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AB254613

Anti-REP-2 antibody

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(1 Publication)

Rabbit Polyclonal REP-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CHML aa 100-200.

View Alternative Names

REP2, CHML, Rab proteins geranylgeranyltransferase component A 2, Choroideremia-like protein, Rab escort protein 2, REP-2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-REP-2 antibody (AB254613)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-REP-2 antibody (AB254613)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling REP-2 using ab254613 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REP-2 antibody (AB254613)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REP-2 antibody (AB254613)

Paraffin-embedded human cerebellum tissue stained for REP-2 using ab254613 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-REP-2 antibody (AB254613)
  • WB

Supplier Data

Western blot - Anti-REP-2 antibody (AB254613)

All lanes:

Western blot - Anti-REP-2 antibody (ab254613) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CHML aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P26374

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REP-2 also known by some researchers as Rab escort protein 2 acts mechanically by facilitating the proper prenylation and trafficking of Rab proteins. These Rab proteins are small GTPases essential for vesicular transport processes. REP-2 has a molecular weight of approximately 75 kDa and is expressed in various tissues with particularly high levels in the brain and pancreas. Its main role involves binding to and transferring newly synthesized Rab proteins to the Rab geranylgeranyltransferase complex for prenylation.
Biological function summary

REP-2 functions in processes involving Rab protein maturation and membrane trafficking. It is a critical component of the Rab escort complex working alongside other members to stabilize the formation of Rab-GTP which subsequently influences intracellular vesicle transport. By ensuring the proper localization and function of Rab GTPases REP-2 supports numerous cellular processes including endocytosis exocytosis and organelle motility which are fundamental to regular cell operation.

Pathways

REP-2 operates within the vesicular trafficking pathways that are vital for cellular homeostasis and signal transduction. It forms part of the Rab protein cycle and interacts closely with the Rab geranylgeranyltransferase helping the prenylation of newly made Rab proteins. This function ties it to the broader network of proteins including Rab1 and Rab3 where REP-2 assists in securing their role in the intricate machinery of vesicular movement and fusion within the cell.

REP-2 has connections to certain neurological and metabolic diseases. It has been linked with choroideremia a retinal degenerative disease where dysfunctional REP-2 leads to impaired Rab function. Such dysfunction results in defective vesicular transport causing the gradual degeneration of photoreceptors. Additionally mutations affecting REP-2 relate to some neurodegenerative disorders highlighting its interaction with Rab27a another protein integral to similar cellular pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
See full target information CHML
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37604583

2023

Tumor necrosis factor mediates USE1-independent FAT10ylation under inflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Schnell,Alina Zubrod,Nicola Catone,Johanna Bialas,Annette Aichem
View all publications
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Product promise

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