Rabbit Polyclonal REP-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHML aa 100-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CHML aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P26374
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Alternative names
REP2, CHML, Rab proteins geranylgeranyltransferase component A 2, Choroideremia-like protein, Rab escort protein 2, REP-2
Recommended products
Rabbit Polyclonal REP-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHML aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CHML aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P26374
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
REP-2 also known by some researchers as Rab escort protein 2 acts mechanically by facilitating the proper prenylation and trafficking of Rab proteins. These Rab proteins are small GTPases essential for vesicular transport processes. REP-2 has a molecular weight of approximately 75 kDa and is expressed in various tissues with particularly high levels in the brain and pancreas. Its main role involves binding to and transferring newly synthesized Rab proteins to the Rab geranylgeranyltransferase complex for prenylation.
- Biological function summary
REP-2 functions in processes involving Rab protein maturation and membrane trafficking. It is a critical component of the Rab escort complex working alongside other members to stabilize the formation of Rab-GTP which subsequently influences intracellular vesicle transport. By ensuring the proper localization and function of Rab GTPases REP-2 supports numerous cellular processes including endocytosis exocytosis and organelle motility which are fundamental to regular cell operation.
- Pathways
REP-2 operates within the vesicular trafficking pathways that are vital for cellular homeostasis and signal transduction. It forms part of the Rab protein cycle and interacts closely with the Rab geranylgeranyltransferase helping the prenylation of newly made Rab proteins. This function ties it to the broader network of proteins including Rab1 and Rab3 where REP-2 assists in securing their role in the intricate machinery of vesicular movement and fusion within the cell.
- Associated diseases and disorders
REP-2 has connections to certain neurological and metabolic diseases. It has been linked with choroideremia a retinal degenerative disease where dysfunctional REP-2 leads to impaired Rab function. Such dysfunction results in defective vesicular transport causing the gradual degeneration of photoreceptors. Additionally mutations affecting REP-2 relate to some neurodegenerative disorders highlighting its interaction with Rab27a another protein integral to similar cellular pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REP-2 antibody (ab254613)
Paraffin-embedded human cerebellum tissue stained for REP-2 using ab254613 at 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-REP-2 antibody (ab254613)
All lanes: Western blot - Anti-REP-2 antibody (ab254613) at 0.4 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (human brain glioma cell line) cell lysate
Lane 3: Human plasma
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Predicted band size: 74 kDa
Immunocytochemistry/ Immunofluorescence - Anti-REP-2 antibody (ab254613)
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling REP-2 using ab254613 at 4µg/ml (green) in ICC/IF.
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