AB86057

Anti-RENT1/hUPF1 antibody

Name: Anti-RENT1/hUPF1 antibody (ab86057) | Abcam
Brand: Abcam Limited
SKU: AB86057

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(7 Publications)

Rabbit Polyclonal RENT1/hUPF1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 7 publications.

View Alternative Names

KIAA0221, RENT1, UPF1, Regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog, NORF1, hUpf1

3 Images

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Immunoprecipitation - Anti-RENT1/hUPF1 antibody (AB86057)

Detection of RENT1/hUPF1 by Western Blot of Immunprecipitate.
ab86057 at 1µg/ml staining RENT1/hUPF1 in HeLa whole cell lysate immunoprecipitated using ab86057 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-RENT1/hUPF1 antibody (ab86057)

Predicted band size: 124 kDa

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Western blot - Anti-RENT1/hUPF1 antibody (AB86057)

All lanes:

Western blot - Anti-RENT1/hUPF1 antibody (ab86057) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 124 kDa

Observed band size: 134 kDa

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Exposure time: 10s

CiteAb

Western blot - Anti-RENT1/hUPF1 antibody (AB86057)

RENT1/hUPF1 western blot using anti-RENT1/hUPF1 antibody ab86057. Publication image and figure legend from Trzaska, C., Amand, S., et al., 2020, Nat Commun, PubMed 32198346.

ab86057 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab86057 please see the product overview.

DAP interferes with the activity of FTSJ1.a Determination by tandem mass spectrometry of the amino acid incorporated at the PTC position. Firefly luciferase from HEK293FT cells transfected with the Fluc-int-UGA construct was immunoprecipitated and digested with trypsin. The MS/MS spectrum of the peptide containing the PTC position and the sequence determination are shown. The upper red and blue sequences indicate the amino acids validated, respectively, by detection of Nter and Cter peptide fragments (b and y ion series). Peptide fragments y6 and y7 clearly identify tryptophan (W) at the PTC position. The black peaks correspond to non-attributed mass-to-charge ratio (m/z) values. The green peaks are internal fragments that do not contain an extremity of the peptide. b The efficiency of UGA readthrough by DAP decreases with increasing amounts of FTSJ1 but not with increasing amounts of CTU1. Readthrough efficiency was determined by measuring luciferase activity in HeLa cells co-transfected with the firefly luciferase construct described in Fig. 1b and with increasing amounts of an expression vector for either FTSJ1 or CTU1 (0.5, 1, or 2 µg). The empty expression vector (E.v.) was used as control. The cells were then exposed to 0, 0.39, 0.78, 1.56, 6.25, 25, 100, 300, or 600 µM DAP for 24 h before measuring the luciferase activity. The experiment was performed twice and the results of both experiments (Exp1 and Exp2) are shown. c Western blot analysis of FTSJ1 and CTU1. d 2′-O-methylation analysis of tRNATrp, tRNASER, and tRNAGLN by RiboMethSeq. HeLa cells were incubated for 24 h with DMSO or with 25 µM DAP. A MethScore was attributed to each tRNA 2′-O-methylation in tRNA purified from HeLa cells treated with DAP (dark green histogram) or DMSO (light green histogram). The results of three independent experiments are indicated with the symbols square, point, or triangle. e Analysis of DAP affinity column eluates shows that DAP interacts with FTSJ1. HeLa-cell extract was incubated with DAP covalently bound to beads or with beads alone. Proteins bound to the columns were eluted with excess DAP and the eluates analyzed by western blot. A molecular weight marker is indicated to the left of each gel. p-values (p) were calculated using Student’s t-test. Source data are provided as a Source Data file.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Medaka fish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab86057 was affinity purified using an epitope specific to RENT1/hUPF1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RENT1/hUPF1 also known as regulator of nonsense transcripts 1 or human UPF1 is an important factor in RNA surveillance mechanisms. It is an ATP-dependent helicase with a molecular mass of approximately 130 kDa. This protein is ubiquitously expressed in various tissues highlighting its importance across different biological systems. Mechanically UPF1 plays an essential role in the nonsense-mediated mRNA decay (NMD) pathway where it interacts with other core NMD factors to identify and degrade mRNAs containing premature stop codons.

