AB185925

Anti-Renilla Luciferase antibody [EPR17791]

Name: Anti-Renilla Luciferase antibody [EPR17791] (ab185925) | Abcam
Brand: Abcam Limited
SKU: AB185925
Price: 550 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal LUCI antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Renilla reniformis, Transfected cell line - Renilla reniformis samples. Cited in 9 publications.

View Alternative Names

Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185925 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1 : ab185925 at 1/1000 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Supplier Data

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

All lanes:

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1:

Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

Lane 2:

Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1:

Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1:

Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

Lane 2:

Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 3:

Mouse brain lysate at 10 µg

Lane 4:

Mouse heart lysate at 10 µg

Lane 5:

Mouse kidney lysate at 10 µg

Lane 6:

Mouse spleen lysate at 10 µg

Lane 7:

Rat brain lysate at 10 µg

Lane 8:

Rat heart lysate at 10 µg

Lane 9:

Rat kidney lysate at 10 µg

Lane 10:

Rat spleen lysate at 10 µg

Lane 11:

C6 (Rat glial tumor cells) lysate at 10 µg

Lane 12:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

Lane 13:

PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

Lane 14:

NIH/3T3 (Mouse embyro fibroblast cells) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa,43 kDa

Observed band size: 36 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

Lane 1:

Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) lysate at 20 µg

Lane 5:

Jurkat (Human T cell leukemia cells from peripheral blood) lysate at 20 µg

Lane 6:

SH-SY5Y (Human neuroblastoma from bone marrow cells) lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Exposure time: 3min

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Renilla Luciferase antibody [EPR17791]
Carrier free

Anti-Renilla Luciferase antibody [EPR17791] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17791

Isotype

IgG

Carrier free

Reacts with

Renilla reniformis

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Renilla reniformis": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Renilla reniformis": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Transfected cell lysate - Renilla reniformis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renilla Luciferase also known as RLuc is an enzyme that catalyzes the oxidation of its substrate coelenterazine producing bioluminescence. With a molecular mass of approximately 36 kDa RLuc facilitates this reaction by oxygenation resulting in the emission of blue light. Renilla Luciferase is typically isolated from the sea pansy Renilla reniformis and has been widely used as a reporter gene in molecular biology for monitoring gene expression and cellular events due to its efficient light production.

Biological function summary

Renilla Luciferase operates as part of the bioluminescent system found in certain marine organisms. In its native environment it interacts with other proteins like Green Fluorescent Protein (GFP) to which it transfers energy converting blue light to green fluorescence. This process of bioluminescence serves various ecological functions including predator avoidance and communication between organisms. These interactions highlight the importance of Renilla Luciferase in understanding biological luminescence systems.

Pathways

Researchers use Renilla Luciferase extensively in signaling and metabolic pathway analyses. It plays an important role in the study of G-protein coupled receptor (GPCR) pathways where it can help to analyze receptor activation and downstream communication. In these pathways it often pairs with other luminescent or fluorescent markers like Firefly Luciferase or GFP enabling simultaneous dual-reporter assays to provide more comprehensive data on cellular responses.

Renilla Luciferase aids in the study of cancer and infectious diseases. Its role in reporter assays facilitates the investigation of gene expression changes and the screening of potential therapeutic compounds. For example in cancer research it is frequently paired with p53 proteins for studying apoptosis and cell cycle regulation. Similarly in infectious disease studies Renilla Luciferase reporters can track viral infections and the efficacy of antiviral drugs. These applications highlight its value in advancing biomedical research and drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

See full target information Coelenterazine h 2-monooxygenase

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 23:51 PubMed39985000

2025

Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Meng,Yuan Meng,Xuena Yang,Wenwen Yu,Bole Li,Tianjun Liu,Jie Zhang,Xiubao Ren,Lin Zhang

Nature genetics 56:2815-2826 PubMed39496881

2024

The ZmCPK39-ZmDi19-ZmPR10 immune module regulates quantitative resistance to multiple foliar diseases in maize.

Applications

Unspecified application

Species

Unspecified reactive species

Mang Zhu,Tao Zhong,Ling Xu,Chenyu Guo,Xiaohui Zhang,Yulin Liu,Yan Zhang,Yancong Li,Zhijian Xie,Tingting Liu,Fuyan Jiang,Xingming Fan,Peter Balint-Kurti,Mingliang Xu

PloS one 16:e0249928 PubMed33836032

2021

Mapping of sequences in the 5' region and 3' UTR of tomato ringspot virus RNA2 that facilitate cap-independent translation of reporter transcripts in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Dinesh Babu Paudel,Hélène Sanfaçon

Cell reports 33:108262 PubMed33053355

2020

4EHP and GIGYF1/2 Mediate Translation-Coupled Messenger RNA Decay.

Applications

Unspecified application

Species

Unspecified reactive species

Ramona Weber,Min-Yi Chung,Csilla Keskeny,Ulrike Zinnall,Markus Landthaler,Eugene Valkov,Elisa Izaurralde,Cátia Igreja

Antiviral research 173:104646 PubMed31705922

2019

Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Niu,Liang Shen,Baoying Huang,Fei Ye,Li Zhao,Huijuan Wang,Yao Deng,Wenjie Tan

Cellular and molecular life sciences : CMLS 77:2017-2027 PubMed31392347

2019

Translation of TNFAIP2 is tightly controlled by upstream open reading frames.

Applications

Unspecified application

Species

Unspecified reactive species

Anica Scholz,Peter Rappl,Nicola Böffinger,Ana Carolina Mota,Bernhard Brüne,Tobias Schmid

Human mutation 39:1360-1365 PubMed30040876

2018

Disruption of TWIST1 translation by 5' UTR variants in Saethre-Chotzen syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhou,Nils Koelling,Aimée L Fenwick,Simon J McGowan,Eduardo Calpena,Steven A Wall,Sarah F Smithson,Andrew O M Wilkie,Stephen R F Twigg

Cytotherapy 20:232-244 PubMed29167063

2017

Intramuscular administration potentiates extended dwell time of mesenchymal stromal cells compared to other routes.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena R Braid,Catherine A Wood,Danielle M Wiese,Barry N Ford

Antimicrobial agents and chemotherapy 60:5492-503 PubMed27381385

2016

Safe and Sensitive Antiviral Screening Platform Based on Recombinant Human Coronavirus OC43 Expressing the Luciferase Reporter Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Shen,Yang Yang,Fei Ye,Gaoshan Liu,Marc Desforges,Pierre J Talbot,Wenjie Tan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Renilla Luciferase antibody [EPR17791]

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

Western blot - Anti-Renilla Luciferase antibody [EPR17791] (AB185925)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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