JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259533

Anti-Relaxin 2/RLN2 antibody [EPR21832-15] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Relaxin 2/RLN2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Prorelaxin H2, RLN2

1 Images
Sandwich ELISA - Anti-Relaxin 2/RLN2 antibody [EPR21832-15] - BSA and Azide free (Capture) (AB259533)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Relaxin 2/RLN2 antibody [EPR21832-15] - BSA and Azide free (Capture) (AB259533)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab243688).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21832-15

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab259533 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Relaxin-2. The recommended pair for sandwich ELISA is:
Capture: ab259533, Human Relaxin-2 Capture Antibody (unconjugated)
Detector: ab259534, Human Relaxin-2 Detector Antibody (unconjugated)
The reference range value is 2.03 - 130 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Relaxin 2 (RLN2) also known as human relaxin is a peptide hormone with a molecular mass of approximately 6 kDa. It belongs to the insulin superfamily. This hormone is secreted primarily by the corpus luteum during pregnancy but also gets expression in other tissues like the heart and kidney. Relaxin 2 plays an important role in reproductive physiology facilitating a range of mechanical processes essential for pregnancy such as remodeling the extracellular matrix and influencing the dilation of the cervix.
Biological function summary

The role of human relaxin involves modulating tissue growth and remodeling. It works by interacting with relaxin family peptide receptors mainly RXFP1. It is not part of a larger complex but has autonomous structural and functional capabilities. The hormone influences collagen metabolism and fibroblast activity enhancing the extensibility and elasticity of the tissues where it is active.

Pathways

Researchers have identified relaxin 2 as a critical component in the relaxin signaling pathway and involved in the transforming growth factor-beta (TGF-beta) pathway. Its interaction with RXFP1 activates several intracellular signaling cascades including adenylate cyclase/cAMP pathway and phosphatidylinositol 3-kinase pathway affecting proteins like collagen and matrix metalloproteinases. These pathways play a fundamental role in connective tissue remodeling and have implications in normal and pathological states.

Studies connect relaxin 2 to conditions such as cardiac fibrosis and scleroderma. In cardiac fibrosis relaxin 2 influences matrix metalloproteinases and collagen metabolism aiming to reduce pathological tissue stiffness. In scleroderma relaxin 2's potential to modulate excessive collagen deposition links it to mitigating disease symptoms alongside proteins like TGF-beta which play a part in the disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.
See full target information RLN2
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB243688

Human Relaxin-2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB243688

Human Relaxin-2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

  • 0
  • 0
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com