AB137834

Anti-Reptin/TIP49B/RUVB2 antibody

Name: Anti-Reptin/TIP49B/RUVB2 antibody (ab137834) | Abcam
Brand: Abcam Limited
SKU: AB137834
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Reptin/TIP49B/RUVB2 antibody. Suitable for WB, ICC/IF, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RUVBL2 aa 1 to C-terminus.

View Alternative Names

INO80J, TIP48, TIP49B, CGI-46, RUVBL2, RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, INO80 complex subunit J, Repressing pontin 52, TIP49b, TIP60-associated protein 54-beta, 48 kDa TBP-interacting protein, ECP-51, Reptin 52, TAP54-beta

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, labeling RUVBL2 using ab137834 at a 1/200 dilution.
The lower image is merged with a DNA probe.

Supplier Data

Immunoprecipitation - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Immunoprecipitation of RUVBL2 protein from 293T whole cell extracts using 5 µg of ab137834.
Western blot analysis was performed using ab137834.

All lanes:

Immunoprecipitation - Anti-Reptin/TIP49B/RUVB2 antibody (ab137834) at 5 µg

Lane 1:

293T whole cell extracts with control IgG

Lane 2:

Immunoprecipitated 293T whole cell extracts using ab137834

Secondary

All lanes:

Anti Rabbit IgG

Predicted band size: 51 kDa

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Unknown

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

10% SDS PAGE

All lanes:

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab137834) at 1/1000 dilution

All lanes:

H2199 whole cell lysate at 30 µg

Predicted band size: 51 kDa

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CiteAb

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Reptin/TIP49B/RUVB2 western blot using anti-Reptin/TIP49B/RUVB2 antibody ab137834. Publication image and figure legend from Raymond, A. A., Benhamouche, S., et al., 2015, PLoS One, PubMed 25875766.

ab137834 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137834 please see the product overview.

Effect of Reptin depletion on signaling intermediates upstream of H2AX phosphorylation.(A) Protein expression of DNA-PKcs and ATM after Reptin depletion were analyzed by Western Blot on whole cell extracts 4 days after Reptin silencing with doxycycline (sh Rep +) (n = 6 and n = 3 for DNA-PKcs and ATM, respectively : * p<0.05 by Mann and Whitney test) (B) Phosphorylation of CHK2 after Reptin depletion and 2h after gamma ray irradiation (+ IRR) was detected by Western Blot and normalized on total CHK2 (n = 4 : *** p<0.001 by One-way ANOVA followed by Bonferroni test) (C) RNA was extracted 4 days after Reptin silencing and expression of DNA-PKcs and ATM mRNAs was analyzed by RT-qPCR. (n = 3).

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human RUVBL2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y230

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reptin also known as TIP49B or RUVB2 is an ATPase with a mass of approximately 52 kDa. It is expressed ubiquitously across various tissues playing significant roles in cellular processes. Mechanically Reptin functions as part of a complex that facilitates chromatin remodeling and transcription regulation. It often works in conjunction with its paralog Pontin/TIP49A/RUVB1 forming a dodecameric ring structure essential for its activity.

Biological function summary

Reptin plays essential roles in modulating gene expression and contributing to the assembly of ribonucleoprotein complexes. It forms part of the chromatin remodeling complexes such as INO80 and TIP60 participating in DNA repair processes and transcriptional regulation. Its function in these complexes makes it indispensable for maintaining genomic integrity and controlling cell cycle progression.

Pathways

Various critical cellular pathways involve Reptin's biological function. It is an important player in the Wnt/beta-catenin signaling pathway where it regulates the transcription of target genes. Additionally Reptin associates with the Myc pathway contributing to oncogenic transformation. In these pathways Reptin interacts with proteins like beta-catenin and c-Myc integrating signals necessary for cell proliferation and differentiation.

Aberrant Reptin activity links to cancer and neurological disorders. Overexpression of Reptin has strong connections to tumor progression and metastasis in cancers including colorectal and lung cancer. In these diseases Reptin partners with proteins such as c-Myc augmenting its transcriptional activity to promote cell growth. Additionally Reptin's dysregulation may influence neurodegenerative conditions by disrupting protein complexes involved in maintaining neural functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed : 10428817, PubMed : 17157868, PubMed : 33205750). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed : 14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed : 14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400 (PubMed : 14966270). NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed : 14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed : 24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed : 16230350, PubMed : 21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed : 10882073, PubMed : 16014379). May also inhibit the transcriptional activity of ATF2 (PubMed : 11713276). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes (PubMed : 25652260). May play a role in regulating the composition of the U5 snRNP complex (PubMed : 28561026).

See full target information RUVBL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0123333 PubMed25875766

2015

Reptin regulates DNA double strand breaks repair in human hepatocellular carcinoma.

Applications

Species

Human

Anne-Aurélie Raymond,Samira Benhamouche,Véronique Neaud,Julie Di Martino,Joaquim Javary,Jean Rosenbaum

View all publications

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Product promise

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Anti-Reptin/TIP49B/RUVB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Immunoprecipitation - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB137834)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise