Rabbit Recombinant Monoclonal Resistin antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells (By similarity). Potentially links obesity to diabetes (By similarity). Promotes chemotaxis in myeloid cells (PubMed:15064728).
FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, HXCP1, UNQ407/PRO1199, RSTN, FIZZ3, RETN, Resistin, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, ADSF
Rabbit Recombinant Monoclonal Resistin antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR23334-171
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab275887 is the carrier-free version of Anti-Resistin antibody [EPR23334-171] ab275878.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.
Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.
Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.
Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Resistin antibody [EPR23334-171] ab275878, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:16532325, 14550293).
Low expression: THP-1 (PMID:16532325 ).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Resistin antibody [EPR23334-171] (Anti-Resistin antibody [EPR23334-171] ab275878) at 1/1000 dilution
Lane 1: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Lane 2: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
This data was developed using Anti-Resistin antibody [EPR23334-171] ab275878, the same antibody clone in a different buffer formulation.
Resistin was immunoprecipitated from 0.35 mg U937 (human histiocytic lymphoma monocyte) whole cell lysate with Anti-Resistin antibody [EPR23334-171] ab275878 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Resistin antibody [EPR23334-171] ab275878 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: U937 whole cell lysate 10 μg
Lane 2: Anti-Resistin antibody [EPR23334-171] ab275878 IP in U937 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Resistin antibody [EPR23334-171] ab275878 in U937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds.
All lanes: Immunoprecipitation - Anti-Resistin antibody [EPR23334-171] (Anti-Resistin antibody [EPR23334-171] ab275878)
Predicted band size: 11 kDa
Observed band size: 11 kDa
This data was developed using Anti-Resistin antibody [EPR23334-171] ab275878, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Resistin antibody [EPR23334-171] (Anti-Resistin antibody [EPR23334-171] ab275878) at 1/1000 dilution
All lanes: Human white fat tissue lysate at 20 µg
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
This data was developed using Anti-Resistin antibody [EPR23334-171] ab275878, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Resistin antibody [EPR23334-171] (Anti-Resistin antibody [EPR23334-171] ab275878) at 1/1000 dilution
All lanes: Recombinant human Resistin protein, 20 ng
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 11 kDa
This data was developed using Anti-Resistin antibody [EPR23334-171] ab275878, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U937 (human histiocytic lymphoma monocyte) cells labelling Resistin with Anti-Resistin antibody [EPR23334-171] ab275878 at 1/500 dilution (0.1μg)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
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