Anti-Respiratory Syncytial Virus M2-1 Protein antibody [EPR28649-58]

4 product images

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  Indirect ELISA - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [EPR28649-58] (ab323319)

  Indirect ELISA analysis of ab323319 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.

  Antigen: Respiratory Syncytial Virus M2-1 protein, Respiratory Syncytial Virus M2-1 peptide.

  Antigen concentration: 1000 ng/ml

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  Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [EPR28649-58] (ab323319)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Respiratory Syncytial Virus M2-1 Protein  with ab323319 at 1/4000 (0.129 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a Human respiratory syncytial virus A (HRSVA) and Human respiratory syncytial virus B (HRSVB) M2-1 protein expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [EPR28649-58] (ab323319)

  Immunohistochemical analysis of paraffin-embedded (A) 293T cells transfected with a Human respiratory syncytial virus A M2-1 protein expression vector containing a his-tag cell pellet. (B) 293T cells transfected with a Human respiratory syncytial virus B M2-1 protein expression vector containing a his-tag cell pellet. (C) 293T transfected with an empty vector containing a his-tag cell pellet. tissue labeling Respiratory Syncytial Virus M2-1 Protein  with ab323319 at 1/10000 (0.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in (A) 293T transfected with a HRSVA M2-1 expression vector containing a his-tag cell pellet and (B) 293T transfected with a HRSVB M2-1 expression vector containing a his-tag cell pellet. No staining in (C) 293T transfected with an empty vector containing a his-tag cell pellet.
  
  The section was incubated with ab323319 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Western blot - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [EPR28649-58] (ab323319)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [EPR28649-58] (ab323319) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a Human respiratory syncytial virus A M2-1 protein expression vector containing a his-tag, whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a Human respiratory syncytial virus B M2-1 protein expression vector containing a his-tag, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 22 kDa, 36 kDa

  Exposure time: 3s