Biological function summary

RENT1/hUPF1 ensures the quality control of mRNA by facilitating the rapid degradation of defective transcripts and thereby preventing the synthesis of potentially harmful truncated proteins. As part of the NMD complex UPF1 partners with proteins like UPF2 and UPF3 to form a surveillance mechanism that maintains cellular homeostasis. The complex cooperates to distinguish transcripts that require elimination from those necessary for normal cellular function.

Pathways

RENT1/hUPF1 integrates into essential cellular pathways beyond just NMD. The protein also links to the mRNA decay and translation initiation pathways playing significant roles alongside proteins such as SMG1 and eRF1. In these pathways UPF1 regulates gene expression by modulating mRNA stability and protein synthesis ensuring that only correctly processed mRNAs translate into proteins.

RENT1/hUPF1 connects to conditions like neurodevelopmental disorders and cancer. Dysregulation of UPF1 activity can lead to improper mRNA decay contributing to the accumulation of faulty transcripts which may drive disease pathogenesis. In particular UPF1 alterations are linked to the disruption of pathways involving key proteins like p53 which are critical in tumor suppression. Understanding UPF1's role in these disorders could present opportunities for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs (PubMed : 11163187, PubMed : 16086026, PubMed : 18172165, PubMed : 21145460, PubMed : 21419344, PubMed : 24726324). Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD (PubMed : 11544179, PubMed : 25220460). Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (PubMed : 19417104). In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD (PubMed : 21419344). Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors (PubMed : 12554878). UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways (PubMed : 18447585). Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2 (PubMed : 16086026, PubMed : 18172165). For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed : 18447585, PubMed : 25220460). The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (PubMed : 21145460). Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA harboring long 3'UTR by inducing the NMD machinery (By similarity). Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA (PubMed : 30218034).

See full target information UPF1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 7: PubMed38986569

2024

Substrate diversity of NSUN enzymes and links of 5-methylcytosine to mRNA translation and turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Guarnacci,Pei-Hong Zhang,Madhu Kanchi,Yu-Ting Hung,Hanrong Lin,Nikolay E Shirokikh,Li Yang,Thomas Preiss

The Journal of biological chemistry 299:104577 PubMed36871759

2023

Serine 970 of RNA helicase MOV10 is phosphorylated and controls unfolding activity and fate of mRNAs targeted for AGO2-mediated silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Aatiqa Nawaz,Phillip J Kenny,Temirlan Shilikbay,Matt Reed,Olga Stuchlik,Jan Pohl,Stephanie Ceman

Nucleic acids research 49:11022-11037 PubMed34634811

2021

A role for AKT1 in nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Martine Palma,Catherine Leroy,Sophie Salomé-Desnoulez,Elisabeth Werkmeister,Rebekah Kong,Marc Mongy,Hervé Le Hir,Fabrice Lejeune

Molecular biology of the cell 32:ar38 PubMed34586879

2021

Selective destabilization of polypeptides synthesized from NMD-targeted transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Chu,Qing Feng,Yang Lim,Sichen Shao

Nature communications 11:1509 PubMed32198346

2020

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Carole Trzaska,Séverine Amand,Christine Bailly,Catherine Leroy,Virginie Marchand,Evelyne Duvernois-Berthet,Jean-Michel Saliou,Hana Benhabiles,Elisabeth Werkmeister,Thierry Chassat,Romain Guilbert,David Hannebique,Anthony Mouray,Marie-Christine Copin,Pierre-Arthur Moreau,Eric Adriaenssens,Andreas Kulozik,Eric Westhof,David Tulasne,Yuri Motorin,Sylvie Rebuffat,Fabrice Lejeune

Journal of cell science 130:3009-3022 PubMed28743738

2017

Premature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jieshuang Jia,Elisabeth Werkmeister,Sara Gonzalez-Hilarion,Catherine Leroy,Dieter C Gruenert,Frank Lafont,David Tulasne,Fabrice Lejeune

Molecular cell 67:239-251.e6 PubMed28669802

2017

The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 Ubiquitin Ligase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Feng,Sujatha Jagannathan,Robert K Bradley

View all publications

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Anti-RENT1/hUPF1 antibody

Immunoprecipitation - Anti-RENT1/hUPF1 antibody (AB86057)

Western blot - Anti-RENT1/hUPF1 antibody (AB86057)

Western blot - Anti-RENT1/hUPF1 antibody (AB86057)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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