Anti-Renin-1+ Renin-2 antibody [EPR30219-25]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Renin with ab326049 at 1/2000 (0.26 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on juxtaglomerular cells in mouse kidney (PMID : 33508746).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining Renin with ab326049 at a 1 : 5000 (0.103 µg/ml) dilution ab317588 anti-CD34 used at 1 : 5000 (0.099 µg/ml) dilution and ab199975 anti-Aquaporin 2 used at a 1 : 4000 (0.152 µg/ml) dilution.

Panel A : merged staining of anti-Renin (green; Opal™520) anti-CD34 (magenta; Opal™690) and anti-Aquaporin 2 (gray; Opal™570) on mouse kidney.
Panel B : anti-Renin staining juxtaglomerular cells in mouse kidney.
Panel C : anti-CD34 staining endothelium in mouse kidney.
Panel D : anti-Aquaporin 2 staining collecting tubules in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326049 ab317588 and ab199975 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Renin with ab326049 at 1/2000 (0.26 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on juxtaglomerular cells in rat kidney.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue staining Renin with ab326049 at a 1 : 5000 (0.103 µg/ml) dilution ab317588 anti-CD34 used at 1 : 5000 (0.099 µg/ml) dilution and ab199975 anti-Aquaporin 2 used at a 1 : 4000 (0.152 µg/ml) dilution.

Panel A : merged staining of anti-Renin (green; Opal™520) anti-CD34 (magenta; Opal™690) and anti-Aquaporin 2 (gray; Opal™570) on rat kidney.
Panel B : anti-Renin staining juxtaglomerular cells in rat kidney.
Panel C : anti-CD34 staining endothelium in rat kidney.
Panel D : anti-Aquaporin 2 staining collecting tubules in rat kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326049 ab317588 and ab199975 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen.

The identity of the higher MW band at approximately 250 kDa (in lanes2, 3 and 6) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (ab326049) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 50 µg

Lane 2:

Mouse spleen tissue lysate at 50 µg

Lane 3:

Rat kidney tissue lysate at 50 µg

Lane 4:

Rat spleen tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa,50 kDa,36 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Renin with ab326049 at 1/2000 (0.26 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse lung.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Renin with ab326049 at 1/2000 (0.26 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Renin with ab326049 at 1/2000 (0.26 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Renin with ab326049 at 1/2000 (0.26 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on rat lung.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Dot

Lab

Dot Blot - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (AB326049)

Dot blot analysis of Renin using ab326049 at 1 : 1000 (0.519 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : His-tagged mouse Renin-1 recombinant protein

Lane 2 : His-tagged mouse Renin-2 recombinant protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody could react with mouse Renin-1 and Renin-2.

All lanes:

Dot Blot - Anti-Renin-1+ Renin-2 antibody [EPR30219-25] (ab326049) at 1/1000 dilution

Lane 1:

His-tagged mouse Renin-1 recombinant protein

Lane 2:

His-tagged mouse Renin-2 recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s