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Rabbit Recombinant Monoclonal LUCI antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Renilla reniformis, Transfected cell line - Renilla reniformis samples. Cited in 7 publications.


Images

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (AB185925), expandable thumbnail
  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925), expandable thumbnail
  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925), expandable thumbnail
  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Renilla reniformis
Not recommended
Not recommended
Transfected cell line - Renilla reniformis
Not recommended
Tested
Transfected cell lysate - Renilla reniformis
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Renilla reniformis
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Renilla reniformis, Transfected cell line - Renilla reniformis
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Renilla reniformis
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Renilla reniformis, Transfected cell lysate - Renilla reniformis
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

Alternative names

Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

Recommended products

Rabbit Recombinant Monoclonal LUCI antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Renilla reniformis, Transfected cell line - Renilla reniformis samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17791
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Renilla Luciferase also known as RLuc is an enzyme that catalyzes the oxidation of its substrate coelenterazine producing bioluminescence. With a molecular mass of approximately 36 kDa RLuc facilitates this reaction by oxygenation resulting in the emission of blue light. Renilla Luciferase is typically isolated from the sea pansy Renilla reniformis and has been widely used as a reporter gene in molecular biology for monitoring gene expression and cellular events due to its efficient light production.

Biological function summary

Renilla Luciferase operates as part of the bioluminescent system found in certain marine organisms. In its native environment it interacts with other proteins like Green Fluorescent Protein (GFP) to which it transfers energy converting blue light to green fluorescence. This process of bioluminescence serves various ecological functions including predator avoidance and communication between organisms. These interactions highlight the importance of Renilla Luciferase in understanding biological luminescence systems.

Pathways

Researchers use Renilla Luciferase extensively in signaling and metabolic pathway analyses. It plays an important role in the study of G-protein coupled receptor (GPCR) pathways where it can help to analyze receptor activation and downstream communication. In these pathways it often pairs with other luminescent or fluorescent markers like Firefly Luciferase or GFP enabling simultaneous dual-reporter assays to provide more comprehensive data on cellular responses.

Associated diseases and disorders

Renilla Luciferase aids in the study of cancer and infectious diseases. Its role in reporter assays facilitates the investigation of gene expression changes and the screening of potential therapeutic compounds. For example in cancer research it is frequently paired with p53 proteins for studying apoptosis and cell cycle regulation. Similarly in infectious disease studies Renilla Luciferase reporters can track viral infections and the efficacy of antiviral drugs. These applications highlight its value in advancing biomedical research and drug development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925), expandable thumbnail

    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

    All lanes: Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1: Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

    Lane 2: Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (ab185925), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185925 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab185925 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925), expandable thumbnail

    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1: Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

    Lane 2: Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) lysate at 20 µg

    Lane 5: Jurkat (Human T cell leukemia cells from peripheral blood) lysate at 20 µg

    Lane 6: SH-SY5Y (Human neuroblastoma from bone marrow cells) lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

    Exposure time: 3min

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925), expandable thumbnail

    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1: Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

    Lane 2: Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: Human fetal brain lysate at 10 µg

    Lane 4: Human fetal heart lysate at 10 µg

    Lane 5: Human fetal kidney lysate at 10 µg

    Lane 6: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

    Exposure time: 3min

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925), expandable thumbnail

    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1: Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

    Lane 2: Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

    Lane 3: Mouse brain lysate at 10 µg

    Lane 4: Mouse heart lysate at 10 µg

    Lane 5: Mouse kidney lysate at 10 µg

    Lane 6: Mouse spleen lysate at 10 µg

    Lane 7: Rat brain lysate at 10 µg

    Lane 8: Rat heart lysate at 10 µg

    Lane 9: Rat kidney lysate at 10 µg

    Lane 10: Rat spleen lysate at 10 µg

    Lane 11: C6 (Rat glial tumor cells) lysate at 10 µg

    Lane 12: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

    Lane 13: PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

    Lane 14: NIH/3T3 (Mouse embyro fibroblast cells) lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa, 43 kDa

    Observed band size: 36 kDa

    Exposure time: 3min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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