physiology facilitating a range of mechanical processes essential for pregnancy such as remodeling the extracellular matrix and influencing the dilation of the cervix.

Biological function summary

The role of human relaxin involves modulating tissue growth and remodeling. It works by interacting with relaxin family peptide receptors mainly RXFP1. It is not part of a larger complex but has autonomous structural and functional capabilities. The hormone influences collagen metabolism and fibroblast activity enhancing the extensibility and elasticity of the tissues where it is active.

Pathways

Researchers have identified relaxin 2 as a critical component in the relaxin signaling pathway and involved in the transforming growth factor-beta (TGF-beta) pathway. Its interaction with RXFP1 activates several intracellular signaling cascades including adenylate cyclase/cAMP pathway and phosphatidylinositol 3-kinase pathway affecting proteins like collagen and matrix metalloproteinases. These pathways play a fundamental role in connective tissue remodeling and have implications in normal and pathological states.

Studies connect relaxin 2 to conditions such as cardiac fibrosis and scleroderma. In cardiac fibrosis relaxin 2 influences matrix metalloproteinases and collagen metabolism aiming to reduce pathological tissue stiffness. In scleroderma relaxin 2's potential to modulate excessive collagen deposition links it to mitigating disease symptoms alongside proteins like TGF-beta which play a part in the disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

See full target information RLN2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biochemical pharmacology 225:116323 PubMed38815632

2024

Relaxin-2 is a novel biomarker for differentiated thyroid carcinoma in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Anupam Kotwal,Ronda Simpson,Nicholas Whiteman,Benjamin Swanson,Ana Yuil-Valdes,Madelyn Fitch,Joshua Nguyen,Salma Elhag,Oleg Shats,Whitney Goldner,Robert Bennett

The Journal of clinical investigation 131: PubMed33561012

2021

Inhibition of relaxin autocrine signaling confers therapeutic vulnerability in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Helen E Burston,Oliver A Kent,Laudine Communal,Molly L Udaskin,Ren X Sun,Kevin R Brown,Euihye Jung,Kyle E Francis,Jose La Rose,Joshua Lowitz,Ronny Drapkin,Anne-Marie Mes-Masson,Robert Rottapel

International journal of molecular sciences 19: PubMed30126180

2018

Relaxin 2/RXFP1 Signaling Induces Cell Invasion via the β-Catenin Pathway in Endometrial Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Misaki Fue,Yasuhiro Miki,Kiyoshi Takagi,Chiaki Hashimoto,Nobuo Yaegashi,Takashi Suzuki,Kiyoshi Ito

F1000Research 7:1189 PubMed30271587

2018

Androgen-dependent alternative mRNA isoform expression in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Munkley,Teresa M Maia,Nekane Ibarluzea,Karen E Livermore,Daniel Vodak,Ingrid Ehrmann,Katherine James,Prabhakar Rajan,Nuno L Barbosa-Morais,David J Elliott

FASEB journal : official publication of the Federa :fj201800437R PubMed29882709

2018

Relaxin receptor deficiency promotes vascular inflammation and impairs outward remodeling in arteriovenous fistulas.

Applications

Unspecified application

Species

Unspecified reactive species

Taisiya Bezhaeva,Margreet R de Vries,Wouter J Geelhoed,Eric P van der Veer,Sabine Versteeg,Carla M A van Alem,Bram M Voorzaat,Niels Eijkelkamp,Koen E van der Bogt,Alexander I Agoulnik,Anton-Jan van Zonneveld,Paul H A Quax,Joris I Rotmans

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Relaxin 2/RLN2 antibody [EPR14205]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Relaxin 2/RLN2 antibody [EPR14205] (AB183505)

Western blot - Anti-Relaxin 2/RLN2 antibody [EPR14205] (AB183505)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise