Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5]

• WB

PubMed

Western blot - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] (AB94805)

RSV mutants show expected patterns of G, F, and M2-1 expression.

Western blotting of Vero cell lysates infected with RSV ΔG, ΔG C-term, and wild type (MSA1).

(Figure C shown) Detection of M2-1 using anti-M2-1 antibody ab94805.

Cell lysates from virus cultures grown for 6 days on Vero cells were subjected to SDS-PAGE, transferred to membranes and probed with anti-M2-1 antibody (Abcam, ab94805), and incubated with a secondary goat anti-mouse alkaline phosphatase conjugate. Anti-G western blot was done with rabbit anti-RSV-G polyclonal and incubated with goat anti-rabbit secondary antibody (Abcam, ab97048). Bands were visualized using BCIP/NBT. The molecular weight marker used was Precision Plus Protein Dual Color Standards.

All lanes:

Western blot - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] (ab94805)

Predicted band size: 22 kDa

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Jeong et al PLoS One. 2015 Jun 24;10(6):e0130517. doi: 10.1371/journal.pone.0130517. eCollection 2015. S1 File. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/