Rabbit Polyclonal Reptin/TIP49B/RUVB2 antibody. Suitable for WB, ICC/IF and reacts with Zebrafish, Human samples. Cited in 17 publications.
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Publications
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- Journal of cell science 135:2022Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChris Y Cheung,Ting-Ting Huang,Ning Chow,Shuqi Zhang,Yanxiang Zhao,Mary P Chau,Wing Cheung Chan,Catherine C L Wong,Daniela Boassa,Sebastien Phan,Mark H Ellisman,John R Yates,SongXiao Xu,Zicheng Yu,Yajing Zhang,Rui Zhang,Ling Ling Ng,Ben C B KoPubMed 35635291
- Cell chemical biology 27:105-121.e142019RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul Yenerall,Amit K Das,Shan Wang,Rahul K Kollipara,Long Shan Li,Pamela Villalobos,Josiah Flaming,Yu-Fen Lin,Kenneth Huffman,Brenda C Timmons,Collin Gilbreath,Rajni Sonavane,Lisa N Kinch,Jaime Rodriguez-Canales,Cesar Moran,Carmen Behrens,Makoto Hirasawa,Takehiko Takata,Ryo Murakami,Koichi Iwanaga,Benjamin P C Chen,Nick V Grishin,Ganesh V Raj,Ignacio I Wistuba,John D Minna,Ralf KittlerPubMed 31883965
- Nature communications 8:156152017R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.Applications:WBReactive species:Unspecified reactive speciesPhilippe Cloutier et. al.PubMed 28561026
- Autophagy 13:302-3212016FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatteo Da Ros,Tiina Lehtiniemi,Opeyemi Olotu,Daniel Fischer,Fu-Ping Zhang,Helena Vihinen,Eija Jokitalo,Anu Sironen,Jorma Toppari,Noora KotajaPubMed 27929729
- Molecular oncology 10:1344-622016Proteomic characterization of peroxisome proliferator-activated receptor-γ (PPARγ) overexpressing or silenced colorectal cancer cells unveils a novel protein network associated with an aggressive phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Rita Milone et. al.PubMed 27499265
- Molecular and cellular biology 36:886-992015A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRiyaz A Mir et. al.PubMed 26711270
- FEBS letters 589:1951-72015Reptin physically interacts with p65 and represses NF-κB activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Qiu et. al.PubMed 25957047
- Chemical science 6:3109-31162015Discovery of a novel ligand that modulates the protein-protein interactions of the AAA+ superfamily oncoprotein reptin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlan R Healy et. al.PubMed 28706685
- PloS one 8:e716632013RuvBL2 is involved in histone deacetylase inhibitor PCI-24781-induced cell death in SK-N-DZ neuroblastoma cells.
- FEBS letters 586:435-412012RUVBL2 is a novel repressor of ARF transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChongwei Xie et. al.PubMed 22285491
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed:10428817, PubMed:17157868). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed:14966270). This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed:14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed:14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400 (PubMed:14966270). NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed:14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed:24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed:16230350, PubMed:21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed:10882073, PubMed:16014379). May also inhibit the transcriptional activity of ATF2 (PubMed:11713276). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes (PubMed:25652260). May play a role in regulating the composition of the U5 snRNP complex (PubMed:28561026).
Alternative names
RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, INO80 complex subunit J, Repressing pontin 52, TIP49b, TIP60-associated protein 54-beta, 48 kDa TBP-interacting protein, ECP-51, Reptin 52, TAP54-beta, TIP49B, RUVBL2, INO80J, TIP48, CGI-46
Recommended products
Rabbit Polyclonal Reptin/TIP49B/RUVB2 antibody. Suitable for WB, ICC/IF and reacts with Zebrafish, Human samples. Cited in 17 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Reptin also known as TIP49B or RUVB2 is an ATPase with a mass of approximately 52 kDa. It is expressed ubiquitously across various tissues playing significant roles in cellular processes. Mechanically Reptin functions as part of a complex that facilitates chromatin remodeling and transcription regulation. It often works in conjunction with its paralog Pontin/TIP49A/RUVB1 forming a dodecameric ring structure essential for its activity.
- Biological function summary
Reptin plays essential roles in modulating gene expression and contributing to the assembly of ribonucleoprotein complexes. It forms part of the chromatin remodeling complexes such as INO80 and TIP60 participating in DNA repair processes and transcriptional regulation. Its function in these complexes makes it indispensable for maintaining genomic integrity and controlling cell cycle progression.
- Pathways
Various critical cellular pathways involve Reptin's biological function. It is an important player in the Wnt/beta-catenin signaling pathway where it regulates the transcription of target genes. Additionally Reptin associates with the Myc pathway contributing to oncogenic transformation. In these pathways Reptin interacts with proteins like beta-catenin and c-Myc integrating signals necessary for cell proliferation and differentiation.
- Associated diseases and disorders
Aberrant Reptin activity links to cancer and neurological disorders. Overexpression of Reptin has strong connections to tumor progression and metastasis in cancers including colorectal and lung cancer. In these diseases Reptin partners with proteins such as c-Myc augmenting its transcriptional activity to promote cell growth. Additionally Reptin's dysregulation may influence neurodegenerative conditions by disrupting protein complexes involved in maintaining neural functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)
All lanes: Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 8: U2OS Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)
ICC/IF image of ab36569 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab36569, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)
Detection method was Odyssey, with 10 second exposure.
All lanes: Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/mL
Lane 1: Hela cells treated with reptin-specific siRNA#1, whole cell lysate at 20 µg
Lane 2: Hela cells treated with reptin-specific siRNA#2, whole cell lysate at 20 µg
Lane 3: Hela cells treated with non-specific siRNA, whole cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor® 680 conjugated goat anti-rabbit at 1/7000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55 kDa
Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)
All lanes: Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/mL
Lane 1: Marker
Lane 2: Zebrafish brain homogenate at 20 µg
Lane 3: Zebrafish heart homogenate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 5min
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