Rabbit Recombinant Monoclonal Renin antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130569.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197)")
![Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (AB212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-frozen-sections-img129886.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (AB212197)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28912.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197)")
![Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (AB212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-frozen-sections-img18343.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (AB212197)")
Publications
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- International journal of molecular sciences 23:2022Enhanced Cardiorenal Protective Effects of Combining SGLT2 Inhibition, Endothelin Receptor Antagonism and RAS Blockade in Type 2 Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnder Vergara,Conxita Jacobs-Cacha,Carmen Llorens-Cebria,Alberto Ortiz,Irene Martinez-Diaz,Nerea Martos,Pamela Dominguez-Báez,Mireia Molina Van den Bosch,Sheila Bermejo,Michael Paul Pieper,Begoña Benito,Maria Jose SolerPubMed 36361612
- Scientific reports 12:53892022A novel renal perivascular mesenchymal cell subset gives rise to fibroblasts distinct from classic myofibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShun Minatoguchi,Shoji Saito,Kazuhiro Furuhashi,Yuriko Sawa,Masaki Okazaki,Yuko Shimamura,Ahmad Baseer Kaihan,Yusaku Hashimoto,Yoshinari Yasuda,Akitoshi Hara,Yasuyuki Mizutani,Ryota Ando,Noritoshi Kato,Takuji Ishimoto,Naotake Tsuboi,Nobutoshi Esaki,Makoto Matsuyama,Yukihiro Shiraki,Hiroki Kobayashi,Naoya Asai,Atsushi Enomoto,Shoichi MaruyamaPubMed 35354870
- Scientific reports 12:41972022Piezo2 expression and its alteration by mechanical forces in mouse mesangial cells and renin-producing cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Mochida,Koji Ochiai,Takashi Nagase,Keiko Nonomura,Yoshihiro Akimoto,Hiroshi Fukuhara,Tatsuo Sakai,George Matsumura,Yoshihiro Yamaguchi,Miki NagasePubMed 35273307
- JCI insight 6:2021Inhibition of the renin-angiotensin system causes concentric hypertrophy of renal arterioles in mice and humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHirofumi Watanabe,Alexandre G Martini,Robin Isadora Brown,Xiuyin Liang,Silvia Medrano,Shin Goto,Ichiei Narita,Lois J Arend,Maria Luisa S Sequeira-Lopez,R Ariel GomezPubMed 34762601
- Cancers 13:2021The Extracellular Matrix Environment of Clear Cell Renal Cell Carcinoma Determines Cancer Associated Fibroblast Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyle H Bond,Takuto Chiba,Kieran P H Wynne,Calvin P H Vary,Sunder Sims-Lucas,Jeannine M Coburn,Leif OxburghPubMed 34884982
- STAR protocols 2:1007472021Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth K Neumann,Nathan Heath Patterson,Jamie L Allen,Lukasz G Migas,Haichun Yang,Maya Brewer,David M Anderson,Jennifer Harvey,Danielle B Gutierrez,Raymond C Harris,Mark P deCaestecker,Agnes B Fogo,Raf Van de Plas,Richard M Caprioli,Jeffrey M SpragginsPubMed 34430920
- Frontiers in nutrition 8:6394672021Network and 16S rRNA Sequencing-Combined Approach Provides Insightal Evidence of Vitamin K for Salt-Sensitive Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian-Hao Liu,Ming-Hao Chen,Wan-Qing Tu,Qiu-Er Liang,Wen-Cong Tao,Zhen Jin,Ya Xiao,Li-Guo ChenPubMed 33718421
- Kidney international 98:630-6442020Pannexin 1 channels in renin-expressing cells influence renin secretion and blood pressure homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeon J DeLalio et. al.PubMed 32446934
- Communications biology 3:2312020Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshwani Kumar Gupta et. al.PubMed 32393756
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Expected | Tested |
| Mouse | Not recommended | Tested | Tested |
| Rat | Not recommended | Tested | Expected |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes WB application is not recommended due to no signal in endogenous lysates. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes WB application is not recommended due to no signal in endogenous lysates. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes WB application is not recommended due to no signal in endogenous lysates. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/6000 | Notes - |
Species Rat | Dilution info 1/6000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes (for human); Use at 1/5000 dilution (for mouse). This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 | Notes (for human); Use at 1/5000 dilution (for mouse). This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Alternative names
Renin, Angiotensinogenase, REN
Recommended products
Rabbit Recombinant Monoclonal Renin antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20693
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Renin also known as angiotensinogenase is a proteolytic enzyme with a molecular weight of approximately 40 kDa. It is mainly expressed in the juxtaglomerular cells of the kidneys. Renin plays an important role in the renin-angiotensin system where it initiates the conversion of angiotensinogen to angiotensin I. This process is the first step in a cascade leading to a series of effects in blood pressure regulation and fluid balance.
- Biological function summary
The renin enzyme is responsible for blood pressure control and electrolyte homeostasis. It acts as a significant regulator within the renin-angiotensin aldosterone system (RAAS). By catalyzing the formation of angiotensin I from angiotensinogen renin sets off a sequence of reactions that influence vascular tone and sodium reabsorption. The activity of renin significantly interacts with various cardiovascular functions alongside complex regulatory feedback from other hormones such as aldosterone.
- Pathways
Renin is a pivotal part of RAAS and interlinks with multiple cardiovascular pathways. The RAAS pathway involves angiotensinogen renin angiotensin I converting enzyme (ACE) and angiotensin II. Angiotensin II then acts on its receptor to mediate vasoconstriction and aldosterone secretion. The direct relationship between renin and ACE places this enzyme in the center of blood pressure management and has implications on kidney function and homeostasis.
- Associated diseases and disorders
High renin activity has connections with hypertension and cardiovascular diseases. Elevated renin levels contribute to increased angiotensin II production causing vasoconstriction and blood pressure elevation. Disorders like renal hypertension see direct involvement of the renin-angiotensin system. Therapeutic approaches often target RAAS with angiotensin-converting enzyme inhibitors (ACE inhibitors) and angiotensin receptor blockers aiming to mitigate the renin-related impact on these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130569.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID: 1898348). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-frozen-sections-img129886.jpg "Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)")
Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID: 27080979).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28912.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID: 1898348). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197), expandable thumbnail](https://content.abcam.com/products/images/renin-antibody-epr20693-ab212197--immunohistochemistry-frozen-sections-img18343.jpg "Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)")
Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID: 27080979).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).
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