AB185926

Anti-Renilla Luciferase antibody [EPR17792]

Name: Anti-Renilla Luciferase antibody [EPR17792] (ab185926) | Abcam
Brand: Abcam Limited
SKU: AB185926
Price: 340 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal LUCI antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Renilla reniformis samples. Cited in 6 publications.

View Alternative Names

Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185926 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab185926 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

Flow Cyt

Lab

Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Renilla Luciferase cells labeled with purified ab185926 at 1/70 dilution (10μg/ml, Right panel) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

Lab

Western blot - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

All lanes:

Western blot - Anti-Renilla Luciferase antibody [EPR17792] (ab185926) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with Renilla Luciferase expression vector, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

578 PE

PE Anti-Renilla Luciferase antibody [EPR17792]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792]
Carrier free

Anti-Renilla Luciferase antibody [EPR17792] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Renilla Luciferase antibody [EPR17792]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Renilla Luciferase antibody [EPR17792]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Renilla Luciferase antibody [EPR17792]
660 APC

APC Anti-Renilla Luciferase antibody [EPR17792]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17792

Isotype

IgG

Carrier free

Reacts with

Renilla reniformis

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Renilla reniformis": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renilla Luciferase also known as RLuc is an enzyme that catalyzes the oxidation of its substrate coelenterazine producing bioluminescence. With a molecular mass of approximately 36 kDa RLuc facilitates this reaction by oxygenation resulting in the emission of blue light. Renilla Luciferase is typically isolated from the sea pansy Renilla reniformis and has been widely used as a reporter gene in molecular biology for monitoring gene expression and cellular events due to its efficient light production.

Biological function summary

Renilla Luciferase operates as part of the bioluminescent system found in certain marine organisms. In its native environment it interacts with other proteins like Green Fluorescent Protein (GFP) to which it transfers energy converting blue light to green fluorescence. This process of bioluminescence serves various ecological functions including predator avoidance and communication between organisms. These interactions highlight the importance of Renilla Luciferase in understanding biological luminescence systems.

Pathways

Researchers use Renilla Luciferase extensively in signaling and metabolic pathway analyses. It plays an important role in the study of G-protein coupled receptor (GPCR) pathways where it can help to analyze receptor activation and downstream communication. In these pathways it often pairs with other luminescent or fluorescent markers like Firefly Luciferase or GFP enabling simultaneous dual-reporter assays to provide more comprehensive data on cellular responses.

Renilla Luciferase aids in the study of cancer and infectious diseases. Its role in reporter assays facilitates the investigation of gene expression changes and the screening of potential therapeutic compounds. For example in cancer research it is frequently paired with p53 proteins for studying apoptosis and cell cycle regulation. Similarly in infectious disease studies Renilla Luciferase reporters can track viral infections and the efficacy of antiviral drugs. These applications highlight its value in advancing biomedical research and drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

See full target information Coelenterazine h 2-monooxygenase

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111697 PubMed39898030

2025

Cdk6's functions are critically regulated by its unique C-terminus.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Schirripa,Helge Schöppe,Sofie Nebenfuehr,Markus Zojer,Thorsten Klampfl,Valentina Kugler,Belinda S Maw,Huriye Ceylan,Iris Z Uras,Lisa Scheiblecker,Elisabeth Gamper,Ulrich Stelzl,Eduard Stefan,Teresa Kaserer,Veronika Sexl,Karoline Kollmann

Nature communications 15:5609 PubMed38965228

2024

A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level.

Applications

Unspecified application

Species

Unspecified reactive species

Assunta Merolla,Caterina Michetti,Matteo Moschetta,Francesca Vacca,Lorenzo Ciano,Laura Emionite,Simonetta Astigiano,Alessandra Romei,Simone Horenkamp,Ken Berglund,Robert E Gross,Fabrizia Cesca,Elisabetta Colombo,Fabio Benfenati

Cell reports methods 4:100755 PubMed38608690

2024

A highly optimized human in vitro translation system.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Bothe,Nenad Ban

mBio 13:e0224322 PubMed35997284

2022

Marburg Virus VP30 Is Required for Transcription Initiation at the Glycoprotein Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Megan R Edwards,Olivia A Vogel,Hiroyuki Mori,Robert A Davey,Christopher F Basler

Journal of cardiovascular translational research 16:112-126 PubMed35900670

2022

Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Xinliang Zou,Yi Liao,Zhihui Liu,Xiang Xu,Weiwei Sun,Haoran Qin,Haidong Wang,Jianping Liu,Tao Jing

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 15:728-741 PubMed29736859

2018

Pigment Epithelium-Derived Factor Plays a Role in Alzheimer's Disease by Negatively Regulating Aβ42.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Huang,Weiwei Qi,Shuhuan Fang,Ping Jiang,Cong Yang,Yousheng Mo,Chang Dong,Yan Li,Jun Zhong,Weibin Cai,Zhonghan Yang,Ti Zhou,Qi Wang,Xia Yang,Guoquan Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Renilla Luciferase antibody [EPR17792]

Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

Flow Cytometry - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

Western blot - Anti-Renilla Luciferase antibody [EPR17792] (AB185926)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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