Anti-Reptin/TIP49B/RUVB2 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

ICC/IF image of ab36569 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab36569, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• WB

AbReview9198****

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

Detection method was Odyssey, with 10 second exposure.

All lanes:

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/mL

Lane 1:

Hela cells treated with reptin-specific siRNA#1, whole cell lysate at 20 µg

Lane 2:

Hela cells treated with reptin-specific siRNA#2, whole cell lysate at 20 µg

Lane 3:

Hela cells treated with non-specific siRNA, whole cell lysate at 20 µg

Secondary

All lanes:

Alexa Fluor® 680 conjugated goat anti-rabbit at 1/7000 dilution

Predicted band size: 51 kDa

Observed band size: 55 kDa

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This image is courtesy of an anonymous Abreview

• WB

Project

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

All lanes:

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 10 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 10 µg

Lane 4:

Western blot - HEK-293 whole cell lysate (<a href='/en-us/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 10 µg

Lane 5:

Hep G2 whole cell lysate (<a href='/en-us/products/unavailable/hep-g2-whole-cell-lysate-ab7900'>ab7900</a>) at 10 µg

Lane 6:

MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

U2OS Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 51 kDa

Observed band size: 52 kDa

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• WB

Unknown

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

All lanes:

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Zebrafish brain homogenate at 20 µg

Lane 3:

Zebrafish heart homogenate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

true

Exposure time: 5min

• WB

CiteAb

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

Reptin/TIP49B/RUVB2 western blot using anti-Reptin/TIP49B/RUVB2 antibody ab36569. Publication image and figure legend from Cloutier, P., Poitras, C., et al., 2017, Nat Commun, PubMed 28561026.

ab36569 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab36569 please see the product overview.

ZNHIT2 and RUVBL2 regulate the composition of the U5 snRNP.(a) Workflow of triple SILAC experiments. (b) Expression assessment of various U5 snRNP subunits following treatment with siZNHIT2 or siRUVBL2. (c–f) Interpretation of the SILAC data on scatterplots. Intensity ratio of medium-labelled proteins over unlabelled proteins (M/L) on the X axis denotes IP enrichment. Intensity ratio of medium-labelled proteins over heavy-labelled proteins (M/H) on the Y axis represents experimental variations. Marked proteins in the upper right quadrant (X>1, Y>1) are proteins enriched in the IP that were less abundant following siRNA treatment. Position of the protein used for IP is shown in all experiments.

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